Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation.

abstract：:Deletion of elongation factor-like 1 (Efl1p), a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2, results in nucle(ol)ar pre-rRNA processing and pre-60S subunits export defects. Efl1p interacts genetically with Tif6p, a nucle(ol)ar protein stably associated with pre-60S subunits and required for t...

journal_title：Molecular cell

authors： Senger B,Lafontaine DL,Graindorge JS,Gadal O,Camasses A,Sanni A,Garnier JM,Breitenbach M,Hurt E,Fasiolo F

更新日期：2001-12-01 00:00:00

abstract：:In this issue of Molecular Cell, Lim et al. (2010) show that SIRT1 deacetylates HIF-1alpha and regulates its ability to respond to hypoxia, revealing yet another important function of SIRT1 and suggesting a connection between HIF function in aging and sirtuin enzymes. ...

journal_title：Molecular cell

authors： Leiser SF,Kaeberlein M

更新日期：2010-06-25 00:00:00

abstract：:One of the oldest questions in RNA science is the role of nucleotide modification. Here, the importance of pseudouridine formation (Psi) in the peptidyl transferase center of rRNA was examined by depleting yeast cells of 1-5 snoRNAs that guide a total of six Psi modifications. Translation was impaired substantially wi...

journal_title：Molecular cell

authors： King TH,Liu B,McCully RR,Fournier MJ

更新日期：2003-02-01 00:00:00

abstract：:In this issue of Molecular Cell, Guo et al. (2014) report that misfolded or aggregated nuclear proteins, such as pathogenic polyQ proteins, are cleared by a SUMO-dependent quality control pathway, which involves the E3 SUMO ligase PML and the SUMO-targeted ubiquitin ligase RNF4. ...

journal_title：Molecular cell

authors： Gärtner A,Muller S

更新日期：2014-07-03 00:00:00

abstract：:Neurons undertake an amazing journey from the center of the developing mammalian brain to the outer layers of the cerebral cortex. Doublecortin, a component of the microtubule cytoskeleton, is essential in postmitotic neurons and was identified because its mutation disrupts human brain development. Doublecortin stabil...

journal_title：Molecular cell

authors： Moores CA,Perderiset M,Francis F,Chelly J,Houdusse A,Milligan RA

更新日期：2004-06-18 00:00:00

abstract：:Inhibition of apoptotic response of host cells during an early phase of infection is a strategy used by many enteroinvasive bacterial pathogens to enhance their survival. Here, we report the identification of a soluble form of the pilus protein FimA from the culture supernatants of E. coli K1, Salmonella, and Shigella...

journal_title：Molecular cell

authors： Sukumaran SK,Fu NY,Tin CB,Wan KF,Lee SS,Yu VC

更新日期：2010-03-26 00:00:00

abstract：:Among the five KCNQ channels, also known as the Kv7 voltage-gated potassium (Kv) channels, KCNQ2-KCNQ5 control neuronal excitability. Dysfunctions of KCNQ2-KCNQ5 are associated with neurological disorders such as epilepsy, deafness, and neuropathic pain. Here, we report the cryoelectron microscopy (cryo-EM) structures...

journal_title：Molecular cell

authors： Li T,Wu K,Yue Z,Wang Y,Zhang F,Shen H

更新日期：2021-01-07 00:00:00

abstract：:CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...

journal_title：Molecular cell

authors： Wright AV,Wang JY,Burstein D,Harrington LB,Paez-Espino D,Kyrpides NC,Iavarone AT,Banfield JF,Doudna JA

更新日期：2019-02-21 00:00:00

abstract：:Mammalian cells constantly monitor and respond to a myriad of extracellular signals, often by using cell surface receptors. Two important classes of cell surface receptors include the receptor tyrosine kinases, which recognize peptide growth factors such as insulin, and the integrins, which most often mediate binding ...

journal_title：Molecular cell

authors： Shrivastava A,Radziejewski C,Campbell E,Kovac L,McGlynn M,Ryan TE,Davis S,Goldfarb MP,Glass DJ,Lemke G,Yancopoulos GD

更新日期：1997-12-01 00:00:00

abstract：:The essential functions of the conserved Smc5/6 complex remain elusive. To uncover its roles in genome maintenance, we established Saccharomyces cerevisiae cell-cycle-regulated alleles that enable restriction of Smc5/6 components to S or G2/M. Unexpectedly, the essential functions of Smc5/6 segregated fully and select...

journal_title：Molecular cell

authors： Menolfi D,Delamarre A,Lengronne A,Pasero P,Branzei D

更新日期：2015-12-17 00:00:00

abstract：:Polycomb proteins are well-known epigenetic repressors with unexplained roles in chromatin folding. In this issue of Molecular Cell, Kundu et al. (2017) investigate the structures of PRC1-mediated domains in stem cells and probe their changes upon differentiation and in PRC knockouts. ...

journal_title：Molecular cell

authors： Torlai Triglia E,Rito T,Pombo A

更新日期：2017-02-02 00:00:00

abstract：:In this issue of Molecular Cell, Schmidt et al. (2009) untangle the interplay between the COP9 signalosome (CSN), cullin-associated and neddylation-dissociated 1 (CAND1) protein, and cullin-RING ubiquitin ligases (CRLs). ...

journal_title：Molecular cell

authors： Dubiel W

更新日期：2009-09-11 00:00:00

abstract：:The role of the protein kinase Akt in cell migration is incompletely understood. Here we show that sphingosine-1-phosphate (S1P)-induced endothelial cell migration requires the Akt-mediated phosphorylation of the G protein-coupled receptor (GPCR) EDG-1. Activated Akt binds to EDG-1 and phosphorylates the third intrace...

journal_title：Molecular cell

authors： Lee MJ,Thangada S,Paik JH,Sapkota GP,Ancellin N,Chae SS,Wu M,Morales-Ruiz M,Sessa WC,Alessi DR,Hla T

更新日期：2001-09-01 00:00:00

abstract：:Metazoan histone mRNAs are unique: their pre-mRNAs contain no introns, and the mRNAs are not polyadenylated, ending instead in a conserved stem-loop structure. In Drosophila, canonical poly(A) signals are located downstream of the normal cleavage site of each histone gene and are utilized when histone 3' end formation...

journal_title：Molecular cell

authors： Sullivan KD,Steiniger M,Marzluff WF

更新日期：2009-05-15 00:00:00

abstract：:Bcl-2 proteins are critical regulators of apoptosis following DNA damage. Recent studies have shown that genotoxic stress induces the deamidation of Bcl-X(L), inhibiting its antiapoptotic activities. Remarkably, Rb and p53 are important regulators of this novel modification of Bcl-X(L). ...

journal_title：Molecular cell

authors： Johnstone RW

更新日期：2002-10-01 00:00:00

abstract：:AAA ATPases play central roles in cellular activities. The ATPase p97, a prototype of this superfamily, participates in organelle membrane fusion. Cryoelectron microscopy and single-particle analysis revealed that a major conformational change of p97 during the ATPase cycle occurred upon nucleotide binding and not dur...

journal_title：Molecular cell

authors： Rouiller I,Butel VM,Latterich M,Milligan RA,Wilson-Kubalek EM

更新日期：2000-12-01 00:00:00

abstract：:Posttranslational modification with small ubiquitin-related modifier (SUMO) has emerged as a central regulatory mechanism of protein function. However, little is known about the regulation of sumoylation itself. It has been reported that it is increased after exposure to various stresses including strong oxidative str...

journal_title：Molecular cell

authors： Bossis G,Melchior F

更新日期：2006-02-03 00:00:00

abstract：:In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq...

journal_title：Molecular cell

authors： Tree JJ,Granneman S,McAteer SP,Tollervey D,Gally DL

更新日期：2014-07-17 00:00:00

abstract：:Mitochondrial protein import stress compromises functioning of the organelles, due to inadequate supply of inner mitochondrial proteins. Weidberg and Amon (2018) describe a new monitoring pathway in budding yeast, which restores mitochondrial function following the clearing of accumulated unfolded pre-transported mito...

journal_title：Molecular cell

authors： Desai R,Campanella M

更新日期：2018-07-05 00:00:00

abstract：:Tumor suppressor genes evolved as negative effectors of mitogen and nutrient signaling pathways, such that mutations in these genes can lead to pathological states of growth. Tuberous sclerosis (TSC) is a potentially devastating disease associated with mutations in two tumor suppressor genes, TSC1 and 2, that function...

journal_title：Molecular cell

authors： Garami A,Zwartkruis FJ,Nobukuni T,Joaquin M,Roccio M,Stocker H,Kozma SC,Hafen E,Bos JL,Thomas G

更新日期：2003-06-01 00:00:00

abstract：:E1 enzymes activate ubiquitin or related proteins and pass them to E2 enzymes. A recent structure and associated biochemical studies (Huang et al., 2005) show how an E1 binds its cognate E2 and indicates that large, conformational changes will be an integral component of the E1 reaction cycle. ...

journal_title：Molecular cell

authors： VanDemark AP,Hill CP

更新日期：2005-02-18 00:00:00

abstract：:Immune cell function depends on specific metabolic programs dictated by mitochondria, including nutrient oxidation, macromolecule synthesis, and post-translational modifications. Mitochondrial adaptations have been linked to acute and chronic inflammation, but the metabolic cues and precise mechanisms remain unclear. ...

journal_title：Molecular cell

authors： Chi Z,Chen S,Xu T,Zhen W,Yu W,Jiang D,Guo X,Wang Z,Zhang K,Li M,Zhang J,Fang H,Yang D,Ye Q,Yang X,Lin H,Yang F,Zhang X,Wang D

更新日期：2020-10-01 00:00:00

abstract：:A paradigm in transcriptional regulation is that graded increases in transcription factor (TF) concentration are translated into on/off transcriptional responses by cooperative TF binding to adjacent sites. Digital transcriptional responses underlie the definition of anatomical boundaries during development. Here we s...

journal_title：Molecular cell

authors： Giorgetti L,Siggers T,Tiana G,Caprara G,Notarbartolo S,Corona T,Pasparakis M,Milani P,Bulyk ML,Natoli G

更新日期：2010-02-12 00:00:00

abstract：:How repeating striped patterns arise across cellular fields is unclear. To address this we examined the repeating pattern of Stripe (Sr) expression across the parasegment (PS) in Drosophila. This pattern is generated in two steps. First, the ligands Hedgehog (Hh) and Wingless (Wg) subdivide the PS into smaller territo...

journal_title：Molecular cell

authors： Hatini V,DiNardo S

更新日期：2001-01-01 00:00:00

abstract：:In this issue of Molecular Cell, detail molecular events that trigger apoptosis following growth factor withdrawal, linking irreversible mitochondrial permeabilization to activation of GSK-3 and subsequent phosphorylation, ubiquitinylation, and degradation of the antiapoptotic BCL-2 family member MCL-1. ...

journal_title：Molecular cell

authors： Letai A

更新日期：2006-03-17 00:00:00

abstract：:Hydroxyl radical footprinting and directed probing from Fe(II)-derivatized IF3 have been used to map the interaction of IF3 relative to 16S rRNA and tRNA(Met)(f) in the 30S ribosomal subunit. Our results place the two domains of IF3 on opposite sides of the initiator tRNA, with the C domain at the platform interface a...

journal_title：Molecular cell

authors： Dallas A,Noller HF

更新日期：2001-10-01 00:00:00

abstract：:Genome instability, a hallmark of cancer progression, is thought to arise through DNA double strand breaks (DSBs). Studies in yeast and mammalian cells have shown that DSBs and instability can occur through RNA:DNA hybrids generated by defects in RNA elongation and splicing. We report that in yeast hybrids naturally f...

journal_title：Molecular cell

authors： Wahba L,Amon JD,Koshland D,Vuica-Ross M

更新日期：2011-12-23 00:00:00

abstract：:Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard test. A GATA-2-binding, Gata2 intronic cis-element (+9.5) required ...

journal_title：Molecular cell

authors： Hewitt KJ,Kim DH,Devadas P,Prathibha R,Zuo C,Sanalkumar R,Johnson KD,Kang YA,Kim JS,Dewey CN,Keles S,Bresnick EH

更新日期：2015-07-02 00:00:00

abstract：:The methylcytosine oxidase TET proteins play important roles in DNA demethylation and development. However, it remains elusive how exactly they target substrates and execute oxidation. Interestingly, we found that, in mice, the full-length TET1 isoform (TET1e) is restricted to early embryos, embryonic stem cells (ESCs...

journal_title：Molecular cell

authors： Zhang W,Xia W,Wang Q,Towers AJ,Chen J,Gao R,Zhang Y,Yen CA,Lee AY,Li Y,Zhou C,Liu K,Zhang J,Gu TP,Chen X,Chang Z,Leung D,Gao S,Jiang YH,Xie W

更新日期：2016-12-15 00:00:00

abstract：:Innate immune responses are critical for the immediate protection against microbial infection. In Drosophila, infection leads to the rapid and robust production of antimicrobial peptides through two NF-kappaB signaling pathways-IMD and Toll. The IMD pathway is triggered by DAP-type peptidoglycan, common to most Gram-n...

journal_title：Molecular cell

authors： Paquette N,Broemer M,Aggarwal K,Chen L,Husson M,Ertürk-Hasdemir D,Reichhart JM,Meier P,Silverman N

更新日期：2010-01-29 00:00:00