Metabolism of Free Guanidine in Bacteria Is Regulated by a Widespread Riboswitch Class.

Abstract:

:The guanidyl moiety is a component of fundamental metabolites, including the amino acid arginine, the energy carrier creatine, and the nucleobase guanine. Curiously, reports regarding the importance of free guanidine in biology are sparse, and no biological receptors that specifically recognize this compound have been previously identified. We report that many members of the ykkC motif RNA, the longest unresolved riboswitch candidate, naturally sense and respond to guanidine. This RNA is found throughout much of the bacterial domain of life, where it commonly controls the expression of proteins annotated as urea carboxylases and multidrug efflux pumps. Our analyses reveal that these proteins likely function as guanidine carboxylases and guanidine transporters, respectively. Furthermore, we demonstrate that bacteria are capable of endogenously producing guanidine. These and related findings demonstrate that free guanidine is a biologically relevant compound, and several gene families that can alleviate guanidine toxicity exist.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Nelson JW,Atilho RM,Sherlock ME,Stockbridge RB,Breaker RR

doi

10.1016/j.molcel.2016.11.019

subject

Has Abstract

pub_date

2017-01-19 00:00:00

pages

220-230

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(16)30727-4

journal_volume

65

pub_type

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