Crystal structure of a heterodimeric complex of RAR and RXR ligand-binding domains.

abstract：:In this issue of Molecular Cell, Huang et al. (2009) describe two heterotrimeric single-stranded DNA binding complexes, SOSS1 and SOSS2, that function downstream of the MRN complex to promote DNA repair and the G2/M checkpoint. ...

journal_title：Molecular cell

authors： Nam EA,Cortez D

更新日期：2009-08-14 00:00:00

abstract：:The stress response in eukaryotic cells often inhibits translation initiation and leads to the formation of cytoplasmic RNA-protein complexes referred to as stress granules. Stress granules contain nontranslating mRNAs, translation initiation components, and many additional proteins affecting mRNA function. Stress gra...

journal_title：Molecular cell

authors： Buchan JR,Parker R

更新日期：2009-12-25 00:00:00

abstract：:During transcription initiation by bacterial RNA polymerase, the sigma subunit recognizes the -35 and -10 promoter elements; free sigma, however, does not bind DNA. We selected ssDNA aptamers that strongly and specifically bound free sigma(A) from Thermus aquaticus. A consensus sequence, GTA(C/T)AATGGGA, was required ...

journal_title：Molecular cell

authors： Feklistov A,Barinova N,Sevostyanova A,Heyduk E,Bass I,Vvedenskaya I,Kuznedelov K,Merkiene E,Stavrovskaya E,Klimasauskas S,Nikiforov V,Heyduk T,Severinov K,Kulbachinskiy A

更新日期：2006-07-07 00:00:00

abstract：:Tet(O) belongs to a class of ribosomal protection proteins that mediate tetracycline resistance. It is a G protein that shows significant sequence similarity to elongation factor EF-G. Here we present a cryo-electron microscopic reconstruction, at 16 A resolution, of its complex with the E. coli 70S ribosome. Tet(O) w...

journal_title：Molecular cell

authors： Spahn CM,Blaha G,Agrawal RK,Penczek P,Grassucci RA,Trieber CA,Connell SR,Taylor DE,Nierhaus KH,Frank J

更新日期：2001-05-01 00:00:00

abstract：:Tethering of synaptic vesicles (SVs) to the active zone determines synaptic strength, although the molecular basis governing SV tethering is elusive. Here, we discover that small unilamellar vesicles (SUVs) and SVs from rat brains coat on the surface of condensed liquid droplets formed by active zone proteins RIM, RIM...

journal_title：Molecular cell

authors： Wu X,Ganzella M,Zhou J,Zhu S,Jahn R,Zhang M

更新日期：2021-01-07 00:00:00

abstract：:The mechanism by which (CTG)n expansion in the 3' UTR of the DMPK gene causes myotonic dystrophy (DM) is unknown. We identified four RNA splicing factors--hnRNP C, U2AF (U2 auxiliary factor), PTB (polypyrimidine tract binding protein), and PSF (PTB associated splicing factor)--that bind to two short regions 3' of the ...

journal_title：Molecular cell

authors： Tiscornia G,Mahadevan MS

更新日期：2000-06-01 00:00:00

abstract：:Centromeres play several important roles in ensuring proper chromosome segregation. Not only do they promote kinetochore assembly for microtubule attachment, but they also support robust sister chromatid cohesion at pericentromeres and facilitate replication of centromeric DNA early in S phase. However, it is still el...

journal_title：Molecular cell

authors： Natsume T,Müller CA,Katou Y,Retkute R,Gierliński M,Araki H,Blow JJ,Shirahige K,Nieduszynski CA,Tanaka TU

更新日期：2013-06-06 00:00:00

abstract：:There are many regulated steps in the assembly of a transcription preinitiation complex (PIC). In this issue of Molecular Cell, Black et. al. (2006) reveal a catalytic switch mechanism in which autoacetylation of the HAT p300 triggers its dissociation from a promoter in a manner that is coupled to TFIID association. ...

journal_title：Molecular cell

authors： Pugh BF

更新日期：2006-09-15 00:00:00

abstract：:In this issue, Isom et al. (2013) report their exciting discovery that G proteins can sense pH changes to fine-tune signaling in response to metabolic changes. ...

journal_title：Molecular cell

authors： Sprang SR

更新日期：2013-08-22 00:00:00

abstract：:Faithful propagation of specific chromatin states requires re-establishment of epigenetic marks after every cell division. How the original epigenetic signature is inherited after disruption during DNA replication is still poorly understood. Here, we show that the poly(ADP-ribose)-polymerase-1 (PARP1/ARTD1) is implica...

journal_title：Molecular cell

authors： Guetg C,Scheifele F,Rosenthal F,Hottiger MO,Santoro R

更新日期：2012-03-30 00:00:00

abstract：:How eukaryotic chromosomes fold inside the nucleus is an age-old question that remains unanswered today. Early biochemical and microscopic studies revealed the existence of chromatin domains and loops as a pervasive feature of interphase chromosomes, but the biological implications of such organizational features were...

journal_title：Molecular cell

authors： Dixon JR,Gorkin DU,Ren B

更新日期：2016-06-02 00:00:00

abstract：:DNA double-strand breaks (DSBs) arise during physiological transcription, DNA replication, and antigen receptor diversification. Mistargeting or misprocessing of DSBs can result in pathological structural variation and mutation. Here we describe a sensitive method (END-seq) to monitor DNA end resection and DSBs genome...

journal_title：Molecular cell

authors： Canela A,Sridharan S,Sciascia N,Tubbs A,Meltzer P,Sleckman BP,Nussenzweig A

更新日期：2016-09-01 00:00:00

abstract：:DNA replication forks pause in front of lesions on the template, eventually leading to cytotoxic chromosomal rearrangements. The in vivo structure of damaged eukaryotic replication intermediates has been so far elusive. Combining electron microscopy (EM) and two-dimensional (2D) gel electrophoresis, we found that UV-i...

journal_title：Molecular cell

authors： Lopes M,Foiani M,Sogo JM

更新日期：2006-01-06 00:00:00

abstract：:Phospholipase C (PLC) isozymes are directly activated by heterotrimeric G proteins and Ras-like GTPases to hydrolyze phosphatidylinositol 4,5-bisphosphate into the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. Although PLCs play central roles in myriad signaling cascades, the molecular details of ...

journal_title：Molecular cell

authors： Hicks SN,Jezyk MR,Gershburg S,Seifert JP,Harden TK,Sondek J

更新日期：2008-08-08 00:00:00

abstract：:Phase-separated states of proteins underlie ribonucleoprotein (RNP) granules and nuclear RNA-binding protein assemblies that may nucleate protein inclusions associated with neurodegenerative diseases. We report that the N-terminal low-complexity domain of the RNA-binding protein Fused in Sarcoma (FUS LC) is structural...

journal_title：Molecular cell

authors： Burke KA,Janke AM,Rhine CL,Fawzi NL

更新日期：2015-10-15 00:00:00

abstract：:The SMN complex assembles Sm cores on snRNAs, a key step in the biogenesis of snRNPs, the spliceosome's major components. Here, using SMN complex inhibitors identified by high-throughput screening and a ribo-proteomic strategy on formaldehyde crosslinked RNPs, we dissected this pathway in cells. We show that protein s...

journal_title：Molecular cell

authors： Yong J,Kasim M,Bachorik JL,Wan L,Dreyfuss G

更新日期：2010-05-28 00:00:00

abstract：:Activating mutations in NOTCH1, an essential regulator of T cell development, are frequently found in human T cell acute lymphoblastic leukemia (T-ALL). Despite important advances in our understanding of Notch signal transduction, the regulation of Notch functions in the nucleus remains unclear. Using immunoaffinity p...

journal_title：Molecular cell

authors： Yatim A,Benne C,Sobhian B,Laurent-Chabalier S,Deas O,Judde JG,Lelievre JD,Levy Y,Benkirane M

更新日期：2012-11-09 00:00:00

abstract：:Chaperone-mediated autophagy (CMA) is involved in cellular quality control, and in the response to stress, deterioration in CMA contributes to the aging phenotype and to various disorders. The paper by Bandyopadhyay et al. in this issue of Molecular Cell demonstrates the role of GTP binding by elongation factor 1 alph...

journal_title：Molecular cell

authors： Beau I,Codogno P

更新日期：2010-08-27 00:00:00

abstract：:Multicellular organisms have multiple homologs of the yeast ATG8 gene, but the differential roles of these homologs in autophagy during development remain largely unknown. Here we investigated structure/function relationships in the two C. elegans Atg8 homologs, LGG-1 and LGG-2. lgg-1 is essential for degradation of p...

journal_title：Molecular cell

authors： Wu F,Watanabe Y,Guo XY,Qi X,Wang P,Zhao HY,Wang Z,Fujioka Y,Zhang H,Ren JQ,Fang TC,Shen YX,Feng W,Hu JJ,Noda NN,Zhang H

更新日期：2015-12-17 00:00:00

abstract：:Eukaryotic cells compartmentalize biochemical processes in different organelles, often relying on metabolic cycles to shuttle reducing equivalents across intracellular membranes. NADPH serves as the electron carrier for the maintenance of redox homeostasis and reductive biosynthesis, with separate cytosolic and mitoch...

journal_title：Molecular cell

authors： Lewis CA,Parker SJ,Fiske BP,McCloskey D,Gui DY,Green CR,Vokes NI,Feist AM,Vander Heiden MG,Metallo CM

更新日期：2014-07-17 00:00:00

abstract：:The selective degradation of intracellular components by autophagy involves sequential interactions of the cargo with a receptor, which also binds the autophagosomal protein Atg8 and a scaffold protein. Here, we demonstrated that mutations in C. elegans epg-11, which encodes an arginine methyltransferase homologous to...

journal_title：Molecular cell

authors： Li S,Yang P,Tian E,Zhang H

更新日期：2013-11-07 00:00:00

abstract：:The phosphoinositide (PI)-3-kinase-related kinase (PIKK) family proteins Tel1p and Mec1p have been implicated in the telomere integrity of Saccharomyces cerevisiae. However, the mechanism of PIKK-mediated telomere length control remains unclear. Here, we show that Tel1p and Mec1p are recruited to the telomeres at spec...

journal_title：Molecular cell

authors： Takata H,Kanoh Y,Gunge N,Shirahige K,Matsuura A

更新日期：2004-05-21 00:00:00

abstract：:Polyubiquitin chains of different topologies regulate diverse cellular processes. K48- and K63-linked chains, the two most abundant chain types, regulate proteolytic and signaling pathways, respectively. Although recent studies reported important roles for heterogeneous chains, the functions of branched ubiquitin chai...

journal_title：Molecular cell

authors： Ohtake F,Saeki Y,Ishido S,Kanno J,Tanaka K

更新日期：2016-10-20 00:00:00

abstract：:Genome stability depends upon the RecQ helicases, which are conserved from bacteria to man, but little is known about how their myriad activities are regulated. Fission yeast lacking the telomere protein Taz1 (mammalian TRF1/TRF2 ortholog) lose many hallmarks of telomeres, including accurate replication and local prot...

journal_title：Molecular cell

authors： Rog O,Miller KM,Ferreira MG,Cooper JP

更新日期：2009-03-13 00:00:00

abstract：:Serine/arginine (SR)-rich splicing factors contain an RNA binding domain and an arginine/serine (RS)-rich domain required for protein-protein interactions. In addition to their roles in the basic splicing reaction, SR proteins function as components of splicing enhancer complexes. Here, we investigate the role of RS d...

journal_title：Molecular cell

authors： Graveley BR,Maniatis T

更新日期：1998-04-01 00:00:00

abstract：:MRE11 within the MRE11-RAD50-NBS1 (MRN) complex acts in DNA double-strand break repair (DSBR), detection, and signaling; yet, how its endo- and exonuclease activities regulate DSBR by nonhomologous end-joining (NHEJ) versus homologous recombination (HR) remains enigmatic. Here, we employed structure-based design with ...

journal_title：Molecular cell

authors： Shibata A,Moiani D,Arvai AS,Perry J,Harding SM,Genois MM,Maity R,van Rossum-Fikkert S,Kertokalio A,Romoli F,Ismail A,Ismalaj E,Petricci E,Neale MJ,Bristow RG,Masson JY,Wyman C,Jeggo PA,Tainer JA

更新日期：2014-01-09 00:00:00

abstract：:Mutations that lead to splicing defects can have severe consequences on gene function and cause disease. Here, we explore how human genetic variation affects exon recognition by developing a multiplexed functional assay of splicing using Sort-seq (MFASS). We assayed 27,733 variants in the Exome Aggregation Consortium ...

journal_title：Molecular cell

authors： Cheung R,Insigne KD,Yao D,Burghard CP,Wang J,Hsiao YE,Jones EM,Goodman DB,Xiao X,Kosuri S

更新日期：2019-01-03 00:00:00

abstract：:The orphan nuclear receptors SF-1 and LRH-1 are constitutively active, but it remains uncertain whether their activation is hormone dependent. We report the crystal structure of the LRH-1 ligand binding domain to 2.4 A resolution and find the receptor to be a monomer that adopts an active conformation with a large but...

journal_title：Molecular cell

authors： Sablin EP,Krylova IN,Fletterick RJ,Ingraham HA

更新日期：2003-06-01 00:00:00

abstract：:Perfringolysin O, a bacterial cytolytic toxin, forms unusually large pores in cholesterol-containing membranes by the spontaneous insertion of two of its four domains into the bilayer. By monitoring the kinetics of domain-specific conformational changes and pore formation using fluorescence spectroscopy, the temporal ...

journal_title：Molecular cell

authors： Heuck AP,Hotze EM,Tweten RK,Johnson AE

更新日期：2000-11-01 00:00:00

abstract：:Mammalian cells constantly monitor and respond to a myriad of extracellular signals, often by using cell surface receptors. Two important classes of cell surface receptors include the receptor tyrosine kinases, which recognize peptide growth factors such as insulin, and the integrins, which most often mediate binding ...

journal_title：Molecular cell

authors： Shrivastava A,Radziejewski C,Campbell E,Kovac L,McGlynn M,Ryan TE,Davis S,Goldfarb MP,Glass DJ,Lemke G,Yancopoulos GD

更新日期：1997-12-01 00:00:00