The pathway for DNA recognition and RNA integration by a group II intron retrotransposon.

Abstract:

:Group II intron RNPs are mobile genetic elements that attack and invade duplex DNA. In this work, we monitor the invasion reaction in vitro and establish a quantitative kinetic framework for the steps of this complex cascade. We find that target site specificity is achieved after DNA binding, which occurs nonspecifically. RNP searches the bound DNA before undergoing a conformational change that is associated with identification of its specific binding site. The study reveals a facile equilibrium between intron invasion and splicing, indicating that RNP invasion of top strand DNA is a relatively unfavorable event. Group II mobility must therefore depend on the trapping of invasion products, potentially through interaction of the intron-encoded protein with the DNA target and/or initiation of reverse transcription.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Aizawa Y,Xiang Q,Lambowitz AM,Pyle AM

doi

10.1016/s1097-2765(03)00069-8

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

795-805

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(03)00069-8

journal_volume

11

pub_type

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