Abstract：
:When inappropriate DNA structures arise, they are sensed by DNA structure-dependent checkpoint pathways and subsequently repaired. Recruitment of checkpoint proteins to such structures precedes recruitment of proteins involved in DNA metabolism. Thus, checkpoints can regulate DNA metabolism. We show that fission yeast Rad9, a 9-1-1 heterotrimeric checkpoint-clamp component, is phosphorylated by Hsk1(Cdc7), the Schizosaccharomyces pombe Dbf4-dependent kinase (DDK) homolog, in response to replication-induced DNA damage. Phosphorylation of Rad9 disrupts its interaction with replication protein A (RPA) and is dependent on 9-1-1 chromatin loading, the Rad9-associated protein Rad4/Cut5(TopBP1), and prior phosphorylation by Rad3(ATR). rad9 mutants defective in DDK phosphorylation show wild-type checkpoint responses but abnormal DNA repair protein foci and decreased viability after replication stress. We propose that Rad9 phosphorylation by DDK releases Rad9 from DNA damage sites to facilitate DNA repair.
journal_name
Mol Celljournal_title
Molecular cellauthors
Furuya K,Miyabe I,Tsutsui Y,Paderi F,Kakusho N,Masai H,Niki H,Carr AMdoi
10.1016/j.molcel.2010.10.026subject
Has Abstractpub_date
2010-11-24 00:00:00pages
606-18issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(10)00831-2journal_volume
40pub_type
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