Abstract：
:Aberrant folding and fibrillar aggregation by polyglutamine (polyQ) expansion proteins are associated with cytotoxicity in Huntington's disease and other neurodegenerative disorders. Hsp70 chaperones have an inhibitory effect on fibril formation and can alleviate polyQ cytotoxicity. Here we show that the cytosolic chaperonin, TRiC, functions synergistically with Hsp70 in this process and is limiting in suppressing polyQ toxicity in a yeast model. In vitro reconstitution experiments revealed that TRiC, in cooperation with the Hsp70 system, promotes the assembly of polyQ-expanded fragments of huntingtin (Htt) into soluble oligomers of approximately 500 kDa. Similar oligomers were observed in yeast cells upon TRiC overexpression and were found to be benign, in contrast to conformationally distinct Htt oligomers of approximately 200 kDa, which accumulated at normal TRiC levels and correlated with inhibition of cell growth. We suggest that TRiC cooperates with the Hsp70 system as a key component in the cellular defense against amyloid-like protein misfolding.
journal_name
Mol Celljournal_title
Molecular cellauthors
Behrends C,Langer CA,Boteva R,Böttcher UM,Stemp MJ,Schaffar G,Rao BV,Giese A,Kretzschmar H,Siegers K,Hartl FUdoi
10.1016/j.molcel.2006.08.017subject
Has Abstractpub_date
2006-09-15 00:00:00pages
887-97issue
6eissn
1097-2765issn
1097-4164pii
S1097-2765(06)00598-3journal_volume
23pub_type
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