Abstract：
:It has been suspected that cell-cycle progression might be functionally coupled with RNA processing. However, little is known about the role of the precise splicing control in cell-cycle progression. Here, we report that SON, a large Ser/Arg (SR)-related protein, is a splicing cofactor contributing to efficient splicing of cell-cycle regulators. Downregulation of SON leads to severe impairment of spindle pole separation, microtubule dynamics, and genome integrity. These molecular defects result from inadequate RNA splicing of a specific set of cell-cycle-related genes that possess weak splice sites. Furthermore, we show that SON facilitates the interaction of SR proteins with RNA polymerase II and other key spliceosome components, suggesting its function in efficient cotranscriptional RNA processing. These results reveal a mechanism for controlling cell-cycle progression through SON-dependent constitutive splicing at suboptimal splice sites, with strong implications for its role in cancer and other human diseases.
journal_name
Mol Celljournal_title
Molecular cellauthors
Ahn EY,DeKelver RC,Lo MC,Nguyen TA,Matsuura S,Boyapati A,Pandit S,Fu XD,Zhang DEdoi
10.1016/j.molcel.2011.03.014subject
Has Abstractpub_date
2011-04-22 00:00:00pages
185-98issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(11)00214-0journal_volume
42pub_type
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