Abstract：
:Dosage compensation ensures equal expression of X-linked genes in males and females. In Drosophila, equalization is achieved by hypertranscription of the male X chromosome. This process requires an RNA/protein containing dosage compensation complex (DCC). Here we use RNA interference of individual DCC components to define the order of complex assembly in Schneider cells. We show that interaction of MOF with MSL-3 leads to specific acetylation of MSL-3 at a single lysine residue adjacent to one of its chromodomains. We observe that localization of MSL-3 to the X chromosome is RNA dependent and acetylation sensitive. We find that the acetylation status of MSL-3 determines its interaction with roX2 RNA. Furthermore, we find that RPD3 interacts with MSL-3 and that MSL-3 can be deacetylated by the RPD3 complex. We propose that regulated acetylation of MSL-3 may provide a mechanistic explanation for spreading of the dosage compensation complex along the male X chromosome.
journal_name
Mol Celljournal_title
Molecular cellauthors
Buscaino A,Köcher T,Kind JH,Holz H,Taipale M,Wagner K,Wilm M,Akhtar Adoi
10.1016/s1097-2765(03)00140-0subject
Has Abstractpub_date
2003-05-01 00:00:00pages
1265-77issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(03)00140-0journal_volume
11pub_type
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