Abstract：
:The convergence of two DNA replication forks creates unique problems during DNA replication termination. In E. coli and SV40, the release of torsional strain by type II topoisomerases is critical for converging replisomes to complete DNA synthesis, but the pathways that mediate fork convergence in eukaryotes are unknown. We studied the convergence of reconstituted yeast replication forks that include all core replisome components and both type I and type II topoisomerases. We found that most converging forks stall at a very late stage, indicating a role for additional factors. We showed that the Pif1 and Rrm3 DNA helicases promote efficient fork convergence and completion of DNA synthesis, even in the absence of type II topoisomerase. Furthermore, Rrm3 and Pif1 are also important for termination of plasmid DNA replication in vivo. These findings identify a eukaryotic pathway for DNA replication termination that is distinct from previously characterized prokaryotic mechanisms.
journal_name
Mol Celljournal_title
Molecular cellauthors
Deegan TD,Baxter J,Ortiz Bazán MÁ,Yeeles JTP,Labib KPMdoi
10.1016/j.molcel.2019.01.040subject
Has Abstractpub_date
2019-04-18 00:00:00pages
231-244.e9issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(19)30060-7journal_volume
74pub_type
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