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A Multiplexed System for Quantitative Comparisons of Chromatin Landscapes.

Abstract：

:Genome-wide profiling of histone modifications can provide systematic insight into the regulatory elements and programs engaged in a given cell type. However, conventional chromatin immunoprecipitation and sequencing (ChIP-seq) does not capture quantitative information on histone modification levels, requires large amounts of starting material, and involves tedious processing of each individual sample. Here, we address these limitations with a technology that leverages DNA barcoding to profile chromatin quantitatively and in multiplexed format. We concurrently map relative levels of multiple histone modifications across multiple samples, each comprising as few as a thousand cells. We demonstrate the technology by monitoring dynamic changes following inhibition of p300, EZH2, or KDM5, by linking altered epigenetic landscapes to chromatin regulator mutations, and by mapping active and repressive marks in purified human hematopoietic stem cells. Hence, this technology enables quantitative studies of chromatin state dynamics across rare cell types, genotypes, environmental conditions, and drug treatments.

journal_name

Mol Cell

journal_title

Molecular cell

authors

van Galen P,Viny AD,Ram O,Ryan RJ,Cotton MJ,Donohue L,Sievers C,Drier Y,Liau BB,Gillespie SM,Carroll KM,Cross MB,Levine RL,Bernstein BE

doi

10.1016/j.molcel.2015.11.003

subject

Has Abstract

pub_date

2016-01-07 00:00:00

pages

170-80

issue

1

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(15)00863-1

journal_volume

61

pub_type

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