Abstract：
:Signal-transduction cascades are usually studied on cell averages, masking variability between individual cells. To address this, we studied in individual cells the dynamic response of ERK2, a well-characterized MAPK signaling protein, which enters the nucleus upon stimulation. Using fluorescent tagging at the endogenous chromosomal locus, we found that cells show wide basal variation in ERK2 nuclear levels. Upon EGF stimulation, cells show (1) a fold-change response, where peak nuclear accumulation of ERK2 is proportional to basal level in each cell; and (2) exact adaptation in nuclear levels of ERK2, returning to original basal level of each cell. The timing of ERK2 dynamics is more precise between cells than its amplitude. We further found that in some cells ERK2 exhibits a second pulse of nuclear entry, smaller than the first. The present study suggests that this signaling system compensates for natural biological noise: despite large variation in nuclear basal levels, ERK2's fold dynamics is similar between cells.
journal_name
Mol Celljournal_title
Molecular cellauthors
Cohen-Saidon C,Cohen AA,Sigal A,Liron Y,Alon Udoi
10.1016/j.molcel.2009.11.025subject
Has Abstractpub_date
2009-12-11 00:00:00pages
885-93issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(09)00866-1journal_volume
36pub_type
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