Abstract：
:Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA polymerase II (RNAPII) at nucleotide resolution. We find that the entry site of the first (+1) nucleosome is a barrier to RNAPII for essentially all genes, including those undergoing regulated pausing farther upstream. In contrast to the +1 nucleosome, gene body nucleosomes are low barriers and cause RNAPII stalling both at the entry site and near the dyad axis. The extent of the +1 nucleosome barrier correlates with nucleosome occupancy but anticorrelates with enrichment of histone variant H2A.Z. Importantly, depletion of H2A.Z from a nucleosome position results in a higher barrier to RNAPII. Our results suggest that nucleosomes present significant, context-specific barriers to RNAPII in vivo that can be tuned by the incorporation of H2A.Z.
journal_name
Mol Celljournal_title
Molecular cellauthors
Weber CM,Ramachandran S,Henikoff Sdoi
10.1016/j.molcel.2014.02.014subject
Has Abstractpub_date
2014-03-06 00:00:00pages
819-30issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(14)00159-2journal_volume
53pub_type
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