Telomeres thrown for a loop.

abstract：:MeCP2 is an essential transcriptional repressor that mediates gene silencing through binding to methylated DNA. Binding specificity has been thought to depend on hydrophobic interactions between cytosine methyl groups and a hydrophobic patch within the methyl-CpG-binding domain (MBD). X-ray analysis of a methylated DN...

journal_title：Molecular cell

authors： Ho KL,McNae IW,Schmiedeberg L,Klose RJ,Bird AP,Walkinshaw MD

更新日期：2008-02-29 00:00:00

abstract：:How proteasomal ATPases stimulate the gate opening of 20S proteasomes is a longstanding question. In the September 7 issue of Molecular Cell, Smith et al. (2007) describe the conserved "HbYX" motif as the key to proteasome gate opening. ...

journal_title：Molecular cell

authors： Saeki Y,Tanaka K

更新日期：2007-09-21 00:00:00

abstract：:In response to hyperosmotic environments, most eukaryotic cells activate a specialized mitogen-activated protein (MAP) kinase pathway. In S. cerevisiae, the key protein kinase, Hog1, coordinates the transcriptional induction of a variety of genes devoted to osmoadaptation and general stress protection. Depending on th...

journal_title：Molecular cell

authors： Alepuz PM,Jovanovic A,Reiser V,Ammerer G

更新日期：2001-04-01 00:00:00

abstract：:Signaling by TGFbeta regulates the expression of hundreds of genes. The rapid repression of c-Myc stands out because of its roles in growth control and cancer. Recent work shows that c-Myc repression by TGFbeta is mediated by the nuclear translocation of a novel, preformed complex composed of Smad3, E2F4 or E2F5, and ...

journal_title：Molecular cell

authors： Kowalik TF

更新日期：2002-07-01 00:00:00

abstract：:In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq...

journal_title：Molecular cell

authors： Tree JJ,Granneman S,McAteer SP,Tollervey D,Gally DL

更新日期：2014-07-17 00:00:00

abstract：:Brr2 is a unique DExD/H box protein required for catalytic activation and disassembly of the spliceosome. It contains two tandem helicase cassettes that both comprise dual RecA-like domains and a noncanonical Sec63 unit. The latter may bestow the enzyme with unique properties. We have determined crystal structures of ...

journal_title：Molecular cell

authors： Pena V,Jovin SM,Fabrizio P,Orlowski J,Bujnicki JM,Lührmann R,Wahl MC

更新日期：2009-08-28 00:00:00

abstract：:Hydroxyl radical footprinting and directed probing from Fe(II)-derivatized IF3 have been used to map the interaction of IF3 relative to 16S rRNA and tRNA(Met)(f) in the 30S ribosomal subunit. Our results place the two domains of IF3 on opposite sides of the initiator tRNA, with the C domain at the platform interface a...

journal_title：Molecular cell

authors： Dallas A,Noller HF

更新日期：2001-10-01 00:00:00

abstract：:We generated knockout mice for MCM8 and MCM9 and show that deficiency for these genes impairs homologous recombination (HR)-mediated DNA repair during gametogenesis and somatic cells cycles. MCM8(-/-) mice are sterile because spermatocytes are blocked in meiotic prophase I, and females have only arrested primary folli...

journal_title：Molecular cell

authors： Lutzmann M,Grey C,Traver S,Ganier O,Maya-Mendoza A,Ranisavljevic N,Bernex F,Nishiyama A,Montel N,Gavois E,Forichon L,de Massy B,Méchali M

更新日期：2012-08-24 00:00:00

abstract：:Polycomb group (PcG) proteins exert essential functions in the most disparate biological processes. The contribution of PcG proteins to cell commitment and differentiation relates to their ability to repress transcription of developmental regulators in embryonic stem (ES) cells and in committed cell lineages, includin...

journal_title：Molecular cell

authors： Juan AH,Kumar RM,Marx JG,Young RA,Sartorelli V

更新日期：2009-10-09 00:00:00

abstract：:Deletion of elongation factor-like 1 (Efl1p), a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2, results in nucle(ol)ar pre-rRNA processing and pre-60S subunits export defects. Efl1p interacts genetically with Tif6p, a nucle(ol)ar protein stably associated with pre-60S subunits and required for t...

journal_title：Molecular cell

authors： Senger B,Lafontaine DL,Graindorge JS,Gadal O,Camasses A,Sanni A,Garnier JM,Breitenbach M,Hurt E,Fasiolo F

更新日期：2001-12-01 00:00:00

abstract：:DNA replication introduces a dosage imbalance between early and late replicating genes. In budding yeast, buffering gene expression against this imbalance depends on marking replicated DNA by H3K56 acetylation (H3K56ac). Whether additional processes are required for suppressing transcription from H3K56ac-labeled DNA r...

journal_title：Molecular cell

authors： Voichek Y,Mittelman K,Gordon Y,Bar-Ziv R,Lifshitz Smit D,Shenhav R,Barkai N

更新日期：2018-06-21 00:00:00

abstract：:Crossover recombination is essential for accurate chromosome segregation during meiosis. The MutSγ complex, Msh4-Msh5, facilitates crossing over by binding and stabilizing nascent recombination intermediates. We show that these activities are governed by regulated proteolysis. MutSγ is initially inactive for crossing ...

journal_title：Molecular cell

authors： He W,Rao HBDP,Tang S,Bhagwat N,Kulkarni DS,Ma Y,Chang MAW,Hall C,Bragg JW,Manasca HS,Baker C,Verhees GF,Ranjha L,Chen X,Hollingsworth NM,Cejka P,Hunter N

更新日期：2020-04-02 00:00:00

abstract：:The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S...

journal_title：Molecular cell

authors： Dionne I,Nookala RK,Jackson SP,Doherty AJ,Bell SD

更新日期：2003-01-01 00:00:00

abstract：:ADP-ribosylation is a post-translational modification where single units (mono-ADP-ribosylation) or polymeric chains (poly-ADP-ribosylation) of ADP-ribose are conjugated to proteins by ADP-ribosyltransferases. This post-translational modification and the ADP-ribosyltransferases (also known as PARPs) responsible for it...

journal_title：Molecular cell

authors： Daniels CM,Ong SE,Leung AK

更新日期：2015-06-18 00:00:00

abstract：:In this issue of Molecular Cell, Roberts-Galbraith and colleagues report that a key cytokinetic regulator in fission yeast, Cdc15, is phosphorylated on numerous sites that collectively, but not individually, control its oligomerization state and its associations with the plasma membrane and interacting proteins. ...

journal_title：Molecular cell

authors： Glotzer M

更新日期：2010-07-09 00:00:00

abstract：:There are few tools available for dissecting and elucidating the functions of DNA satellites and other nongenic DNA. To address this, we have explored the experimental potential of DNA sequence-specific drugs containing pyrrole and imidazole amino acids (polyamides). Compounds were synthesized that target different Dr...

journal_title：Molecular cell

authors： Janssen S,Durussel T,Laemmli UK

更新日期：2000-11-01 00:00:00

abstract：:Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor e...

journal_title：Molecular cell

authors： Wells SE,Hillner PE,Vale RD,Sachs AB

更新日期：1998-07-01 00:00:00

abstract：:Eukaryotic protein kinases are generally classified as being either tyrosine or serine-threonine specific. Though not evident from inspection of their primary sequences, many serine-threonine kinases display a significant preference for serine or threonine as the phosphoacceptor residue. Here we show that a residue lo...

journal_title：Molecular cell

authors： Chen C,Ha BH,Thévenin AF,Lou HJ,Zhang R,Yip KY,Peterson JR,Gerstein M,Kim PM,Filippakopoulos P,Knapp S,Boggon TJ,Turk BE

更新日期：2014-01-09 00:00:00

abstract：:Centromeres are specialized chromatin domains specified by the centromere-specific CENP-A nucleosome. The stable inheritance of vertebrate centromeres is an epigenetic process requiring deposition of new CENP-A nucleosomes by HJURP. We show HJURP is recruited to centromeres through a direct interaction between the HJU...

journal_title：Molecular cell

authors： Nardi IK,Zasadzińska E,Stellfox ME,Knippler CM,Foltz DR

更新日期：2016-03-03 00:00:00

abstract：:Signal transducer and activator of transcription 1 (STAT1) is activated by tyrosine phosphorylation upon interferon-γ (IFNγ) stimulation, which results in the expression of genes with antiproliferative and immunomodulatory functions. The inactivation of STAT1 occurs through tyrosine dephosphorylation by the tyrosine p...

journal_title：Molecular cell

authors： Pelzel C,Begitt A,Wenta N,Vinkemeier U

更新日期：2013-04-11 00:00:00

abstract：:Copy-number changes generate phenotypic variability in health and disease. Whether organisms protect against copy-number changes is largely unknown. Here, we show that Saccharomyces cerevisiae monitors the copy number of its ribosomal DNA (rDNA) and rapidly responds to copy-number loss with the clonal amplification of...

journal_title：Molecular cell

authors： Mansisidor A,Molinar T Jr,Srivastava P,Dartis DD,Pino Delgado A,Blitzblau HG,Klein H,Hochwagen A

更新日期：2018-11-01 00:00:00

abstract：:Uncoupling protein 1 (UCP1) mediates nonshivering thermogenesis and, upon cold exposure, is induced in brown adipose tissue (BAT) and subcutaneous white adipose tissue (iWAT). Here, by high-throughput screening using the UCP1 promoter, we identify Zfp516 as a transcriptional activator of UCP1 as well as PGC1α, thereby...

journal_title：Molecular cell

authors： Dempersmier J,Sambeat A,Gulyaeva O,Paul SM,Hudak CS,Raposo HF,Kwan HY,Kang C,Wong RH,Sul HS

更新日期：2015-01-22 00:00:00

abstract：:One of the oldest questions in RNA science is the role of nucleotide modification. Here, the importance of pseudouridine formation (Psi) in the peptidyl transferase center of rRNA was examined by depleting yeast cells of 1-5 snoRNAs that guide a total of six Psi modifications. Translation was impaired substantially wi...

journal_title：Molecular cell

authors： King TH,Liu B,McCully RR,Fournier MJ

更新日期：2003-02-01 00:00:00

abstract：:The convergence of two DNA replication forks creates unique problems during DNA replication termination. In E. coli and SV40, the release of torsional strain by type II topoisomerases is critical for converging replisomes to complete DNA synthesis, but the pathways that mediate fork convergence in eukaryotes are unkno...

journal_title：Molecular cell

authors： Deegan TD,Baxter J,Ortiz Bazán MÁ,Yeeles JTP,Labib KPM

更新日期：2019-04-18 00:00:00

abstract：:tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...

journal_title：Molecular cell

authors： Kothe U,Rodnina MV

更新日期：2007-01-12 00:00:00

abstract：:Alternative inclusion of exons increases the functional diversity of proteins. Among alternatively spliced exons, tissue-specific exons play a critical role in maintaining tissue identity. This raises the question of how tissue-specific protein-coding exons influence protein function. Here we investigate the structura...

journal_title：Molecular cell

authors： Buljan M,Chalancon G,Eustermann S,Wagner GP,Fuxreiter M,Bateman A,Babu MM

更新日期：2012-06-29 00:00:00

abstract：:Nuclear export of mRNA in eukaryotic cells is mediated by soluble transport factors and components of the nuclear pore complex (NPC). The cytoplasmically oriented nuclear pore protein Nup159 plays a critical role in mRNA export through its conserved N-terminal domain (NTD). Here, we report the crystal structure of the...

journal_title：Molecular cell

authors： Weirich CS,Erzberger JP,Berger JM,Weis K

更新日期：2004-12-03 00:00:00

abstract：:Ataxia Telangiectasia Mutated (ATM) signaling is essential for the repair of a subset of DNA double-strand breaks (DSBs); however, its precise role is unclear. Here, we show that < or =25% of DSBs require ATM signaling for repair, and this percentage correlates with increased chromatin but not damage complexity. Impor...

journal_title：Molecular cell

authors： Goodarzi AA,Noon AT,Deckbar D,Ziv Y,Shiloh Y,Löbrich M,Jeggo PA

更新日期：2008-07-25 00:00:00

abstract：:Curli are extracellular functional amyloids that are assembled by enteric bacteria during biofilm formation and host colonization. An efficient secretion system and chaperone network ensures that the major curli fiber subunit, CsgA, does not form intracellular amyloid aggregates. We discovered that the periplasmic pro...

journal_title：Molecular cell

authors： Evans ML,Chorell E,Taylor JD,Åden J,Götheson A,Li F,Koch M,Sefer L,Matthews SJ,Wittung-Stafshede P,Almqvist F,Chapman MR

更新日期：2015-02-05 00:00:00

abstract：:While gapmers efficiently knock down as well as terminate transcription of nascent lncRNAs and mRNAs, Lee and Mendell (2020) and Lai et al. (2020) also demonstrate that Pol II termination is not observed with gapmers targeting the 3' terminal portions of the transcript. ...

journal_title：Molecular cell

authors： Maranon DG,Wilusz J

更新日期：2020-03-05 00:00:00