Abstract：
:Serine/arginine (SR)-rich splicing factors contain an RNA binding domain and an arginine/serine (RS)-rich domain required for protein-protein interactions. In addition to their roles in the basic splicing reaction, SR proteins function as components of splicing enhancer complexes. Here, we investigate the role of RS domains in splicing enhancer function. Hybrid proteins containing RS domains fused to the MS2 RNA binding protein were tested in vitro with RNA substrates bearing an MS2 recognition sequence. These hybrid proteins activated splicing in nuclear extracts, but not in S100 extracts lacking SR proteins. However, intact recombinant SR proteins could complement the activity of the hybrid proteins in S100 extracts. These data demonstrate that RS domains function as splicing activators and suggest that the general and enhancer-dependent functions of SR proteins can be uncoupled.
journal_name
Mol Celljournal_title
Molecular cellauthors
Graveley BR,Maniatis Tdoi
10.1016/s1097-2765(00)80076-3subject
Has Abstractpub_date
1998-04-01 00:00:00pages
765-71issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80076-3journal_volume
1pub_type
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