Abstract:
:The histone methyltransferase PR-Set7/Set8 is the sole enzyme that catalyzes monomethylation of histone H4 at K20 (H4K20me1). Previous reports document disparate evidence regarding PR-Set7 expression during the cell cycle, the biological relevance of PR-Set7 interaction with PCNA, and its role in the cell. We find that PR-Set7 is indeed undetectable during S phase and instead is detected during late G2, mitosis, and early G1. PR-Set7 is transiently recruited to laser-induced DNA damage sites through its interaction with PCNA, after which 53BP1 is recruited dependent on PR-Set7 catalytic activity. During the DNA damage response, PR-Set7 interaction with PCNA through a specialized "PIP degron" domain targets it for PCNA-coupled CRL4(Cdt2)-dependent proteolysis. PR-Set7 mutant in its "PIP degron" is now detectable during S phase, during which the mutant protein accumulates. Outside the chromatin context, Skp2 promotes PR-Set7 degradation as well. These findings demonstrate a stringent spatiotemporal control of PR-Set7 that is essential for preserving the genomic integrity of mammalian cells.
journal_name
Mol Celljournal_title
Molecular cellauthors
Oda H,Hübner MR,Beck DB,Vermeulen M,Hurwitz J,Spector DL,Reinberg Ddoi
10.1016/j.molcel.2010.10.011subject
Has Abstractpub_date
2010-11-12 00:00:00pages
364-76issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(10)00787-2journal_volume
40pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract::Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient recycling, and various aspects of growth and development in eukaryotes. Here we show that autophagy impacts the other major degradative route involving the ubiquitin-proteasome system by eliminating 26S proteasomes, a pro...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.04.023
更新日期:2015-06-18 00:00:00
abstract::Gene expression mediated by viral vectors is subject to cell-to-cell variability, which limits the accuracy of gene delivery. When coupled with single-cell measurements, however, such variability provides an efficient means to quantify signaling dynamics in mammalian cells. Here, we illustrate the utility of this appr...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.01.014
更新日期:2011-02-04 00:00:00
abstract::The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to the unique aspects of their promoter structure, selectivity for either RNA polymerase (Pol) II or III, and because of their unique mechanism of termination that is tightly linked with the pro...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2013.07.003
更新日期:2013-08-22 00:00:00
abstract::Integration of the mycobacteriophage Bxb1 genome into its host chromosome is catalyzed by a serine-integrase, a member of the transposon-resolvase family of site-specific recombinases. These enzymes use a concerted mechanism of strand exchange involving double-stranded cleavages with two-base extensions, and covalent ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00444-1
更新日期:2003-11-01 00:00:00
abstract::RNA editing in the mitochondria of kinetoplastids involves the addition and deletion of uridines at specific sites as directed by guide RNAs (gRNAs). Ample evidence shows that ribonucleoprotein (RNP) complexes carry out this posttranscriptional processing. One component of RNA editing complexes is REAP-1, a protein of...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00231-3
更新日期:2001-04-01 00:00:00
abstract::We used computational algorithms to find conserved sequences in the 3' untranslated region (UTR) of transcripts that exhibited rapid decay in primary human T cells and found that the consensus sequence UGUUUGUUUGU, which we have termed a GU-rich element (GRE), was enriched in short-lived transcripts. Using a tet-off r...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.11.024
更新日期:2008-02-01 00:00:00
abstract::Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.02.011
更新日期:2009-03-27 00:00:00
abstract::Nup98 is a component of the nuclear pore that plays its primary role in the export of RNAs. Nup98 is expressed in two forms, derived from alternate mRNA splicing. Both forms are processed into two peptides through autoproteolysis mediated by the C-terminal domain of hNup98. The three-dimensional structure of the C-ter...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00589-0
更新日期:2002-08-01 00:00:00
abstract::Mismatch repair (MMR) is initiated by MutS family proteins (MSH) that recognize DNA mismatches and recruit downstream repair factors. We used a single-molecule DNA-unzipping assay to probe interactions between S. cerevisiae MSH2-MSH6 and a variety of DNA mismatch substrates. This work revealed a high-specificity bindi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.10.014
更新日期:2005-12-09 00:00:00
abstract::Mitochondrial protein import stress compromises functioning of the organelles, due to inadequate supply of inner mitochondrial proteins. Weidberg and Amon (2018) describe a new monitoring pathway in budding yeast, which restores mitochondrial function following the clearing of accumulated unfolded pre-transported mito...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2018.06.027
更新日期:2018-07-05 00:00:00
abstract::Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA polymerase II (RNAPII) at nucleotide resolution. We find that the entry site of the first ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2014.02.014
更新日期:2014-03-06 00:00:00
abstract::Growth factors such as epidermal growth factor (EGF) and insulin regulate development and metabolism via genes containing both POU homeodomain (Pit-1) and phorbol ester (AP-1) response elements. Although CREB binding protein (CBP) functions as a coactivator on these elements, the mechanism of transactivation was previ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00202-7
更新日期:2001-03-01 00:00:00
abstract::The protein kinase B-RAF is mutated in approximately 7% of human cancers. Most mutations are activating, but, surprisingly, a small number have reduced kinase activity. However, the latter can still stimulate cellular signaling through the MEK-ERK pathway because they activate the related family member C-RAF. We exami...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.10.022
更新日期:2005-12-22 00:00:00
abstract::Epigenetic silencing of transposons by Piwi-interacting RNAs (piRNAs) constitutes an RNA-based genome defense mechanism. Piwi endonuclease action amplifies the piRNA pool by generating new piRNAs from target transcripts by a poorly understood mechanism. Here, we identified mouse Fkbp6 as a factor in this biogenesis pa...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2012.07.019
更新日期:2012-09-28 00:00:00
abstract::Poly-ubiquitin chains direct protein substrates to the 26S proteasome, where they are removed by the deubiquitinase Rpn11 during ATP-dependent substrate degradation. Rapid deubiquitination is required for efficient degradation but must be restricted to committed substrates that are engaged with the ATPase motor to pre...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.07.023
更新日期:2017-09-07 00:00:00
abstract::Organisms must survive a variety of stressful conditions, including sudden temperature increases that damage important cellular structures and interfere with essential functions. In response to heat stress, cells activate an ancient signaling pathway leading to the transient expression of heat shock or heat stress pro...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2010.10.006
更新日期:2010-10-22 00:00:00
abstract::Allosteric communication between interacting molecules is fundamental to signal transduction and many other cellular processes. To better understand the relationship between nuclear receptor (NR) ligand positioning and the formation of the coactivator binding pocket, we investigated the determinants of ligand selectiv...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00054-1
更新日期:2004-02-13 00:00:00
abstract::Maintaining a fluid bilayer is essential for cell signaling and survival. Lipid saturation is a key factor determining lipid packing and membrane fluidity, and it must be tightly controlled to guarantee organelle function and identity. A dedicated eukaryotic mechanism of lipid saturation sensing, however, remains elus...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.05.015
更新日期:2016-07-07 00:00:00
abstract::During meiosis, recombination between homologous chromosomes generates crossover (CR) and noncrossover (NCR) products. CRs establish connections between homologs, whereas intermediates leading to NCRs have been proposed to participate in homologous pairing. How these events are differentiated and regulated remains to ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.09.021
更新日期:2005-11-23 00:00:00
abstract::The dynamic protein interactions required for transcription are functionally important yet poorly understood; in this issue of Molecular Cell, Zobeck et al. (2010) resolve the sequential recruitment and selective recycling of transcription factors at an actively transcribing locus in Drosophila. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2010.12.010
更新日期:2010-12-22 00:00:00
abstract::Polycomb proteins maintain cell identity by repressing the expression of developmental regulators specific for other cell types. Polycomb repressive complex-2 (PRC2) catalyzes trimethylation of histone H3 lysine-27 (H3K27me3). Although repressed, PRC2 targets are generally associated with the transcriptional initiatio...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2010.03.019
更新日期:2010-06-11 00:00:00
abstract::BRAF is an oncogenic protein kinase that drives cell growth and proliferation through the MEK-ERK signaling pathway. BRAF inhibitors have demonstrated antitumor efficacy in melanoma therapy but have also been found to be associated with the development of cutaneous squamous cell carcinomas (cSCCs) in certain patients....
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2013.08.044
更新日期:2013-10-24 00:00:00
abstract::Dbh is a Y family translesion DNA polymerase that accurately bypasses some damaged forms of deoxyguanosine, but also generates single-base deletion errors at frequencies of up to 50%, in specific hot spot sequences. We describe preinsertion binary, insertion ternary, and postinsertion binary crystal structures of Dbh ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2008.01.014
更新日期:2008-03-28 00:00:00
abstract::The interaction between pRB and E2F is critical for control of the cell cycle and apoptosis. Here we report that pRB contains two distinct E2F binding sites. The previously identified E2F binding site on pRB is necessary for stable association with E2Fs on DNA. A second E2F interaction site is located entirely within ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00344-7
更新日期:2003-09-01 00:00:00
abstract::The steroid hormone ecdysone signals the stage-specific programmed cell death of the larval salivary glands during Drosophila metamorphosis. This response is preceded by an ecdysone-triggered switch in gene expression in which the diap2 death inhibitor is repressed and the reaper (rpr) and head involution defective (h...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80439-6
更新日期:2000-03-01 00:00:00
abstract::While much research has examined the use of glucose and glutamine by tumor cells, many cancers instead prefer to metabolize fats. Despite the pervasiveness of this phenotype, knowledge of pathways that drive fatty acid oxidation (FAO) in cancer is limited. Prolyl hydroxylase domain proteins hydroxylate substrate proli...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.08.014
更新日期:2016-09-15 00:00:00
abstract::The WTX gene is frequently lost or mutated in Wilms tumor. In this issue of Molecular Cell, Kim et al. (2012) identify WTX modulation of p53 tumor-suppressor activity through regulation of p53 acetylation. Therefore, WTX differentially regulates the oncogenic β-catenin pathway and the tumor-suppressing p53 pathway. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2012.02.010
更新日期:2012-03-09 00:00:00
abstract::During X-inactivation, Xist RNA spreads along an entire chromosome to establish silencing. However, the mechanism and functional RNA elements involved in spreading remain undefined. By performing a comprehensive endogenous Xist deletion screen, we identify Repeat B as crucial for spreading Xist and maintaining Polycom...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2019.01.015
更新日期:2019-04-04 00:00:00
abstract::Curli are extracellular functional amyloids that are assembled by enteric bacteria during biofilm formation and host colonization. An efficient secretion system and chaperone network ensures that the major curli fiber subunit, CsgA, does not form intracellular amyloid aggregates. We discovered that the periplasmic pro...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2014.12.025
更新日期:2015-02-05 00:00:00
abstract::Inappropriate activation of oncogenic kinases at intracellular locations is frequently observed in human cancers, but its effects on global signaling are incompletely understood. Here, we show that the oncogenic mutant of Flt3 (Flt3-ITD), when localized at the endoplasmic reticulum (ER), aberrantly activates STAT5 and...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.09.019
更新日期:2009-10-23 00:00:00