Abstract：
:Long DNA palindromes are sites of genome instability (deletions, amplification, and translocations) in both prokaryotic and eukaryotic cells. In Escherichia coli, genetic evidence has suggested that they are sites of DNA cleavage by the SbcCD complex that can be repaired by homologous recombination. Here we obtain in vivo physical evidence of an SbcCD-induced DNA double-strand break (DSB) at a palindromic sequence in the E. coli chromosome and show that both ends of the break stimulate recombination. Cleavage is dependent on DNA replication, but the observation of two ends at the break argues that cleavage does not occur at the replication fork. Genetic analysis shows repair of the break requires the RecBCD recombination pathway and PriA, suggesting a mechanism of bacterial DNA DSB repair involving the establishment of replication forks.
journal_name
Mol Celljournal_title
Molecular cellauthors
Eykelenboom JK,Blackwood JK,Okely E,Leach DRdoi
10.1016/j.molcel.2007.12.020subject
Has Abstractpub_date
2008-03-14 00:00:00pages
644-51issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(08)00039-7journal_volume
29pub_type
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