RNA binding of PRC2: promiscuous or well ordered?

abstract：:The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, an...

journal_title：Molecular cell

authors： Zhong H,Voll RE,Ghosh S

更新日期：1998-04-01 00:00:00

abstract：:In a recent publication in Science, Kujirai et al. (2018) use single-particle cryo-EM to resolve several Pol II-nucleosome interactions, shedding new light on transcription elongation in a native chromatin environment. ...

journal_title：Molecular cell

authors： Noe Gonzalez M,Conaway RC,Conaway JW

更新日期：2018-12-06 00:00:00

abstract：:In this issue of Molecular Cell, Lee et al. (2009) report that SUMOylation of nuclear receptors LXRalpha and LXRbeta plays a critical role in the transrepression of IFN-gamma-induced STAT1-dependent inflammatory responses in brain astrocytes. ...

journal_title：Molecular cell

authors： Liu B,Shuai K

更新日期：2009-09-24 00:00:00

abstract：:Transcription of telomeric DNA has long been thought to inhibit telomerase. Using live cell imaging, in this issue, Cusanelli et al. (2013) now reveal a different scenario: telomeric RNA is preferentially generated at short telomeres and delivers telomerase to the chromosome end from which the transcript originated. ...

journal_title：Molecular cell

authors： Smekalova E,Baumann P

更新日期：2013-09-26 00:00:00

abstract：:MYOD-directed fibroblast trans-differentiation into skeletal muscle provides a unique model to investigate how one transcription factor (TF) reconfigures the three-dimensional chromatin architecture to control gene expression, which is otherwise achieved by the combinatorial activities of multiple TFs. Integrative ana...

journal_title：Molecular cell

authors： Dall'Agnese A,Caputo L,Nicoletti C,di Iulio J,Schmitt A,Gatto S,Diao Y,Ye Z,Forcato M,Perera R,Bicciato S,Telenti A,Ren B,Puri PL

更新日期：2019-11-07 00:00:00

abstract：:MRE11 within the MRE11-RAD50-NBS1 (MRN) complex acts in DNA double-strand break repair (DSBR), detection, and signaling; yet, how its endo- and exonuclease activities regulate DSBR by nonhomologous end-joining (NHEJ) versus homologous recombination (HR) remains enigmatic. Here, we employed structure-based design with ...

journal_title：Molecular cell

authors： Shibata A,Moiani D,Arvai AS,Perry J,Harding SM,Genois MM,Maity R,van Rossum-Fikkert S,Kertokalio A,Romoli F,Ismail A,Ismalaj E,Petricci E,Neale MJ,Bristow RG,Masson JY,Wyman C,Jeggo PA,Tainer JA

更新日期：2014-01-09 00:00:00

abstract：:In this issue of Molecular Cell, Lu et al. (2019) analyze the role of the length and sequence complexity of the RNA polymerase II unstructured C-terminal domain in animal viability, development, and the dynamics of RNA polymerase II in vivo. ...

journal_title：Molecular cell

authors： Isaac RS,Churchman LS

更新日期：2019-03-21 00:00:00

abstract：:The anaphase-promoting complex (APC) is a ubiquitin ligase that promotes the degradation of cell-cycle regulators. Cdh1p is an APC coactivator that directly binds APC substrates. A genetic screen in budding yeast identified residues within Cdh1p critical for its function. Cdh1p proteins containing mutations within the...

journal_title：Molecular cell

authors： Burton JL,Tsakraklides V,Solomon MJ

更新日期：2005-05-27 00:00:00

abstract：:p73 has been identified as a structural and functional homolog of the tumor suppressor p53. The transcriptional coactivator Yes-associated protein (YAP) has been demonstrated to interact with and to enhance p73-dependent apoptosis in response to DNA damage. Here, we show the existence of a proapoptotic autoregulatory ...

journal_title：Molecular cell

authors： Lapi E,Di Agostino S,Donzelli S,Gal H,Domany E,Rechavi G,Pandolfi PP,Givol D,Strano S,Lu X,Blandino G

更新日期：2008-12-26 00:00:00

abstract：:The phosphoinositide (PI)-3-kinase-related kinase (PIKK) family proteins Tel1p and Mec1p have been implicated in the telomere integrity of Saccharomyces cerevisiae. However, the mechanism of PIKK-mediated telomere length control remains unclear. Here, we show that Tel1p and Mec1p are recruited to the telomeres at spec...

journal_title：Molecular cell

authors： Takata H,Kanoh Y,Gunge N,Shirahige K,Matsuura A

更新日期：2004-05-21 00:00:00

abstract：:Kong et al. (2020) present the low-resolution structure of the ATPɣS-bound human condensin I and II complexes and demonstrate that human condensins can extrude DNA loops in a symmetric and asymmetric fashion and compact nucleosome-bound DNA. ...

journal_title：Molecular cell

authors： Goloborodko A

更新日期：2020-07-02 00:00:00

abstract：:In E. coli homologous recombination, a filament of RecA protein formed on DNA searches and pairs a homologous sequence within a second DNA molecule with remarkable speed and fidelity. Here, we directly probe the strength of the two-molecule interactions involved in homology search and recognition using dual-molecule m...

journal_title：Molecular cell

authors： De Vlaminck I,van Loenhout MT,Zweifel L,den Blanken J,Hooning K,Hage S,Kerssemakers J,Dekker C

更新日期：2012-06-08 00:00:00

abstract：:Eukaryotic neurotransmitter:sodium symporters (NSSs), targets for antidepressants and psychostimulants, terminate neurotransmission by sodium-driven reuptake. The crystal structure of LeuT(Aa), a prokaryotic NSS homolog, revealed an occluded state in which one leucine and two Na(+) ions are bound, but provided limited...

journal_title：Molecular cell

authors： Shi L,Quick M,Zhao Y,Weinstein H,Javitch JA

更新日期：2008-06-20 00:00:00

abstract：:Apoptosis, a phenomenon that allows the regulated destruction and disposal of damaged or unwanted cells, is common to many cellular processes in multicellular organisms. In humans more than 200 proteins are involved in apoptosis, many of which are dysregulated or defective in human diseases including cancer. A large n...

journal_title：Molecular cell

authors： Schwerk C,Schulze-Osthoff K

更新日期：2005-07-01 00:00:00

abstract：:Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-depe...

journal_title：Molecular cell

authors： Brown KM,Gilmartin GM

更新日期：2003-12-01 00:00:00

abstract：:When inappropriate DNA structures arise, they are sensed by DNA structure-dependent checkpoint pathways and subsequently repaired. Recruitment of checkpoint proteins to such structures precedes recruitment of proteins involved in DNA metabolism. Thus, checkpoints can regulate DNA metabolism. We show that fission yeast...

journal_title：Molecular cell

authors： Furuya K,Miyabe I,Tsutsui Y,Paderi F,Kakusho N,Masai H,Niki H,Carr AM

更新日期：2010-11-24 00:00:00

abstract：:In this issue, Takahara and Maeda (2012) discover that together, Pbp1 and sequestration of the TORC1 complex in cytoplasmic mRNP stress granules provides a negative regulatory mechanism for TORC1 signaling during stress. ...

journal_title：Molecular cell

authors： Buchan JR,Capaldi AP,Parker R

更新日期：2012-07-27 00:00:00

abstract：:HIV-1 Vpu interacts with CD4 in the endoplasmic reticulum and triggers CD4 degradation, presumably by proteasomes. Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary complexes have been detected by coimmunoprecipitation. beta TrCP binding to Vpu...

journal_title：Molecular cell

authors： Margottin F,Bour SP,Durand H,Selig L,Benichou S,Richard V,Thomas D,Strebel K,Benarous R

更新日期：1998-03-01 00:00:00

abstract：:The essential histone H3 lysine 79 methyltransferase Dot1L regulates transcription and genomic stability and is deregulated in leukemia. The activity of Dot1L is stimulated by mono-ubiquitination of histone H2B on lysine 120 (H2BK120Ub); however, the detailed mechanism is not understood. We report cryo-EM structures o...

journal_title：Molecular cell

authors： Valencia-Sánchez MI,De Ioannes P,Wang M,Vasilyev N,Chen R,Nudler E,Armache JP,Armache KJ

更新日期：2019-06-06 00:00:00

abstract：:Spt6 is an essential histone chaperone that mediates nucleosome reassembly during gene transcription. Spt6 also associates with RNA polymerase II (RNAPII) via a tandem Src2 homology domain. However, the significance of Spt6-RNAPII interaction is not well understood. Here, we show that Spt6 recruitment to genes and the...

journal_title：Molecular cell

authors： Dronamraju R,Hepperla AJ,Shibata Y,Adams AT,Magnuson T,Davis IJ,Strahl BD

更新日期：2018-06-21 00:00:00

abstract：:TMEM39A, encoding an ER-localized transmembrane protein, is a susceptibility locus for multiple autoimmune diseases. The molecular function of TMEM39A remains completely unknown. Here we demonstrated that TMEM39A, also called SUSR2, modulates autophagy activity by regulating the spatial distribution and levels of PtdI...

journal_title：Molecular cell

authors： Miao G,Zhang Y,Chen D,Zhang H

更新日期：2020-02-06 00:00:00

abstract：:We used computational algorithms to find conserved sequences in the 3' untranslated region (UTR) of transcripts that exhibited rapid decay in primary human T cells and found that the consensus sequence UGUUUGUUUGU, which we have termed a GU-rich element (GRE), was enriched in short-lived transcripts. Using a tet-off r...

journal_title：Molecular cell

authors： Vlasova IA,Tahoe NM,Fan D,Larsson O,Rattenbacher B,Sternjohn JR,Vasdewani J,Karypis G,Reilly CS,Bitterman PB,Bohjanen PR

更新日期：2008-02-01 00:00:00

abstract：:Polycomb repressive complex 2 (PRC2) places H3K27me3 at developmental genes and is causally implicated in keeping bivalent genes silent. It is unclear if that silence requires minimum H3K27me3 levels and how the mark transmits faithfully across mammalian somatic cell generations. Mouse intestinal cells lacking EZH2 me...

journal_title：Molecular cell

authors： Jadhav U,Manieri E,Nalapareddy K,Madha S,Chakrabarti S,Wucherpfennig K,Barefoot M,Shivdasani RA

更新日期：2020-04-02 00:00:00

abstract：:The presence of microbial or self DNA in the cytoplasm of mammalian cells is a danger signal detected by the DNA sensor cyclic-GMP-AMP (cGAMP) synthase (cGAS), which catalyzes the production of cGAMP that in turn serves as a second messenger to activate innate immune responses. Here we show that endogenous cGAMP in ma...

journal_title：Molecular cell

authors： Zhang X,Shi H,Wu J,Zhang X,Sun L,Chen C,Chen ZJ

更新日期：2013-07-25 00:00:00

abstract：:Structures of intact receptors with single-pass transmembrane domains are essential to understand how extracellular and cytoplasmic domains regulate association and signaling through transmembrane domains. A chemical and computational method to determine structures of the membrane regions of such receptors on the cell...

journal_title：Molecular cell

authors： Zhu J,Luo BH,Barth P,Schonbrun J,Baker D,Springer TA

更新日期：2009-04-24 00:00:00

abstract：:Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient recycling, and various aspects of growth and development in eukaryotes. Here we show that autophagy impacts the other major degradative route involving the ubiquitin-proteasome system by eliminating 26S proteasomes, a pro...

journal_title：Molecular cell

authors： Marshall RS,Li F,Gemperline DC,Book AJ,Vierstra RD

更新日期：2015-06-18 00:00:00

abstract：:The Rictor/mTOR complex (also known as mTORC2) plays a critical role in cellular homeostasis by phosphorylating AGC kinases such as Akt and SGK at their hydrophobic motifs to activate downstream signaling. However, the regulation of mTORC2 and whether it has additional function(s) remain largely unknown. Here, we repo...

journal_title：Molecular cell

authors： Gao D,Wan L,Inuzuka H,Berg AH,Tseng A,Zhai B,Shaik S,Bennett E,Tron AE,Gasser JA,Lau A,Gygi SP,Harper JW,DeCaprio JA,Toker A,Wei W

更新日期：2010-09-10 00:00:00

abstract：:Trans-sialidases (TS) are GPI-anchored surface enzymes expressed in specific developmental stages of trypanosome parasites like Trypanosoma cruzi, the etiologic agent of Chagas disease, and T. brucei, the causative agent of sleeping sickness. TS catalyzes the transfer of sialic acid residues from host to parasite glyc...

journal_title：Molecular cell

authors： Buschiazzo A,Amaya MF,Cremona ML,Frasch AC,Alzari PM

更新日期：2002-10-01 00:00:00

abstract：:Metazoan histone mRNAs end in a highly conserved stem-loop structure followed by ACCCA. Previous studies have suggested that the stem-loop binding protein (SLBP) is the only protein binding this region. Using RNA affinity purification, we identified a second protein, designated 3'hExo, that contains a SAP and a 3' exo...

journal_title：Molecular cell

authors： Dominski Z,Yang XC,Kaygun H,Dadlez M,Marzluff WF

更新日期：2003-08-01 00:00:00

abstract：:ER-associated degradation (ERAD) of glycoproteins depends on dual recognition of protein misfolding and remodeling of the substrate's N-linked glycans. After recognition, substrates are retrotranslocated to the cytosol for proteasomal degradation. To explore the directionality of this process, we fused a highly stable...

journal_title：Molecular cell

authors： Bhamidipati A,Denic V,Quan EM,Weissman JS

更新日期：2005-09-16 00:00:00