当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > A Eukaryotic Sensor for Membrane Lipid Saturation.

A Eukaryotic Sensor for Membrane Lipid Saturation.

Abstract：

:Maintaining a fluid bilayer is essential for cell signaling and survival. Lipid saturation is a key factor determining lipid packing and membrane fluidity, and it must be tightly controlled to guarantee organelle function and identity. A dedicated eukaryotic mechanism of lipid saturation sensing, however, remains elusive. Here we show that Mga2, a transcription factor conserved among fungi, acts as a lipid-packing sensor in the ER membrane to control the production of unsaturated fatty acids. Systematic mutagenesis, molecular dynamics simulations, and electron paramagnetic resonance spectroscopy identify a pivotal role of the oligomeric transmembrane helix (TMH) of Mga2 for intra-membrane sensing, and they show that the lipid environment controls the proteolytic activation of Mga2 by stabilizing alternative rotational orientations of the TMH region. This work establishes a eukaryotic strategy of lipid saturation sensing that differs significantly from the analogous bacterial mechanism relying on hydrophobic thickness.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Covino R,Ballweg S,Stordeur C,Michaelis JB,Puth K,Wernig F,Bahrami A,Ernst AM,Hummer G,Ernst R

doi

10.1016/j.molcel.2016.05.015

subject

Has Abstract

pub_date

2016-07-07 00:00:00

pages

49-59

issue

1

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(16)30178-2

journal_volume

63

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation.

    abstract：:The formation of silenced and condensed heterochromatin foci involves enrichment of heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are elusive. Here, we assess mechanisticall...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2020.02.005

    authors： Erdel F,Rademacher A,Vlijm R,Tünnermann J,Frank L,Weinmann R,Schweigert E,Yserentant K,Hummert J,Bauer C,Schumacher S,Al Alwash A,Normand C,Herten DP,Engelhardt J,Rippe K

    更新日期：2020-04-16 00:00:00

  • A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends.

    abstract：:The mechanisms by which telomeres are distinguished from DNA double-strand breaks are poorly understood. Here we have defined the minimal requirements for the protection of telomeric DNA ends from nonhomologous end-joining (NHEJ). Neither long, single-stranded overhangs nor t loop formation is essential to prevent NHE...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.03.023

    authors： Bae NS,Baumann P

    更新日期：2007-05-11 00:00:00

  • Homologous recombination is promoted by translesion polymerase poleta.

    abstract：:Two new studies provide in vivo ( [this issue of Molecular Cell]) and in vitro ( [this issue of Molecular Cell]) evidence that poleta functions to extend 3' strands exchanged during homologous recombination and raise the issue of how TLS polymerases are selected onto different substrates. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章,评审

    doi：10.1016/j.molcel.2005.11.018

    authors： Rattray AJ,Strathern JN

    更新日期：2005-12-09 00:00:00

  • Crossover and noncrossover pathways in mouse meiosis.

    abstract：:During meiosis, recombination between homologous chromosomes generates crossover (CR) and noncrossover (NCR) products. CRs establish connections between homologs, whereas intermediates leading to NCRs have been proposed to participate in homologous pairing. How these events are differentiated and regulated remains to ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.09.021

    authors： Guillon H,Baudat F,Grey C,Liskay RM,de Massy B

    更新日期：2005-11-23 00:00:00

  • Detection of high-affinity and sliding clamp modes for MSH2-MSH6 by single-molecule unzipping force analysis.

    abstract：:Mismatch repair (MMR) is initiated by MutS family proteins (MSH) that recognize DNA mismatches and recruit downstream repair factors. We used a single-molecule DNA-unzipping assay to probe interactions between S. cerevisiae MSH2-MSH6 and a variety of DNA mismatch substrates. This work revealed a high-specificity bindi...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.10.014

    authors： Jiang J,Bai L,Surtees JA,Gemici Z,Wang MD,Alani E

    更新日期：2005-12-09 00:00:00

  • Late S phase-specific recruitment of Mre11 complex triggers hierarchical assembly of telomere replication proteins in Saccharomyces cerevisiae.

    abstract：:In Saccharomyces cerevisiae, telomere replication occurs in late S phase and is accompanied by dynamic remodeling of its protein components. Here, we show that MRX (Mre11-Rad50-Xrs2), an evolutionarily conserved protein complex involved in DNA double-strand break (DSB) repair, is recruited to the telomeres in late S p...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.01.014

    authors： Takata H,Tanaka Y,Matsuura A

    更新日期：2005-02-18 00:00:00

  • Eukaryotic stress granules: the ins and outs of translation.

    abstract：:The stress response in eukaryotic cells often inhibits translation initiation and leads to the formation of cytoplasmic RNA-protein complexes referred to as stress granules. Stress granules contain nontranslating mRNAs, translation initiation components, and many additional proteins affecting mRNA function. Stress gra...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2009.11.020

    authors： Buchan JR,Parker R

    更新日期：2009-12-25 00:00:00

  • Mitochondrial disulfide bond formation is driven by intersubunit electron transfer in Erv1 and proofread by glutathione.

    abstract：:The disulfide relay system in the intermembrane space of mitochondria is of crucial importance for mitochondrial biogenesis. Major players in this pathway are the oxidoreductase Mia40 that oxidizes substrates and the sulfhydryl oxidase Erv1 that reoxidizes Mia40. To analyze in detail the mechanism of this oxidative pa...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.01.017

    authors： Bien M,Longen S,Wagener N,Chwalla I,Herrmann JM,Riemer J

    更新日期：2010-02-26 00:00:00

  • Quantifying ubiquitin signaling.

    abstract：:Ubiquitin (UB)-driven signaling systems permeate biology, and are often integrated with other types of post-translational modifications (PTMs), including phosphorylation. Flux through such pathways is dictated by the fractional stoichiometry of distinct modifications and protein assemblies as well as the spatial organ...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2015.02.020

    authors： Ordureau A,Münch C,Harper JW

    更新日期：2015-05-21 00:00:00

  • HDAC6 modulates cell motility by altering the acetylation level of cortactin.

    abstract：:Histone deacetylase 6 (HDAC6) is a tubulin-specific deacetylase that regulates microtubule-dependent cell movement. In this study, we identify the F-actin-binding protein cortactin as a HDAC6 substrate. We demonstrate that HDAC6 binds cortactin and that overexpression of HDAC6 leads to hypoacetylation of cortactin, wh...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.05.033

    authors： Zhang X,Yuan Z,Zhang Y,Yong S,Salas-Burgos A,Koomen J,Olashaw N,Parsons JT,Yang XJ,Dent SR,Yao TP,Lane WS,Seto E

    更新日期：2007-07-20 00:00:00

  • A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.

    abstract：:The proteolytic activation of the membrane-associated transcription factor pro-sigma(K) is controlled by a signal transduction pathway during sporulation in the bacterium Bacillus subtilis. The pro-sigma(K) processing enzyme SpoIVFB, a membrane-embedded metalloprotease, is held inactive by two other integral-membrane ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.05.019

    authors： Campo N,Rudner DZ

    更新日期：2006-07-07 00:00:00

  • A global profile of germline gene expression in C. elegans.

    abstract：:We used DNA microarrays to profile gene expression patterns in the C. elegans germline and identified 1416 germline-enriched transcripts that define three groups. The sperm-enriched group contains an unusually large number of protein kinases and phosphatases. The oocyte-enriched group includes potentially new componen...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)00059-9

    authors： Reinke V,Smith HE,Nance J,Wang J,Van Doren C,Begley R,Jones SJ,Davis EB,Scherer S,Ward S,Kim SK

    更新日期：2000-09-01 00:00:00

  • Impeding DNA Break Repair Enables Oocyte Quality Control.

    abstract：:Oocyte quality control culls eggs with defects in meiosis. In mouse, oocyte death can be triggered by defects in chromosome synapsis and recombination, which involve repair of DNA double-strand breaks (DSBs) between homologous chromosomes. We show that RNF212, a SUMO ligase required for crossing over, also mediates oo...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.08.031

    authors： Qiao H,Rao HBDP,Yun Y,Sandhu S,Fong JH,Sapre M,Nguyen M,Tham A,Van BW,Chng TYH,Lee A,Hunter N

    更新日期：2018-10-18 00:00:00

  • Oligomeric initiator protein-mediated DNA looping negatively regulates plasmid replication in vitro by preventing origin melting.

    abstract：:Although DNA looping between the initiator binding sites (iterons) of the replication origin (ori) of a plasmid and the iterons located in a cis-acting control sequence called inc has been postulated to promote negative control of plasmid DNA replication, not only was definitive evidence for such looping lacking, but ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.10.037

    authors： Zzaman S,Bastia D

    更新日期：2005-12-22 00:00:00

  • Telomeres thrown for a loop.

    abstract：:A remarkable paper from the de Lange lab (Wang et al., 2004) in a recent issue of Cell reveals that homologous recombination can result in the abrupt shortening of telomeres in a process that appears to involve reciprocal crossing over within the t-loop structure that protects chromosome ends. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2004.11.006

    authors： Haber JE

    更新日期：2004-11-19 00:00:00

  • Gemin5 delivers snRNA precursors to the SMN complex for snRNP biogenesis.

    abstract：:The SMN complex assembles Sm cores on snRNAs, a key step in the biogenesis of snRNPs, the spliceosome's major components. Here, using SMN complex inhibitors identified by high-throughput screening and a ribo-proteomic strategy on formaldehyde crosslinked RNPs, we dissected this pathway in cells. We show that protein s...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.03.014

    authors： Yong J,Kasim M,Bachorik JL,Wan L,Dreyfuss G

    更新日期：2010-05-28 00:00:00

  • The structure of the bacteriophage PRD1 spike sheds light on the evolution of viral capsid architecture.

    abstract：:Comparisons of bacteriophage PRD1 and adenovirus protein structures and virion architectures have been instrumental in unraveling an evolutionary relationship and have led to a proposal of a phylogeny-based virus classification. The structure of the PRD1 spike protein P5 provides further insight into the evolution of ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.03.019

    authors： Merckel MC,Huiskonen JT,Bamford DH,Goldman A,Tuma R

    更新日期：2005-04-15 00:00:00

  • Controlling the elongation phase of transcription with P-TEFb.

    abstract：:The positive transcription elongation factor b (P-TEFb) is a cyclin-dependent kinase that controls the elongation phase of transcription by RNA polymerase II (RNAPII). This process is made possible by the reversal of effects of negative elongation factors that include NELF and DSIF. In complex organisms, elongation co...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2006.06.014

    authors： Peterlin BM,Price DH

    更新日期：2006-08-04 00:00:00

  • MicroRNA-451 Escapes Global MicroRNA Crisis by Clustered Neighboring MicroRNA-144 During Erythropoiesis.

    abstract：:In a recent issue of Molecular Cell, Kretov et al. (2020) demonstrate that microRNA-144 targets Dicer in a negative feedback loop, affecting global canonical microRNA expression in erythrocytes. MicroRNA-451 is refractory to the loss of Dicer because of its Ago2-dependent processing. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2020.04.028

    authors： Jiang S

    更新日期：2020-06-04 00:00:00

  • Molecular Basis for Hormone Recognition and Activation of Corticotropin-Releasing Factor Receptors.

    abstract：:Corticotropin-releasing factor (CRF) and the three related peptides urocortins 1-3 (UCN1-UCN3) are endocrine hormones that control the stress responses by activating CRF1R and CRF2R, two members of class B G-protein-coupled receptors (GPCRs). Here, we present two cryoelectron microscopy (cryo-EM) structures of UCN1-bo...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2020.01.013

    authors： Ma S,Shen Q,Zhao LH,Mao C,Zhou XE,Shen DD,de Waal PW,Bi P,Li C,Jiang Y,Wang MW,Sexton PM,Wootten D,Melcher K,Zhang Y,Xu HE

    更新日期：2020-02-06 00:00:00

  • Of Dots and Stripes: The Morse Code of Micro-C Reveals the Ultrastructure of Transcriptional and Architectural Mammalian 3D Genome Organization.

    abstract：:Using Micro-C, Hsieh et al. (2020) and Krietenstein et al. (2020) investigated 3D chromatin folding in human and mouse cells at unprecedented resolution to uncover ultra-fine-scale chromatin structures that provide direct links between genome architecture, gene expression, and gene regulation. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2020.04.021

    authors： Chang LH,Noordermeer D

    更新日期：2020-05-07 00:00:00

  • Nucleosomes are context-specific, H2A.Z-modulated barriers to RNA polymerase.

    abstract：:Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA polymerase II (RNAPII) at nucleotide resolution. We find that the entry site of the first ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.02.014

    authors： Weber CM,Ramachandran S,Henikoff S

    更新日期：2014-03-06 00:00:00

  • Division of Labor between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment.

    abstract：:Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including the multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2020.03.017

    authors： Liu HW,Bouchoux C,Panarotto M,Kakui Y,Patel H,Uhlmann F

    更新日期：2020-05-21 00:00:00

  • MitoCPR: Meticulous Monitoring of Mitochondrial Proteostasis.

    abstract：:Mitochondrial protein import stress compromises functioning of the organelles, due to inadequate supply of inner mitochondrial proteins. Weidberg and Amon (2018) describe a new monitoring pathway in budding yeast, which restores mitochondrial function following the clearing of accumulated unfolded pre-transported mito...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2018.06.027

    authors： Desai R,Campanella M

    更新日期：2018-07-05 00:00:00

  • Hierarchical modularity and the evolution of genetic interactomes across species.

    abstract：:To date, cross-species comparisons of genetic interactomes have been restricted to small or functionally related gene sets, limiting our ability to infer evolutionary trends. To facilitate a more comprehensive analysis, we constructed a genome-scale epistasis map (E-MAP) for the fission yeast Schizosaccharomyces pombe...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2012.05.028

    authors： Ryan CJ,Roguev A,Patrick K,Xu J,Jahari H,Tong Z,Beltrao P,Shales M,Qu H,Collins SR,Kliegman JI,Jiang L,Kuo D,Tosti E,Kim HS,Edelmann W,Keogh MC,Greene D,Tang C,Cunningham P,Shokat KM,Cagney G,Svensson JP,Gut

    更新日期：2012-06-08 00:00:00

  • RNA Polymerase Pausing during Initial Transcription.

    abstract：:In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied ini...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.08.011

    authors： Duchi D,Bauer DL,Fernandez L,Evans G,Robb N,Hwang LC,Gryte K,Tomescu A,Zawadzki P,Morichaud Z,Brodolin K,Kapanidis AN

    更新日期：2016-09-15 00:00:00

  • TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.

    abstract：:The Bloom syndrome helicase BLM and topoisomerase-IIβ-binding protein 1 (TopBP1) are key regulators of genome stability. It was recently proposed that BLM phosphorylation on Ser338 mediates its interaction with TopBP1, to protect BLM from ubiquitylation and degradation (Wang et al., 2013). Here, we show that the BLM-T...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.02.012

    authors： Blackford AN,Nieminuszczy J,Schwab RA,Galanty Y,Jackson SP,Niedzwiedz W

    更新日期：2015-03-19 00:00:00

  • Deamidation of Bcl-X(L): a new twist in a genotoxic murder mystery.

    abstract：:Bcl-2 proteins are critical regulators of apoptosis following DNA damage. Recent studies have shown that genotoxic stress induces the deamidation of Bcl-X(L), inhibiting its antiapoptotic activities. Remarkably, Rb and p53 are important regulators of this novel modification of Bcl-X(L). ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(02)00693-7

    authors： Johnstone RW

    更新日期：2002-10-01 00:00:00

  • Topoisomerase II inactivation prevents the completion of DNA replication in budding yeast.

    abstract：:Type II topoisomerases are essential for resolving topologically entwined double-stranded DNA. Although anti-topoisomerase 2 (Top2) drugs are clinically important antibiotics and chemotherapies, to our knowledge, the mechanisms of cell killing by Top2 depletion and inactivation have never been directly compared. We sh...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.04.019

    authors： Baxter J,Diffley JF

    更新日期：2008-06-20 00:00:00

  • A conserved domain within Arc1p delivers tRNA to aminoacyl-tRNA synthetases.

    abstract：:Two yeast enzymes that catalyze aminoacylation of tRNAs, MetRS and GluRS, form a complex with the protein Arc1p. We show here that association of Arc1p with MetRS and GluRS is required in vivo for effective recruitment of the corresponding cognate tRNAs within this complex. Arc1p is linked to MetRS and GluRS through i...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80024-6

    authors： Simos G,Sauer A,Fasiolo F,Hurt EC

    更新日期：1998-01-01 00:00:00