Abstract:
:Activation of retrograde signaling (RS) by mitochondrial dysfunction or by inhibition of TOR kinases in yeast results in nuclear accumulation of the transcription factors, Rtg1p and Rtg3p. This process requires Rtg2p, a novel cytoplasmic protein with an N-terminal ATP binding domain. We show that Rtg2p controls RS by reversibly binding a negative regulator, Mks1p. The inhibitory form of Mks1p is phosphorylated and complexed with the 14-3-3 proteins, Bmh1p and Bmh2p, which are also negative regulators of RS. A hypophosphorylated form of Mks1p bound to Rtg2p is inactive. Point mutations in the Rtg2p ATP binding domain simultaneously block RS and Mks1p-Rtg2p interaction. We propose that activation of RS via mitochondrial dysfunction and TOR inhibition intersect at the Rtg2p-Mks1p switch.
journal_name
Mol Celljournal_title
Molecular cellauthors
Liu Z,Sekito T,Spírek M,Thornton J,Butow RAdoi
10.1016/s1097-2765(03)00285-5subject
Has Abstractpub_date
2003-08-01 00:00:00pages
401-11issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(03)00285-5journal_volume
12pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract::In the current issue of Molecular Cell, Liu et al. (2020) show that the secretion of cancer-linked forms of mutant calreticulin allow cancer cells to escape protective immune responses induced by chemotherapeutic and immunotherapeutic drugs, thereby promoting tumor growth. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2020.01.024
更新日期:2020-02-20 00:00:00
abstract::Our previous study showed that ubiquitination of p53 is reversible and that the ubiquitin hydrolase HAUSP can stabilize p53 by deubiquitination. Here, we found that partial reduction of endogenous HAUSP levels by RNAi indeed destabilizes endogenous p53; surprisingly, however, nearly complete ablation of HAUSP stabiliz...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00157-1
更新日期:2004-03-26 00:00:00
abstract::Telomeres protect chromosome ends from fusing to double-stranded breaks (DSBs). Using a quantitative real-time PCR assay, we show that nonhomologous end joining between a telomere and an inducible DSB was undetectable in wild-type cells, but occurred within a few hours of DSB induction in approximately 1/2000 genomes ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00174-6
更新日期:2003-05-01 00:00:00
abstract::Maintaining a fluid bilayer is essential for cell signaling and survival. Lipid saturation is a key factor determining lipid packing and membrane fluidity, and it must be tightly controlled to guarantee organelle function and identity. A dedicated eukaryotic mechanism of lipid saturation sensing, however, remains elus...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.05.015
更新日期:2016-07-07 00:00:00
abstract::Ubiquitin modification of proteins plays pivotal roles in the cellular response to DNA damage. Given the complexity of ubiquitin conjugation due to the formation of poly-conjugates of different linkages, functional roles of linkage-specific ubiquitin modification at DNA damage sites are largely unclear. We identify th...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.04.013
更新日期:2017-05-18 00:00:00
abstract::Regulation of gene expression by stress-activated protein kinases (SAPKs) is essential for cell adaptation to extracellular stimuli. Exposure of yeast to high osmolarity results in activation of the SAPK Hog1, which associates with transcription factors bound at target promoters and stimulates transcriptional initiati...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.05.031
更新日期:2006-07-21 00:00:00
abstract::The proper processing, export, localization, translation, and degradation of mRNAs are necessary for regulation of gene expression. These processes are controlled by mRNA-specific regulatory proteins, noncoding RNAs, and core machineries common to most mRNAs. These factors bind the mRNA in large complexes known as mes...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2014.04.033
更新日期:2014-05-22 00:00:00
abstract::Glaucoma, a blinding neurodegenerative disease, whose risk factors include elevated intraocular pressure (IOP), age, and genetics, is characterized by accelerated and progressive retinal ganglion cell (RGC) death. Despite decades of research, the mechanism of RGC death in glaucoma is still unknown. Here, we demonstrat...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.07.027
更新日期:2015-09-17 00:00:00
abstract::CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.12.015
更新日期:2019-02-21 00:00:00
abstract::Elongation factor G (EF-G) catalyzes tRNA translocation on the ribosome. Here a cryo-EM reconstruction of the 70S*EF-G ribosomal complex at 7.3 A resolution and the crystal structure of EF-G-2*GTP, an EF-G homolog, at 2.2 A resolution are presented. EF-G-2*GTP is structurally distinct from previous EF-G structures, an...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.01.027
更新日期:2007-03-09 00:00:00
abstract::The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca(2+)] changes ([Ca(2+)](ER-mt)) using drug-inducible fluorescent interorg...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2010.06.029
更新日期:2010-07-09 00:00:00
abstract::Protein kinase RNA-activated (PKR) induces immune response by sensing viral double-stranded RNAs (dsRNAs). However, growing evidence suggests that PKR can also be activated by endogenously expressed dsRNAs. Here, we capture these dsRNAs by formaldehyde-mediated crosslinking and immunoprecipitation sequencing and find ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.07.029
更新日期:2018-09-20 00:00:00
abstract::The convergence of two DNA replication forks creates unique problems during DNA replication termination. In E. coli and SV40, the release of torsional strain by type II topoisomerases is critical for converging replisomes to complete DNA synthesis, but the pathways that mediate fork convergence in eukaryotes are unkno...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2019.01.040
更新日期:2019-04-18 00:00:00
abstract::MicroRNAs (miRNAs) play a pivotal role in the regulation of genes involved in diverse processes such as development, differentiation, and cellular growth control. Recently, many viral-encoded miRNAs have been discovered, for the most part in viruses transcribed from double-stranded DNA genomes. As with their cellular ...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2005.09.012
更新日期:2005-10-07 00:00:00
abstract::Protein phase separation by low-complexity, intrinsically disordered domains generates membraneless organelles and links to neurodegeneration. Cellular prion protein (PrPC) contains such domains, causes spongiform degeneration, and is a receptor for Alzheimer's amyloid-β oligomers (Aβo). Here, we show that PrPC separa...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.10.009
更新日期:2018-11-01 00:00:00
abstract::Eukaryotic cells license far more origins than are actually used for DNA replication, thereby generating a large number of dormant origins. Accumulating evidence suggests that such origins play a role in chromosome stability and tumor suppression, though the underlying mechanism is largely unknown. Here, we show that ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.02.006
更新日期:2011-03-04 00:00:00
abstract::PARP enzymes are increasingly taking on important roles beyond DNA repair. Huang et al. (2020b) report how the NAD+-dependent ADP-ribosylation of histone H2B by PARP-1 in complex with a metabolic enzyme suppresses the phosphorylation of an adjacent residue, impacting adipogenesis. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2020.08.019
更新日期:2020-09-17 00:00:00
abstract::In this issue of Molecular Cell, Lu et al. (2019) analyze the role of the length and sequence complexity of the RNA polymerase II unstructured C-terminal domain in animal viability, development, and the dynamics of RNA polymerase II in vivo. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2019.03.004
更新日期:2019-03-21 00:00:00
abstract::Deletion of elongation factor-like 1 (Efl1p), a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2, results in nucle(ol)ar pre-rRNA processing and pre-60S subunits export defects. Efl1p interacts genetically with Tif6p, a nucle(ol)ar protein stably associated with pre-60S subunits and required for t...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00403-8
更新日期:2001-12-01 00:00:00
abstract::The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00824-9
更新日期:2003-01-01 00:00:00
abstract::BIK protein is an initiator of mitochondrial apoptosis, and BIK expression is induced by proapoptotic signals, including DNA damage. Here, we demonstrate that 3' end processing and expression of BIK mRNA are controlled by the nuclear PI4,5P(2)-regulated poly(A) polymerase Star-PAP downstream of DNA damage. Nuclear PKC...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.11.017
更新日期:2012-01-13 00:00:00
abstract::One of the oldest questions in RNA science is the role of nucleotide modification. Here, the importance of pseudouridine formation (Psi) in the peptidyl transferase center of rRNA was examined by depleting yeast cells of 1-5 snoRNAs that guide a total of six Psi modifications. Translation was impaired substantially wi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00040-6
更新日期:2003-02-01 00:00:00
abstract::Cardozo Gizzi et al. (2019) develop a new sequential imaging methodology (Hi-M) for observing chromosome structure in the Drosophila blastoderm and find that topological domains in single nuclei change in response to transcriptional activation. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2019.03.017
更新日期:2019-04-04 00:00:00
abstract::BRCA1 functions at two distinct steps during homologous recombination (HR). Initially, it promotes DNA end resection, and subsequently it recruits the PALB2 and BRCA2 mediator complex, which stabilizes RAD51-DNA nucleoprotein filaments. Loss of 53BP1 rescues the HR defect in BRCA1-deficient cells by increasing resecti...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.12.010
更新日期:2019-03-21 00:00:00
abstract::The RNA-binding protein HuR, while known to stabilize cytoplasmic mRNAs, is largely nuclear. In this issue of Molecular Cell, Mukherjee et al. (2011) and Lebedeva et al. (2011) identify transcriptome-wide HuR-RNA interactions using PAR-CLIP, unveiling HuR's nuclear role in pre-mRNA processing. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2011.07.016
更新日期:2011-08-05 00:00:00
abstract::The eukaryotic RNA exosome is an essential and conserved 3'-to-5' exoribonuclease complex that degrades or processes nearly every class of cellular RNA. The nuclear RNA exosome includes a 9-subunit non-catalytic core that binds Rrp44 (Dis3) and Rrp6 subunits to modulate their processive and distributive 3'-to-5' exori...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.09.038
更新日期:2016-11-17 00:00:00
abstract::HIV-1 Nef plays important roles in HIV-1 replication and pathogenesis. It is translated from completely spliced HIV-1 RNA, and its expression is inherently regulated at the levels of viral DNA transcription and RNA splicing. Here we show that Sam68 cytoplasmic mutants potently suppress Nef expression. The suppression ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2008.11.024
更新日期:2009-01-16 00:00:00
abstract::In this issue of Molecular Cell, Conaway and colleagues (Yao et al., 2008) provide a glimpse into an interesting mechanism to control a deubiquitylating enzyme via interaction of two complexes-the 19S proteasome regulatory particle and an ATP-dependent nucleosome remodeling complex. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2008.09.005
更新日期:2008-09-26 00:00:00
abstract::The SMN complex assembles Sm cores on snRNAs, a key step in the biogenesis of snRNPs, the spliceosome's major components. Here, using SMN complex inhibitors identified by high-throughput screening and a ribo-proteomic strategy on formaldehyde crosslinked RNPs, we dissected this pathway in cells. We show that protein s...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2010.03.014
更新日期:2010-05-28 00:00:00
abstract::MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2012.10.002
更新日期:2012-12-14 00:00:00