Abstract:
:The guanidyl moiety is a component of fundamental metabolites, including the amino acid arginine, the energy carrier creatine, and the nucleobase guanine. Curiously, reports regarding the importance of free guanidine in biology are sparse, and no biological receptors that specifically recognize this compound have been previously identified. We report that many members of the ykkC motif RNA, the longest unresolved riboswitch candidate, naturally sense and respond to guanidine. This RNA is found throughout much of the bacterial domain of life, where it commonly controls the expression of proteins annotated as urea carboxylases and multidrug efflux pumps. Our analyses reveal that these proteins likely function as guanidine carboxylases and guanidine transporters, respectively. Furthermore, we demonstrate that bacteria are capable of endogenously producing guanidine. These and related findings demonstrate that free guanidine is a biologically relevant compound, and several gene families that can alleviate guanidine toxicity exist.
journal_name
Mol Celljournal_title
Molecular cellauthors
Nelson JW,Atilho RM,Sherlock ME,Stockbridge RB,Breaker RRdoi
10.1016/j.molcel.2016.11.019subject
Has Abstractpub_date
2017-01-19 00:00:00pages
220-230issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(16)30727-4journal_volume
65pub_type
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