ERp29 triggers a conformational change in polyomavirus to stimulate membrane binding.

abstract：:Using genome-wide approaches, we have elucidated the regulatory circuitry governed by the XBP1 transcription factor, a key effector of the mammalian unfolded protein response (UPR), in skeletal muscle and secretory cells. We identified a core group of genes involved in constitutive maintenance of ER function in all ce...

journal_title：Molecular cell

authors： Acosta-Alvear D,Zhou Y,Blais A,Tsikitis M,Lents NH,Arias C,Lennon CJ,Kluger Y,Dynlacht BD

更新日期：2007-07-06 00:00:00

abstract：:Clathrin-dependent endocytosis has long been presented as the only efficient mechanism by which transmembrane receptors are internalized. We selectively blocked this process using dominant-negative mutants of Eps15 and showed that clathrin-mediated endocytosis of transferrin was inhibited, while endocytosis of interle...

journal_title：Molecular cell

authors： Lamaze C,Dujeancourt A,Baba T,Lo CG,Benmerah A,Dautry-Varsat A

更新日期：2001-03-01 00:00:00

abstract：:Molecular motors are the long-haul carriers of eukaryotic cells, moving cargos bidirectionally along microtubule tracks. In the December 12th issue of Cell, report that HDAC6, a tubulin deacetylase, functions as an adaptor that links cargos of aggregated protein to the minus end-directed motor, cytoplasmic dynein. ...

journal_title：Molecular cell

authors： Kopito RR

更新日期：2003-12-01 00:00:00

abstract：:The ER-associated degradation (ERAD) pathway serves as an important cellular safeguard by directing incorrectly folded and unassembled proteins from the ER to the proteasome. Still, however, little is known about the components mediating ERAD of membrane proteins. Here we show that the evolutionary conserved rhomboid ...

journal_title：Molecular cell

authors： Fleig L,Bergbold N,Sahasrabudhe P,Geiger B,Kaltak L,Lemberg MK

更新日期：2012-08-24 00:00:00

abstract：:Expression of BRCA1 is commonly decreased in sporadic breast tumors, and this correlates with poor prognosis of breast cancer patients. Here we show that BRCA1 transcripts are selectively enriched in the Argonaute/miR-182 complex and miR-182 downregulates BRCA1 expression. Antagonizing miR-182 enhances BRCA1 protein l...

journal_title：Molecular cell

authors： Moskwa P,Buffa FM,Pan Y,Panchakshari R,Gottipati P,Muschel RJ,Beech J,Kulshrestha R,Abdelmohsen K,Weinstock DM,Gorospe M,Harris AL,Helleday T,Chowdhury D

更新日期：2011-01-21 00:00:00

abstract：:The heterodimeric nuclear cap binding complex (CBC) binds to 5'-capped polymerase II transcripts. It enhances the efficiency of several mRNA maturation steps and is essential for U snRNA nuclear export in multicellular eukaryotes. The 2A crystal structure of human CBC shows that the large subunit, CBP80, comprises thr...

journal_title：Molecular cell

authors： Mazza C,Ohno M,Segref A,Mattaj IW,Cusack S

更新日期：2001-08-01 00:00:00

abstract：:CCR4-NOT is a major effector complex in miRNA-mediated gene silencing. It is recruited to miRNA targets through interactions with tryptophan (W)-containing motifs in TNRC6/GW182 proteins and is required for both translational repression and degradation of miRNA targets. Here, we elucidate the structural basis for the ...

journal_title：Molecular cell

authors： Chen Y,Boland A,Kuzuoğlu-Öztürk D,Bawankar P,Loh B,Chang CT,Weichenrieder O,Izaurralde E

更新日期：2014-06-05 00:00:00

abstract：:Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals a...

journal_title：Molecular cell

authors： Flury V,Georgescu PR,Iesmantavicius V,Shimada Y,Kuzdere T,Braun S,Bühler M

更新日期：2017-07-20 00:00:00

abstract：:E1 enzymes activate ubiquitin or related proteins and pass them to E2 enzymes. A recent structure and associated biochemical studies (Huang et al., 2005) show how an E1 binds its cognate E2 and indicates that large, conformational changes will be an integral component of the E1 reaction cycle. ...

journal_title：Molecular cell

authors： VanDemark AP,Hill CP

更新日期：2005-02-18 00:00:00

abstract：:The structure of an RNA polymerase II/general transcription factor TFIIF complex was determined by cryo-electron microscopy and single particle analysis. Density due to TFIIF was not concentrated in one area but rather was widely distributed across the surface of the polymerase. The largest subunit of TFIIF interacted...

journal_title：Molecular cell

authors： Chung WH,Craighead JL,Chang WH,Ezeokonkwo C,Bareket-Samish A,Kornberg RD,Asturias FJ

更新日期：2003-10-01 00:00:00

abstract：:The Mre11/Rad50/Nbs1 complex initiates double-strand break repair by homologous recombination (HR). Loss of Mre11 or its nuclease activity in mouse cells is known to cause genome aberrations and cellular senescence, although the molecular basis for this phenotype is not clear. To identify the origin of these defects, ...

journal_title：Molecular cell

authors： Hoa NN,Shimizu T,Zhou ZW,Wang ZQ,Deshpande RA,Paull TT,Akter S,Tsuda M,Furuta R,Tsutsui K,Takeda S,Sasanuma H

更新日期：2016-11-03 00:00:00

abstract：:Mutation and prevalence of pathogenic viruses prompt the development of broad-spectrum antiviral strategies. Viperin is a potent antiviral protein that inhibits a broad range of viruses. Unexpectedly, we found that Viperin protein production in epithelium is defective in response to both viruses and interferons (IFNs)...

journal_title：Molecular cell

authors： Yuan Y,Miao Y,Qian L,Zhang Y,Liu C,Liu J,Zuo Y,Feng Q,Guo T,Zhang L,Chen X,Jin L,Huang F,Zhang H,Zhang W,Li W,Xu G,Zheng H

更新日期：2020-02-20 00:00:00

abstract：:We show that the IRF-2 oncoprotein represses virus-induced IFN-beta gene transcription via a novel mechanism. Virus infection induces recruitment of IRF-2 to some of the endogenous IFN-beta enhancers as part of the enhanceosome. Enhanceosomes bearing IRF-2 cannot activate transcription, due to the presence of a domain...

journal_title：Molecular cell

authors： Senger K,Merika M,Agalioti T,Yie J,Escalante CR,Chen G,Aggarwal AK,Thanos D

更新日期：2000-10-01 00:00:00

abstract：:Circadian rhythms in mammals are generated by a transcriptional negative feedback loop that is driven primarily by oscillations of PER and CRY, which inhibit their own transcriptional activators, CLOCK and BMAL1. Current models posit that CRY is the dominant repressor, while PER may play an accessory role. In this stu...

journal_title：Molecular cell

authors： Chen R,Schirmer A,Lee Y,Lee H,Kumar V,Yoo SH,Takahashi JS,Lee C

更新日期：2009-11-13 00:00:00

abstract：:Long DNA palindromes are sites of genome instability (deletions, amplification, and translocations) in both prokaryotic and eukaryotic cells. In Escherichia coli, genetic evidence has suggested that they are sites of DNA cleavage by the SbcCD complex that can be repaired by homologous recombination. Here we obtain in ...

journal_title：Molecular cell

authors： Eykelenboom JK,Blackwood JK,Okely E,Leach DR

更新日期：2008-03-14 00:00:00

abstract：:The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S...

journal_title：Molecular cell

authors： Dionne I,Nookala RK,Jackson SP,Doherty AJ,Bell SD

更新日期：2003-01-01 00:00:00

abstract：:When inappropriate DNA structures arise, they are sensed by DNA structure-dependent checkpoint pathways and subsequently repaired. Recruitment of checkpoint proteins to such structures precedes recruitment of proteins involved in DNA metabolism. Thus, checkpoints can regulate DNA metabolism. We show that fission yeast...

journal_title：Molecular cell

authors： Furuya K,Miyabe I,Tsutsui Y,Paderi F,Kakusho N,Masai H,Niki H,Carr AM

更新日期：2010-11-24 00:00:00

abstract：:The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and GSK3beta, forms a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of beta-catenin by the proteasome. Siah-1, the human homolog of Drosophila seven in absentia, is a p53-inducible mediator of cell c...

journal_title：Molecular cell

authors： Liu J,Stevens J,Rote CA,Yost HJ,Hu Y,Neufeld KL,White RL,Matsunami N

更新日期：2001-05-01 00:00:00

abstract：:Stress granules are mRNA-protein assemblies formed from nontranslating mRNAs. Stress granules are important in the stress response and may contribute to some degenerative diseases. Here, we describe the stress granule transcriptome of yeast and mammalian cells through RNA-sequencing (RNA-seq) analysis of purified stre...

journal_title：Molecular cell

authors： Khong A,Matheny T,Jain S,Mitchell SF,Wheeler JR,Parker R

更新日期：2017-11-16 00:00:00

abstract：:The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca(2+)] changes ([Ca(2+)](ER-mt)) using drug-inducible fluorescent interorg...

journal_title：Molecular cell

authors： Csordás G,Várnai P,Golenár T,Roy S,Purkins G,Schneider TG,Balla T,Hajnóczky G

更新日期：2010-07-09 00:00:00

abstract：:Metazoan spliceosomes exhibit an elaborate protein composition required for canonical and alternative splicing. Thus, the minimal set of proteins essential for activation and catalysis remains elusive. We therefore purified in vitro assembled, precatalytic spliceosomal complex B, activated B(act), and step 1 complex C...

journal_title：Molecular cell

authors： Fabrizio P,Dannenberg J,Dube P,Kastner B,Stark H,Urlaub H,Lührmann R

更新日期：2009-11-25 00:00:00

abstract：:RanBP2/Nup358 is an essential protein with roles in nuclear transport and mitosis, and is one of the few known SUMO E3 ligases. However, why RanBP2 functions in vivo has been unclear: throughout the cell cycle it stably interacts with RanGAP1*SUMO1 and Ubc9, whose binding sites overlap with the E3 ligase region. Here ...

journal_title：Molecular cell

authors： Werner A,Flotho A,Melchior F

更新日期：2012-05-11 00:00:00

abstract：:Siz1 is a founding member of the Siz/PIAS RING family of SUMO E3 ligases. The X-ray structure of an active Siz1 ligase revealed an elongated tripartite architecture comprised of an N-terminal PINIT domain, a central zinc-containing RING-like SP-RING domain, and a C-terminal domain we term the SP-CTD. Structure-based m...

journal_title：Molecular cell

authors： Yunus AA,Lima CD

更新日期：2009-09-11 00:00:00

abstract：:The PB1-domain-containing proteins p62, aPKC, MEKK2/MEKK3, MEK5, and Par-6 play roles in critical cell processes like osteoclastogenesis, angiogenesis, and early cardiovascular development or cell polarity. PB1 domains are scaffold modules that adopt the topology of ubiquitin-like beta-grasp folds that interact with e...

journal_title：Molecular cell

authors： Moscat J,Diaz-Meco MT,Albert A,Campuzano S

更新日期：2006-09-01 00:00:00

abstract：:In this issue, Roberts et al. (2014) describe how hexokinase and mTORC1 cooperate to sense disequilibrium between glucose uptake and utilization and direct the balance of anabolism and catabolism to ensure the appropriate use of cellular resources. ...

journal_title：Molecular cell

authors： Kundu M

更新日期：2014-02-20 00:00:00

abstract：:The accumulation of unfolded protein in the endoplasmic reticulum (ER) attenuates protein synthesis initiation through phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha) at Ser51. Subsequently, transcription of genes encoding adaptive functions including the glucose-regulate...

journal_title：Molecular cell

authors： Scheuner D,Song B,McEwen E,Liu C,Laybutt R,Gillespie P,Saunders T,Bonner-Weir S,Kaufman RJ

更新日期：2001-06-01 00:00:00

abstract：:SPT5 and its binding partner SPT4 function in both positively and negatively regulating transcriptional elongation. The demonstration that SPT5 and RNA polymerase II are targets for phosphorylation by CDK9/cyclin T1 indicates that posttranslational modifications of these factors are important in regulating the elongat...

journal_title：Molecular cell

authors： Kwak YT,Guo J,Prajapati S,Park KJ,Surabhi RM,Miller B,Gehrig P,Gaynor RB

更新日期：2003-04-01 00:00:00

abstract：:Upon heterologous overexpression, many proteins misfold or aggregate, thus resulting in low functional yields. Human acetylcholinesterase (hAChE), an enzyme mediating synaptic transmission, is a typical case of a human protein that necessitates mammalian systems to obtain functional expression. We developed a computat...

journal_title：Molecular cell

authors： Goldenzweig A,Goldsmith M,Hill SE,Gertman O,Laurino P,Ashani Y,Dym O,Unger T,Albeck S,Prilusky J,Lieberman RL,Aharoni A,Silman I,Sussman JL,Tawfik DS,Fleishman SJ

更新日期：2016-07-21 00:00:00

abstract：:The Wnt3a/β-catenin and Activin/SMAD2,3 signaling pathways synergize to induce endodermal differentiation of human embryonic stem cells; however, the underlying mechanism is not well understood. Using ChIP-seq and GRO-seq analyses, we show here that Wnt3a-induced β-catenin:LEF-1 enhancers recruit cohesin to direct enh...

journal_title：Molecular cell

authors： Estarás C,Benner C,Jones KA

更新日期：2015-06-04 00:00:00

abstract：:To determine whether replicational mutagenesis in the yeast genome is influenced by the positions of active origins, a reporter gene was placed in two orientations at multiple locations within a 39,000 bp region of chromosome III possessing two strong origins. The frequency of mutations resulting from misincorporation...

journal_title：Molecular cell

authors： Pavlov YI,Newlon CS,Kunkel TA

更新日期：2002-07-01 00:00:00