An acid test for g proteins.

abstract：:Bcl-2 proteins are critical regulators of apoptosis following DNA damage. Recent studies have shown that genotoxic stress induces the deamidation of Bcl-X(L), inhibiting its antiapoptotic activities. Remarkably, Rb and p53 are important regulators of this novel modification of Bcl-X(L). ...

journal_title：Molecular cell

authors： Johnstone RW

更新日期：2002-10-01 00:00:00

abstract：:In a recent paper in Developmental Cell, Yamashiro et al. (2008) report that the PP1 regulatory subunit MYPT1 interacts with PLK1 and antagonizes essential mitotic functions of PLK1, at least in part by promoting the dephosphorylation of PLK1 at Thr210. ...

journal_title：Molecular cell

authors： Archambault V,Chen F,Glover DM

更新日期：2008-06-06 00:00:00

abstract：:In this issue of Molecular Cell, Chereji et al. (2017) present new data on MNase-sensitive particles previously identified upstream of transcription start sites at many promoters in budding yeast, and they argue, based upon negative histone-ChIP results, that they are non-nucleosomal signals generated by transcription...

journal_title：Molecular cell

authors： Kubik S,Bruzzone MJ,Albert B,Shore D

更新日期：2017-02-02 00:00:00

abstract：:The crystal structure of a heterodimer between the ligand-binding domains (LBDs) of the human RARalpha bound to a selective antagonist and the constitutively active mouse RXRalphaF318A mutant shows that, pushed by a bulky extension of the ligand, RARalpha helix H12 adopts an antagonist position. The unexpected presenc...

journal_title：Molecular cell

authors： Bourguet W,Vivat V,Wurtz JM,Chambon P,Gronemeyer H,Moras D

更新日期：2000-02-01 00:00:00

abstract：:Anti-sense transcription originating upstream of mammalian protein-coding genes is a well-documented phenomenon, but remarkably little is known about the regulation or function of anti-sense promoters and the non-coding RNAs they generate. Here we define at nucleotide resolution the divergent transcription start sites...

journal_title：Molecular cell

authors： Scruggs BS,Gilchrist DA,Nechaev S,Muse GW,Burkholder A,Fargo DC,Adelman K

更新日期：2015-06-18 00:00:00

abstract：:RNA binding proteins (RBPs) regulate all aspects in the life cycle of RNA molecules. To elucidate the elements that guide RNA specificity, regulatory mechanisms, and functions of RBPs, methods that identify direct endogenous protein-RNA interactions are particularly valuable. UV crosslinking and immunoprecipitation (C...

journal_title：Molecular cell

authors： Lee FCY,Ule J

更新日期：2018-02-01 00:00:00

abstract：:Signal-transduction cascades are usually studied on cell averages, masking variability between individual cells. To address this, we studied in individual cells the dynamic response of ERK2, a well-characterized MAPK signaling protein, which enters the nucleus upon stimulation. Using fluorescent tagging at the endogen...

journal_title：Molecular cell

authors： Cohen-Saidon C,Cohen AA,Sigal A,Liron Y,Alon U

更新日期：2009-12-11 00:00:00

abstract：:Klein et al. (2020) demonstrate for the first time that small-molecule cancer therapeutics are selectively partitioned and concentrated within phase-separated nuclear condensates, providing new insights to drug efficacy and creating the opportunity for enhanced control of therapeutic targeting. ...

journal_title：Molecular cell

authors： Howard TP,Roberts CWM

更新日期：2020-08-20 00:00:00

abstract：:In this issue of Molecular Cell, Terrell et al. (2019) characterize the interactions of RAS-RAF complexes in live cells. Using bioluminescence resonance energy transfer (BRET) technology, they show that individual RAF family members exhibit distinct binding affinities to each RAS family protein, with CRAF exhibiting h...

journal_title：Molecular cell

authors： Venkatanarayan A,Malek S

更新日期：2019-12-19 00:00:00

abstract：:In eukaryotes, a crucial step of translation initiation is the binding of the multifactor complex eIF4F to the 5' end of the mRNA, a prerequisite to recruitment of the activated small ribosomal 43S particle. Histone H4 mRNAs have short 5'UTRs, which do not conform to the conventional scanning-initiation model. Here we...

journal_title：Molecular cell

authors： Martin F,Barends S,Jaeger S,Schaeffer L,Prongidi-Fix L,Eriani G

更新日期：2011-01-21 00:00:00

abstract：:While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combin...

journal_title：Molecular cell

authors： Douangamath A,Filipp FV,Klein AT,Barnett P,Zou P,Voorn-Brouwer T,Vega MC,Mayans OM,Sattler M,Distel B,Wilmanns M

更新日期：2002-11-01 00:00:00

abstract：:The Latin word "facultas" literally means "opportunity." Facultative heterochromatin (fHC) then designates genomic regions in the nucleus of a eukaryotic cell that have the opportunity to adopt open or compact conformations within temporal and spatial contexts. This review focuses on the molecular and functional aspec...

journal_title：Molecular cell

authors： Trojer P,Reinberg D

更新日期：2007-10-12 00:00:00

abstract：:The identification of metazoan origins of DNA replication has so far been hampered by the lack of a suitable genetic screening and by the cumbersomeness of the currently available mapping procedures. Here we describe the construction of a library of nascent DNA, representative of all cellular origin sequences, and its...

journal_title：Molecular cell

authors： Todorovic V,Giadrossi S,Pelizon C,Mendoza-Maldonado R,Masai H,Giacca M

更新日期：2005-08-19 00:00:00

abstract：:In a recent paper in Cell Metabolism, Altman et al. (2015) report that MYC disrupts the molecular clock in cancer cells and describe a link between oncogenesis, circadian rhythms, and metabolism. ...

journal_title：Molecular cell

authors： Lau AN,Vander Heiden MG

更新日期：2015-11-19 00:00:00

abstract：:The heterodimeric nuclear cap binding complex (CBC) binds to 5'-capped polymerase II transcripts. It enhances the efficiency of several mRNA maturation steps and is essential for U snRNA nuclear export in multicellular eukaryotes. The 2A crystal structure of human CBC shows that the large subunit, CBP80, comprises thr...

journal_title：Molecular cell

authors： Mazza C,Ohno M,Segref A,Mattaj IW,Cusack S

更新日期：2001-08-01 00:00:00

abstract：:To map the genomic interaction sites of chromatin proteins, two related methods were developed and experimentally explored in Saccharomyces cerevisiae. The ChIC method (chromatin immunocleavage) consists of tethering a fusion protein (pA-MN) consisting of micrococcal nuclease (MN) and staphylococcal protein A to speci...

journal_title：Molecular cell

authors： Schmid M,Durussel T,Laemmli UK

更新日期：2004-10-08 00:00:00

abstract：:Ubiquitin-mediated proteolysis regulates the activity of diverse receptor systems. Here, we identify Smurf2, a C2-WW-HECT domain ubiquitin ligase and show that Smurf2 associates constitutively with Smad7. Smurf2 is nuclear, but binding to Smad7 induces export and recruitment to the activated TGF beta receptor, where i...

journal_title：Molecular cell

authors： Kavsak P,Rasmussen RK,Causing CG,Bonni S,Zhu H,Thomsen GH,Wrana JL

更新日期：2000-12-01 00:00:00

abstract：:Ten E. coli aminoacyl-tRNAs (aa-tRNAs) were assessed for their ability to decode cognate codons on E. coli ribosomes by using three assays that evaluate the key steps in the decoding pathway. Despite a wide variety of structural features, each aa-tRNA exhibited similar kinetic and thermodynamic properties in each assa...

journal_title：Molecular cell

authors： Ledoux S,Uhlenbeck OC

更新日期：2008-07-11 00:00:00

abstract：:We have identified human MBT domain-containing protein L3MBTL2 as an integral component of a protein complex that we termed Polycomb repressive complex 1 (PRC1)-like 4 (PRC1L4), given the copresence of PcG proteins RING1, RING2, and PCGF6/MBLR. PRC1L4 also contained E2F6 and CBX3/HP1γ, known to function in transcripti...

journal_title：Molecular cell

authors： Trojer P,Cao AR,Gao Z,Li Y,Zhang J,Xu X,Li G,Losson R,Erdjument-Bromage H,Tempst P,Farnham PJ,Reinberg D

更新日期：2011-05-20 00:00:00

abstract：:Two cyclin-dependent kinases have been identified in yeast and mammalian RNA polymerase II transcription initiation complexes. We find that the two yeast kinases are indistinguishable in their ability to phosphorylate the RNA polymerase II CTD, and yet in living cells one kinase is a positive regulator and the other a...

journal_title：Molecular cell

authors： Hengartner CJ,Myer VE,Liao SM,Wilson CJ,Koh SS,Young RA

更新日期：1998-07-01 00:00:00

abstract：:Nine human neurodegenerative diseases are due to expansion of a CAG repeat- encoding glutamine within the open reading frame of the respective genes. Polyglutamine (polyQ) expansion confers dominant toxicity, resulting in neuronal degeneration. MicroRNAs (miRNAs) have been shown to modulate programmed cell death durin...

journal_title：Molecular cell

authors： Bilen J,Liu N,Burnett BG,Pittman RN,Bonini NM

更新日期：2006-10-06 00:00:00

abstract：:ClpXP is a protease involved in DNA damage repair, stationary-phase gene expression, and ssrA-mediated protein quality control. To date, however, only a handful of ClpXP substrates have been identified. Using a tagged and inactive variant of ClpP, substrates of E. coli ClpXP were trapped in vivo, purified, and identif...

journal_title：Molecular cell

authors： Flynn JM,Neher SB,Kim YI,Sauer RT,Baker TA

更新日期：2003-03-01 00:00:00

abstract：:An important paper by David Allis and colleagues (Ahn et al., 2006 [this issue of Molecular Cell]) describes a specific deacetylation/phosphorylation crosstalk at the histone H2B tail required for apoptosis induction in yeast, thus giving first insights into the poorly understood epigenetic regulation of cell death. ...

journal_title：Molecular cell

authors： Carmona-Gutierrez D,Madeo F

更新日期：2006-10-20 00:00:00

abstract：:The rtpA gene of Bacillus subtilis encodes the Anti-TRAP protein, AT. AT can bind and inhibit the TRAP regulatory protein, preventing TRAP from promoting transcription termination in the trpEDCFBA operon leader region. AT synthesis is upregulated transcriptionally and translationally in response to the accumulation of...

journal_title：Molecular cell

authors： Chen G,Yanofsky C

更新日期：2004-03-12 00:00:00

abstract：:One of the prominent biological phenomena controlled by Wnt signaling is the expansion of cells with predefined fates. In the November 27, 2002, issue of Cell, Rosenfeld and colleagues describe an example of Wnt-controlled proliferation during genesis of the cardiac outflow tract and the pituitary gland. As a downstre...

journal_title：Molecular cell

authors： Clevers H

更新日期：2002-12-01 00:00:00

abstract：:The control of mRNA stability plays a central role in regulating gene expression. In metazoans, the earliest stages of development are driven by maternally supplied mRNAs. The degradation of these maternal mRNAs is critical for promoting the maternal-to-zygotic transition of developmental programs, although the underl...

journal_title：Molecular cell

authors： Mishima Y,Tomari Y

更新日期：2016-03-17 00:00:00

abstract：:Interference with nucleocytoplasmic transport is a strategy employed by certain viruses to compromise host cellular function. While it has been shown that the matrix (M) protein of the vesicular stomatitis virus (VSV) inhibits nuclear export of host cell mRNAs, the underlying mechanism has not been fully established. ...

journal_title：Molecular cell

authors： Faria PA,Chakraborty P,Levay A,Barber GN,Ezelle HJ,Enninga J,Arana C,van Deursen J,Fontoura BM

更新日期：2005-01-07 00:00:00

abstract：:miRNAs are largely known to base pair with the 3'UTR of target mRNAs, downregulating their stability and translation. mRNA of betaTrCP1 ubiquitin ligase is very unstable, but unlike the majority of mRNAs where 3'UTR determines the rate of mRNA turnover, betaTrCP1 mRNA contains cis-acting destabilizing elements within ...

journal_title：Molecular cell

authors： Elcheva I,Goswami S,Noubissi FK,Spiegelman VS

更新日期：2009-07-31 00:00:00

abstract：:Recently, a novel family of TATA binding protein (TBP)-like factors (TLFs) have been described in metazoan organisms; however, their function has not yet been elucidated. Using Caenorhabditis elegans (Ce) as a model, we demonstrate that CeTLF is required in vivo for zygotic transcription during embryogenesis. Eliminat...

journal_title：Molecular cell

authors： Dantonel JC,Quintin S,Lakatos L,Labouesse M,Tora L

更新日期：2000-09-01 00:00:00

abstract：:Activation of NF-kappaB is regulated via numerous ubiquitin- and proteasome-mediated steps; an important one is processing of the precursor p105 to the p50 active subunit. The mechanisms involved are largely unknown, because this is an exceptional case where the ubiquitin system does not destroy its substrate complete...

journal_title：Molecular cell

authors： Kravtsova-Ivantsiv Y,Cohen S,Ciechanover A

更新日期：2009-02-27 00:00:00