Abstract：
:The stimulation of fibroblast growth factor receptors (FGFRs) with distinct FGF ligands generates specific cellular responses. However, the mechanisms underlying this paradigm have remained elusive. Here, we show that FGF-7 stimulation leads to FGFR2b degradation and, ultimately, cell proliferation, whereas FGF-10 promotes receptor recycling and cell migration. By combining mass-spectrometry-based quantitative proteomics with fluorescence microscopy and biochemical methods, we find that FGF-10 specifically induces the rapid phosphorylation of tyrosine (Y) 734 on FGFR2b, which leads to PI3K and SH3BP4 recruitment. This complex is crucial for FGFR2b recycling and responses, given that FGF-10 stimulation of either FGFR2b_Y734F mutant- or SH3BP4-depleted cells switches the receptor endocytic route to degradation, resulting in decreased breast cancer cell migration and the inhibition of epithelial branching in mouse lung explants. Altogether, these results identify an intriguing ligand-dependent mechanism for the control of receptor fate and cellular outputs that may explain the pathogenic role of deregulated FGFR2b, thus offering therapeutic opportunities.
journal_name
Mol Celljournal_title
Molecular cellauthors
Francavilla C,Rigbolt KT,Emdal KB,Carraro G,Vernet E,Bekker-Jensen DB,Streicher W,Wikström M,Sundström M,Bellusci S,Cavallaro U,Blagoev B,Olsen JVdoi
10.1016/j.molcel.2013.08.002subject
Has Abstractpub_date
2013-09-26 00:00:00pages
707-22issue
6eissn
1097-2765issn
1097-4164pii
S1097-2765(13)00575-3journal_volume
51pub_type
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