Abstract：
:During transcription initiation by bacterial RNA polymerase, the sigma subunit recognizes the -35 and -10 promoter elements; free sigma, however, does not bind DNA. We selected ssDNA aptamers that strongly and specifically bound free sigma(A) from Thermus aquaticus. A consensus sequence, GTA(C/T)AATGGGA, was required for aptamer binding to sigma(A), with the TA(C/T)AAT segment making interactions similar to those made by the -10 promoter element (consensus sequence TATAAT) in the context of RNA polymerase holoenzyme. When in dsDNA form, the aptamers function as strong promoters for the T. aquaticus RNA polymerase sigma(A) holoenzyme. Recognition of the aptamer-based promoters depends on the downstream GGGA motif from the aptamers' common sequence, which is contacted by sigma(A) region 1.2 and directs transcription initiation even in the absence of the -35 promoter element. Thus, recognition of bacterial promoters is controlled by independent interactions of sigma with multiple basal promoter elements.
journal_name
Mol Celljournal_title
Molecular cellauthors
Feklistov A,Barinova N,Sevostyanova A,Heyduk E,Bass I,Vvedenskaya I,Kuznedelov K,Merkiene E,Stavrovskaya E,Klimasauskas S,Nikiforov V,Heyduk T,Severinov K,Kulbachinskiy Adoi
10.1016/j.molcel.2006.06.010subject
Has Abstractpub_date
2006-07-07 00:00:00pages
97-107issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(06)00417-5journal_volume
23pub_type
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