Abstract:
:Polycomb proteins maintain cell identity by repressing the expression of developmental regulators specific for other cell types. Polycomb repressive complex-2 (PRC2) catalyzes trimethylation of histone H3 lysine-27 (H3K27me3). Although repressed, PRC2 targets are generally associated with the transcriptional initiation marker H3K4me3, but the significance of this remains unclear. Here, we identify a class of short RNAs, approximately 50-200 nucleotides in length, transcribed from the 5' end of polycomb target genes in primary T cells and embryonic stem cells. Short RNA transcription is associated with RNA polymerase II and H3K4me3, occurs in the absence of mRNA transcription, and is independent of polycomb activity. Short RNAs form stem-loop structures resembling PRC2 binding sites in Xist, interact with PRC2 through SUZ12, cause gene repression in cis, and are depleted from polycomb target genes activated during cell differentiation. We propose that short RNAs play a role in the association of PRC2 with its target genes.
journal_name
Mol Celljournal_title
Molecular cellauthors
Kanhere A,Viiri K,Araújo CC,Rasaiyaah J,Bouwman RD,Whyte WA,Pereira CF,Brookes E,Walker K,Bell GW,Pombo A,Fisher AG,Young RA,Jenner RGdoi
10.1016/j.molcel.2010.03.019subject
Has Abstractpub_date
2010-06-11 00:00:00pages
675-88issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(10)00327-8journal_volume
38pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract::Mammalian lefty and zebrafish antivin form a subgroup of the TGF beta superfamily. We report that mouse mutants for lefty2 have an expanded primitive streak and form excess mesoderm, a phenotype opposite to that of mutants for the TGF beta gene nodal. Analogously, overexpression of Antivin or Lefty2 in zebrafish embry...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80331-7
更新日期:1999-09-01 00:00:00
abstract::In this issue of Molecular Cell, Fleming et al. (2008) show that histone H2B ubiquitylation and FACT function interdependently to facilitate nucleosome reassembly during transcription elongation, thereby demonstrating that histone posttranslational modifications can provide important but transient transcriptional sign...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2008.06.012
更新日期:2008-07-11 00:00:00
abstract::A major unresolved question in messenger RNA translation is how ribosomal release factors terminate protein synthesis. Class 1 release factors decode stop codons and trigger hydrolysis of the bond between the nascent polypeptide and tRNA some 75 A away from the decoding site. While the gross features of the release fa...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.06.032
更新日期:2007-09-07 00:00:00
abstract::Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor e...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80122-7
更新日期:1998-07-01 00:00:00
abstract::Tet(O) belongs to a class of ribosomal protection proteins that mediate tetracycline resistance. It is a G protein that shows significant sequence similarity to elongation factor EF-G. Here we present a cryo-electron microscopic reconstruction, at 16 A resolution, of its complex with the E. coli 70S ribosome. Tet(O) w...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00238-6
更新日期:2001-05-01 00:00:00
abstract::In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied ini...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.08.011
更新日期:2016-09-15 00:00:00
abstract::Glaucoma, a blinding neurodegenerative disease, whose risk factors include elevated intraocular pressure (IOP), age, and genetics, is characterized by accelerated and progressive retinal ganglion cell (RGC) death. Despite decades of research, the mechanism of RGC death in glaucoma is still unknown. Here, we demonstrat...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.07.027
更新日期:2015-09-17 00:00:00
abstract::In a recent issue of Molecular Cell, Kretov et al. (2020) demonstrate that microRNA-144 targets Dicer in a negative feedback loop, affecting global canonical microRNA expression in erythrocytes. MicroRNA-451 is refractory to the loss of Dicer because of its Ago2-dependent processing. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2020.04.028
更新日期:2020-06-04 00:00:00
abstract::Using Micro-C, Hsieh et al. (2020) and Krietenstein et al. (2020) investigated 3D chromatin folding in human and mouse cells at unprecedented resolution to uncover ultra-fine-scale chromatin structures that provide direct links between genome architecture, gene expression, and gene regulation. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2020.04.021
更新日期:2020-05-07 00:00:00
abstract::Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartm...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2019.05.001
更新日期:2019-07-25 00:00:00
abstract::The presence of microbial or self DNA in the cytoplasm of mammalian cells is a danger signal detected by the DNA sensor cyclic-GMP-AMP (cGAMP) synthase (cGAS), which catalyzes the production of cGAMP that in turn serves as a second messenger to activate innate immune responses. Here we show that endogenous cGAMP in ma...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2013.05.022
更新日期:2013-07-25 00:00:00
abstract::Distinctive from their normal counterparts, cancer cells exhibit unique metabolic dependencies on glutamine to fuel anabolic processes. Specifically, pancreatic ductal adenocarcinoma (PDAC) cells rely on an unconventional metabolic pathway catalyzed by aspartate aminotransferase, malate dehydrogenase 1 (MDH1), and mal...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.09.028
更新日期:2016-11-17 00:00:00
abstract::We used DNA microarrays to profile gene expression patterns in the C. elegans germline and identified 1416 germline-enriched transcripts that define three groups. The sperm-enriched group contains an unusually large number of protein kinases and phosphatases. The oocyte-enriched group includes potentially new componen...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00059-9
更新日期:2000-09-01 00:00:00
abstract::Activation of retrograde signaling (RS) by mitochondrial dysfunction or by inhibition of TOR kinases in yeast results in nuclear accumulation of the transcription factors, Rtg1p and Rtg3p. This process requires Rtg2p, a novel cytoplasmic protein with an N-terminal ATP binding domain. We show that Rtg2p controls RS by ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00285-5
更新日期:2003-08-01 00:00:00
abstract::The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor contro...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.08.013
更新日期:2005-10-07 00:00:00
abstract::Bcr-Abl is a dysregulated tyrosine kinase whose mechanism of activation is unclear. Here, we demonstrate that, like c-Abl, Bcr-Abl is negatively regulated through its SH3 domain. Kinase activity, transformation, and leukemogenesis by Bcr-Abl are greatly impaired by mutations of the Bcr coiled-coil domain that disrupt ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00274-0
更新日期:2003-07-01 00:00:00
abstract::Tumor progression shares many characteristics with the process of epithelial-to-mesenchymal transition (EMT). Cells that have undergone an EMT are known to have an increased resistance to apoptosis. CD95/Fas is an apoptosis-inducing receptor expressed on many tissues and tumor cells. During tumor progression CD95 is f...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2010.05.018
更新日期:2010-06-25 00:00:00
abstract::Cells constantly adjust their metabolism in response to environmental conditions, yet major mechanisms underlying survival remain poorly understood. We discover a posttranscriptional mechanism that integrates starvation response with GTP homeostasis to allow survival, enacted by the nucleotide (p)ppGpp, a key player i...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2012.08.009
更新日期:2012-10-26 00:00:00
abstract::The phosphatidylinositol 3-kinase (PI3K) signaling pathway is frequently deregulated in cancer. Downstream of PI3K, Akt1 and Akt2 have opposing roles in breast cancer invasive migration, leading to metastatic dissemination. Here, we identify palladin, an actin-associated protein, as an Akt1-specific substrate that mod...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2010.02.031
更新日期:2010-05-14 00:00:00
abstract::In the death receptor induced apoptotic pathway, caspase-8 autocatalytically cleaves itself at specific cleavage sites. To better understand the regulatory mechanisms behind caspase-8 activation, we compared active wild-type caspase-8 (wtC8) and an uncleavable form of procaspase-8 (uncleavable C8). We demonstrate that...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00059-5
更新日期:2003-02-01 00:00:00
abstract::YAP/TEAD are nuclear effectors of the Hippo pathway, regulating organ size and tumorigenesis largely through promoter-associated function. However, their function as enhancer regulators remains poorly understood. Through an in vivo proximity-dependent labeling (BioID) technique, we identified YAP1 and TEAD4 protein as...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2019.06.010
更新日期:2019-08-22 00:00:00
abstract::Epitranscriptomic marks are dynamically placed on mRNA by "writer" or "eraser" enzymes, while "readers" modulate their function accordingly. Lin et al. (2016) now report that the N6-methyladenosine:RNA methyltransferase METTL3 is both a writer and a reader, directly enhancing mRNA translation. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2016.04.024
更新日期:2016-05-05 00:00:00
abstract::tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.11.014
更新日期:2007-01-12 00:00:00
abstract::Sister-chromatid cohesion describes the orderly association of newly replicated DNA molecules behind replication forks. It plays an essential role in the maintenance and faithful transmission of genetic information. Cohesion is created by DNA topological links and proteinaceous bridges, whose formation and deposition ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2020.06.033
更新日期:2020-09-03 00:00:00
abstract::Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA polymerase II (RNAPII) at nucleotide resolution. We find that the entry site of the first ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2014.02.014
更新日期:2014-03-06 00:00:00
abstract::The bHLH factors HAND1 and HAND2 are required for heart, vascular, neuronal, limb, and extraembryonic development. Unlike most bHLH proteins, HAND factors exhibit promiscuous dimerization properties. We report that phosphorylation/dephosphorylation via PKA, PKC, and a specific heterotrimeric protein phosphatase 2A (PP...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00425-8
更新日期:2003-11-01 00:00:00
abstract::R loop, a transcription intermediate containing RNA:DNA hybrids and displaced single-stranded DNA (ssDNA), has emerged as a major source of genomic instability. RNaseH1, which cleaves the RNA in RNA:DNA hybrids, plays an important role in R loop suppression. Here we show that replication protein A (RPA), an ssDNA-bind...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.01.029
更新日期:2017-03-02 00:00:00
abstract::The RhoA GTPase controls many cellular functions, including gene transcription and actin polymerization. Several lines of evidence suggest that Rho GTPases are required for B cell receptor (BCR) signaling, but whether RhoA is necessary has not been investigated. Here, we show that RhoA is activated, downstream of PI3K...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.12.012
更新日期:2005-01-21 00:00:00
abstract::AAA ATPases play central roles in cellular activities. The ATPase p97, a prototype of this superfamily, participates in organelle membrane fusion. Cryoelectron microscopy and single-particle analysis revealed that a major conformational change of p97 during the ATPase cycle occurred upon nucleotide binding and not dur...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00144-1
更新日期:2000-12-01 00:00:00
abstract::p73 has been identified as a structural and functional homolog of the tumor suppressor p53. The transcriptional coactivator Yes-associated protein (YAP) has been demonstrated to interact with and to enhance p73-dependent apoptosis in response to DNA damage. Here, we show the existence of a proapoptotic autoregulatory ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2008.11.019
更新日期:2008-12-26 00:00:00