Abstract:
:B and T cell receptor gene assembly by V(D)J recombination is tightly regulated during lymphoid development. The mechanisms involved in this regulation are poorly understood. Here we show that nucleosomal DNA is refractory to V(D)J cleavage. However, the presence of HMG1, a chromatin-associated nonhistone DNA-binding protein, stimulates V(D)J cleavage of nucleosomal templates. This HMG1 stimulation is differentially affected by the rotational or translational positioning of the recombination signal sequence on the histone octamer, with cleavage of the 12 bp spacer RSS showing sensitivity to rotational position and the 23 bp spacer RSS affected by its displacement from the dyad. These results suggest that V(D)J recombination can be modulated by controlling substrate accessibility and cleavage at the level of an individual nucleosome.
journal_name
Mol Celljournal_title
Molecular cellauthors
Kwon J,Imbalzano AN,Matthews A,Oettinger MAdoi
10.1016/s1097-2765(00)80297-xsubject
Has Abstractpub_date
1998-12-01 00:00:00pages
829-39issue
6eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80297-Xjournal_volume
2pub_type
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