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Imaging interorganelle contacts and local calcium dynamics at the ER-mitochondrial interface.

Abstract：

:The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca(2+)] changes ([Ca(2+)](ER-mt)) using drug-inducible fluorescent interorganelle linkers. We show that all mitochondria have contacts with the ER, but plasma membrane (PM)-mitochondrial contacts are less frequent because of interleaving ER stacks in both RBL-2H3 and H9c2 cells. Single mitochondria display discrete patches of ER contacts and show heterogeneity in the ER-mitochondrial Ca(2+) transfer. Pericam-tagged linkers revealed IP(3)-induced [Ca(2+)](ER-mt) signals that exceeded 9 microM and endured buffering bulk cytoplasmic [Ca(2+)] increases. Altering linker length to modify the space available for the Ca(2+) transfer machinery had a biphasic effect on [Ca(2+)](ER-mt) signals. These studies provide direct evidence for the existence of high-Ca(2+) microdomains between the ER and mitochondria and suggest an optimal gap width for efficient Ca(2+) transfer.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Csordás G,Várnai P,Golenár T,Roy S,Purkins G,Schneider TG,Balla T,Hajnóczky G

doi

10.1016/j.molcel.2010.06.029

subject

Has Abstract

pub_date

2010-07-09 00:00:00

pages

121-32

issue

1

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(10)00496-X

journal_volume

39

pub_type

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