Abstract：
:DnaA occupies only the three highest-affinity binding sites in E. coli oriC throughout most of the cell cycle. Immediately prior to initiation of chromosome replication, DnaA interacts with additional recognition sites, resulting in localized DNA-strand separation. These two DnaA-oriC complexes formed during the cell cycle are functionally and temporally analogous to yeast ORC and pre-RC. After initiation, SeqA binds to hemimethylated oriC, sequestering oriC while levels of active DnaA are reduced, preventing reinitiation. In this paper, we investigate how resetting of oriC to the ORC-like complex is coordinated with SeqA-mediated sequestration. We report that oriC resets to ORC during sequestration. This was possible because SeqA blocked DnaA binding to hemimethylated oriC only at low-affinity recognition sites associated with GATC but did not interfere with occupation of higher-affinity sites. Thus, during the sequestration period, SeqA repressed pre-RC assembly while ensuring resetting of E. coli ORC.
journal_name
Mol Celljournal_title
Molecular cellauthors
Nievera C,Torgue JJ,Grimwade JE,Leonard ACdoi
10.1016/j.molcel.2006.09.016subject
Has Abstractpub_date
2006-11-17 00:00:00pages
581-92issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(06)00664-2journal_volume
24pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:Mutations in Hnf-1alpha are the most common Mendelian cause of diabetes mellitus. To elucidate the molecular function of a mutational hotspot, we cocrystallized human HNF-1alpha 83-279 with a high-affinity promoter and solved the structure of the complex. Two identical protein molecules are bound to the promoter. Each...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00704-9
更新日期：2002-11-01 00:00:00
abstract：:In this study, we used Saccharomyces cerevisiae to identify a biological network that prevents the deleterious effects of endogenous reactive oxygen species. The absence of Tsa1, a key peroxiredoxin, caused increased rates of mutations, chromosomal rearrangements, and recombination. Defects in recombinational DNA doub...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.02.008
更新日期：2005-03-04 00:00:00
abstract：:Sister-chromatid cohesion describes the orderly association of newly replicated DNA molecules behind replication forks. It plays an essential role in the maintenance and faithful transmission of genetic information. Cohesion is created by DNA topological links and proteinaceous bridges, whose formation and deposition ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.06.033
更新日期：2020-09-03 00:00:00
abstract：:Floral transition in plants is regulated by an integrated network of flowering genetic pathways. We show that an Arabidopsis PIN1-type parvulin 1, Pin1At, controls floral transition by accelerating cis/trans isomerization of the phosphorylated Ser/Thr-Pro motifs in two MADS-domain transcription factors, SUPPRESSOR OF ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.12.020
更新日期：2010-01-15 00:00:00
abstract：:MicroRNAs (miRNAs) play a pivotal role in the regulation of genes involved in diverse processes such as development, differentiation, and cellular growth control. Recently, many viral-encoded miRNAs have been discovered, for the most part in viruses transcribed from double-stranded DNA genomes. As with their cellular ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2005.09.012
更新日期：2005-10-07 00:00:00
abstract：:The phosphatidylinositol 3-kinase (PI3K) signaling pathway is frequently deregulated in cancer. Downstream of PI3K, Akt1 and Akt2 have opposing roles in breast cancer invasive migration, leading to metastatic dissemination. Here, we identify palladin, an actin-associated protein, as an Akt1-specific substrate that mod...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.02.031
更新日期：2010-05-14 00:00:00
abstract：:Melanoma and other cancers harbor oncogenic mutations in the protein kinase B-Raf, which leads to constitutive activation and dysregulation of MAP kinase signaling. In order to elucidate molecular determinants responsible for B-Raf control of cancer phenotypes, we present a method for phosphoprotein profiling, using n...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.03.007
更新日期：2009-04-10 00:00:00
abstract：:In the current issue of Molecular Cell, Liu et al. (2020) show that the secretion of cancer-linked forms of mutant calreticulin allow cancer cells to escape protective immune responses induced by chemotherapeutic and immunotherapeutic drugs, thereby promoting tumor growth. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2020.01.024
更新日期：2020-02-20 00:00:00
abstract：:The genomes of mammalian neurons contain uniquely high levels of non-CG DNA methylation that can be bound by the Rett syndrome protein, MeCP2, to regulate gene expression. How patterns of non-CG methylation are established in neurons and the mechanism by which this methylation works with MeCP2 to control gene expressi...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.10.033
更新日期：2020-01-16 00:00:00
abstract：:APOBEC1 is the catalytic component of an RNA editing complex but shows homology to activation-induced cytidine deaminase (AID), a protein whose function is to potentiate diversification of immunoglobulin gene DNA. Here, we show that APOBEC1 and its homologs APOBEC3C and APOBEC3G exhibit potent DNA mutator activity in ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00742-6
更新日期：2002-11-01 00:00:00
abstract：:Cryo-EM structures of Dot1L in complex with a ubiquitinated nucleosome provide the long-sought-after molecular mechanism of Dot1L-mediated methylation of lysine 79 in histone H3 and explain crosstalk with histone H2B ubiquitination. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2019.05.013
更新日期：2019-06-06 00:00:00
abstract：:Posttranslational modification of proliferating cell nuclear antigen (PCNA), an essential processivity clamp for DNA polymerases, by ubiquitin and SUMO contributes to the coordination of DNA replication, damage tolerance, and mutagenesis. Whereas ubiquitination in response to DNA damage promotes the bypass of replicat...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.06.001
更新日期：2005-07-01 00:00:00
abstract：:In a recent issue of Molecular Cell, Kretov et al. (2020) demonstrate that microRNA-144 targets Dicer in a negative feedback loop, affecting global canonical microRNA expression in erythrocytes. MicroRNA-451 is refractory to the loss of Dicer because of its Ago2-dependent processing. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2020.04.028
更新日期：2020-06-04 00:00:00
abstract：:Examination of the binding of FeBABE-conjugated BvgA to the fha promoter of Bordetella pertussis has revealed that three dimers, formed by head-to-head association of monomers, bind one face of the DNA helix from the inverted-heptad primary binding site to the -35 region. The orientation of BvgA monomers within the di...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00007-8
更新日期：2003-01-01 00:00:00
abstract：:In this issue, Roberts et al. (2014) describe how hexokinase and mTORC1 cooperate to sense disequilibrium between glucose uptake and utilization and direct the balance of anabolism and catabolism to ensure the appropriate use of cellular resources. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2014.02.009
更新日期：2014-02-20 00:00:00
abstract：:Epitranscriptomic marks are dynamically placed on mRNA by "writer" or "eraser" enzymes, while "readers" modulate their function accordingly. Lin et al. (2016) now report that the N6-methyladenosine:RNA methyltransferase METTL3 is both a writer and a reader, directly enhancing mRNA translation. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2016.04.024
更新日期：2016-05-05 00:00:00
abstract：:miRNAs are largely known to base pair with the 3'UTR of target mRNAs, downregulating their stability and translation. mRNA of betaTrCP1 ubiquitin ligase is very unstable, but unlike the majority of mRNAs where 3'UTR determines the rate of mRNA turnover, betaTrCP1 mRNA contains cis-acting destabilizing elements within ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.06.007
更新日期：2009-07-31 00:00:00
abstract：:RNA polymerases (RNAPs) are core components of the cellular transcriptional machinery. Progress with functional studies of eukaryotic RNAPs has been delayed by the fact that it has not yet been possible to assemble active enzymes from individual subunits. Archaeal RNAPs are directly comparable to eukaryotic RNAPII in ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00629-9
更新日期：2002-09-01 00:00:00
abstract：:The Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how the Polycomb system selects its target genes is poorly understood, and whether its histone-modifying activities are required for transcriptional repression remains controversial. Here, we...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.12.001
更新日期：2020-02-20 00:00:00
abstract：:Prokaryotes use a mechanism called priming to update their CRISPR immunological memory to rapidly counter revisiting, mutated viruses, and plasmids. Here we have determined how new spacers are produced and selected for integration into the CRISPR array during priming. We show that Cas3 couples CRISPR interference to a...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.07.011
更新日期：2016-09-01 00:00:00
abstract：:Eukaryotic gene expression regulation involves thousands of distal regulatory elements. Understanding the quantitative contribution of individual enhancers to gene expression is critical for assessing the role of disease-associated genetic risk variants. Yet, we lack the ability to accurately link genes with their dis...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.09.005
更新日期：2020-10-15 00:00:00
abstract：:The anaphase-promoting complex (APC) is a ubiquitin ligase that promotes the degradation of cell-cycle regulators. Cdh1p is an APC coactivator that directly binds APC substrates. A genetic screen in budding yeast identified residues within Cdh1p critical for its function. Cdh1p proteins containing mutations within the...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.04.022
更新日期：2005-05-27 00:00:00
abstract：:Initiation sites for DNA synthesis in the chromosomal autonomously replicating sequence (ARS)1 of Saccharomyces cerevisiae were detected at the nucleotide level. The transition from discontinuous to continuous synthesis defines the origin of bidirectional replication (OBR), which mapped adjacent to the origin recognit...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80475-x
更新日期：1999-04-01 00:00:00
abstract：:There are many regulated steps in the assembly of a transcription preinitiation complex (PIC). In this issue of Molecular Cell, Black et. al. (2006) reveal a catalytic switch mechanism in which autoacetylation of the HAT p300 triggers its dissociation from a promoter in a manner that is coupled to TFIID association. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.molcel.2006.08.022
更新日期：2006-09-15 00:00:00
abstract：:SOCS3 is essential for regulating the extent, duration, and specificity of cellular responses to cytokines such as G-CSF and IL-6. Here we describe the solution structure of SOCS3, the first structure determined for any SOCS protein, in complex with a phosphotyrosine-containing peptide from the IL-6 receptor signaling...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.03.024
更新日期：2006-04-21 00:00:00
abstract：:Members of the ISWI family of chromatin remodeling factors exhibit ATP-dependent nucleosome sliding, loading, and spacing activities in vitro. However, it is unclear which of these activities are utilized by ISWI complexes to remodel chromatin in vivo. We therefore sought to identify the mechanisms of chromatin remode...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00436-2
更新日期：2003-11-01 00:00:00
abstract：:Homologous recombination (HR) is crucial for genetic exchange and accurate repair of DNA double-strand breaks and is pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly und...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2013.02.020
更新日期：2013-04-25 00:00:00
abstract：:Nucleosome loss from a promoter region has recently been described as a potential mechanism for transcriptional regulation. We investigated whether H3/H4 histone chaperones mediate the loss of nucleosomes from the promoter of the yeast PHO5 gene during transcriptional activation. We found that antisilencing function 1...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2004.05.016
更新日期：2004-06-04 00:00:00
abstract：:Mammalian lefty and zebrafish antivin form a subgroup of the TGF beta superfamily. We report that mouse mutants for lefty2 have an expanded primitive streak and form excess mesoderm, a phenotype opposite to that of mutants for the TGF beta gene nodal. Analogously, overexpression of Antivin or Lefty2 in zebrafish embry...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80331-7
更新日期：1999-09-01 00:00:00
abstract：:Prions constitute a rare class of protein, which can switch to a robust amyloid form and then propagate that form in the absence of a nucleic acid determinant, thereby creating a unique, protein-only infectious agent. Details of the mechanism that drives conversion to the prion form and then subsequent propagation of ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2004.05.012
更新日期：2004-06-04 00:00:00
