Abstract:
:Group II intron RNPs are mobile genetic elements that attack and invade duplex DNA. In this work, we monitor the invasion reaction in vitro and establish a quantitative kinetic framework for the steps of this complex cascade. We find that target site specificity is achieved after DNA binding, which occurs nonspecifically. RNP searches the bound DNA before undergoing a conformational change that is associated with identification of its specific binding site. The study reveals a facile equilibrium between intron invasion and splicing, indicating that RNP invasion of top strand DNA is a relatively unfavorable event. Group II mobility must therefore depend on the trapping of invasion products, potentially through interaction of the intron-encoded protein with the DNA target and/or initiation of reverse transcription.
journal_name
Mol Celljournal_title
Molecular cellauthors
Aizawa Y,Xiang Q,Lambowitz AM,Pyle AMdoi
10.1016/s1097-2765(03)00069-8subject
Has Abstractpub_date
2003-03-01 00:00:00pages
795-805issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(03)00069-8journal_volume
11pub_type
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