DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities.

abstract：:During muscle regeneration, the mechanism integrating environmental cues at the chromatin of muscle progenitors is unknown. We show that inflammation-activated MKK6-p38 and insulin growth factor 1 (IGF1)-induced PI3K/AKT pathways converge on the chromatin of muscle genes to target distinct components of the muscle tra...

journal_title：Molecular cell

authors： Serra C,Palacios D,Mozzetta C,Forcales SV,Morantte I,Ripani M,Jones DR,Du K,Jhala US,Simone C,Puri PL

更新日期：2007-10-26 00:00:00

abstract：:Molecular motors are the long-haul carriers of eukaryotic cells, moving cargos bidirectionally along microtubule tracks. In the December 12th issue of Cell, report that HDAC6, a tubulin deacetylase, functions as an adaptor that links cargos of aggregated protein to the minus end-directed motor, cytoplasmic dynein. ...

journal_title：Molecular cell

authors： Kopito RR

更新日期：2003-12-01 00:00:00

abstract：:Ubiquitination typically occurs through the sequential action of three enzymes catalyzing ubiquitin activation (E1), conjugation (E2), and ligation (E3) and regulates diverse eukaryotic cellular processes. Although monoubiquitination commonly confers nondegradative activities, mechanisms underlying its temporal and sp...

journal_title：Molecular cell

authors： Sun L,Fang J

更新日期：2016-06-16 00:00:00

abstract：:Cdh1 is a well-established activator of APC/C, a RING-type ubiquitin ligase. In this issue of Molecular Cell, Wan et al. (2011) report an APC/C-independent role of Cdh1 during development as an activator for Smurf1, a HECT-type ubiquitin ligase. ...

journal_title：Molecular cell

authors： Jia L,Yu H

更新日期：2011-12-09 00:00:00

abstract：:Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Unbiased detection through proximity labeling ...

journal_title：Molecular cell

authors： Wang AS,Chen LC,Wu RA,Hao Y,McSwiggen DT,Heckert AB,Richardson CD,Gowen BG,Kazane KR,Vu JT,Wyman SK,Shin JJ,Darzacq X,Walter JC,Corn JE

更新日期：2020-07-16 00:00:00

abstract：:PTPA, an essential and specific activator of protein phosphatase 2A (PP2A), functions as a peptidyl prolyl isomerase (PPIase). We present here the crystal structures of human PTPA and of the two yeast orthologs (Ypa1 and Ypa2), revealing an all alpha-helical protein fold that is radically different from other PPIases....

journal_title：Molecular cell

authors： Leulliot N,Vicentini G,Jordens J,Quevillon-Cheruel S,Schiltz M,Barford D,van Tilbeurgh H,Goris J

更新日期：2006-08-04 00:00:00

abstract：:The proteolytic activation of the membrane-associated transcription factor pro-sigma(K) is controlled by a signal transduction pathway during sporulation in the bacterium Bacillus subtilis. The pro-sigma(K) processing enzyme SpoIVFB, a membrane-embedded metalloprotease, is held inactive by two other integral-membrane ...

journal_title：Molecular cell

authors： Campo N,Rudner DZ

更新日期：2006-07-07 00:00:00

abstract：:RNA molecules play diverse functional roles in natural biological systems. There has been growing interest in designing synthetic RNA counterparts for programming biological function. The design of synthetic RNA molecules that exhibit diverse activities, including sensing, regulatory, information processing, and scaff...

journal_title：Molecular cell

authors： Liang JC,Bloom RJ,Smolke CD

更新日期：2011-09-16 00:00:00

abstract：:The role of the protein kinase Akt in cell migration is incompletely understood. Here we show that sphingosine-1-phosphate (S1P)-induced endothelial cell migration requires the Akt-mediated phosphorylation of the G protein-coupled receptor (GPCR) EDG-1. Activated Akt binds to EDG-1 and phosphorylates the third intrace...

journal_title：Molecular cell

authors： Lee MJ,Thangada S,Paik JH,Sapkota GP,Ancellin N,Chae SS,Wu M,Morales-Ruiz M,Sessa WC,Alessi DR,Hla T

更新日期：2001-09-01 00:00:00

abstract：:One of the prominent biological phenomena controlled by Wnt signaling is the expansion of cells with predefined fates. In the November 27, 2002, issue of Cell, Rosenfeld and colleagues describe an example of Wnt-controlled proliferation during genesis of the cardiac outflow tract and the pituitary gland. As a downstre...

journal_title：Molecular cell

authors： Clevers H

更新日期：2002-12-01 00:00:00

abstract：:Unscheduled RNA-DNA hybrids promote the formation DNA double-strand breaks (DSBs) through poorly characterized mechanism. In this issue, Sollier et al. (2014) show that R-loops are processed by XPF and XPG, two structure-specific endonucleases of the NER pathway, to generate DSBs. ...

journal_title：Molecular cell

authors： Lin YL,Pasero P

更新日期：2014-12-18 00:00:00

abstract：:In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied ini...

journal_title：Molecular cell

authors： Duchi D,Bauer DL,Fernandez L,Evans G,Robb N,Hwang LC,Gryte K,Tomescu A,Zawadzki P,Morichaud Z,Brodolin K,Kapanidis AN

更新日期：2016-09-15 00:00:00

abstract：:Tethering of synaptic vesicles (SVs) to the active zone determines synaptic strength, although the molecular basis governing SV tethering is elusive. Here, we discover that small unilamellar vesicles (SUVs) and SVs from rat brains coat on the surface of condensed liquid droplets formed by active zone proteins RIM, RIM...

journal_title：Molecular cell

authors： Wu X,Ganzella M,Zhou J,Zhu S,Jahn R,Zhang M

更新日期：2021-01-07 00:00:00

abstract：:In this issue, Roberts et al. (2014) describe how hexokinase and mTORC1 cooperate to sense disequilibrium between glucose uptake and utilization and direct the balance of anabolism and catabolism to ensure the appropriate use of cellular resources. ...

journal_title：Molecular cell

authors： Kundu M

更新日期：2014-02-20 00:00:00

abstract：:A defining feature of centromeres is the presence of the histone H3 variant CENP-A(Cnp1). It is not known how CENP-A(Cnp1) is specifically delivered to, and assembled into, centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast, we identified Sim3. Sim3 is homologous to ...

journal_title：Molecular cell

authors： Dunleavy EM,Pidoux AL,Monet M,Bonilla C,Richardson W,Hamilton GL,Ekwall K,McLaughlin PJ,Allshire RC

更新日期：2007-12-28 00:00:00

abstract：:In response to hyperosmotic environments, most eukaryotic cells activate a specialized mitogen-activated protein (MAP) kinase pathway. In S. cerevisiae, the key protein kinase, Hog1, coordinates the transcriptional induction of a variety of genes devoted to osmoadaptation and general stress protection. Depending on th...

journal_title：Molecular cell

authors： Alepuz PM,Jovanovic A,Reiser V,Ammerer G

更新日期：2001-04-01 00:00:00

abstract：:B cell CLL/lymphoma-2 (BCL-2) and its relatives comprise the BCL-2 family of proteins, which were originally characterized with respect to their roles in controlling outer mitochondrial membrane integrity and apoptosis. Current observations expand BCL-2 family function to include numerous cellular pathways. Here we wi...

journal_title：Molecular cell

authors： Chipuk JE,Moldoveanu T,Llambi F,Parsons MJ,Green DR

更新日期：2010-02-12 00:00:00

abstract：:The C-terminal fragment, Bb, of factor B combines with C3b to form the pivotal C3-convertase, C3bBb, of alternative complement pathway. Bb consists of a von Willebrand factor type A (vWFA) domain that is structurally similar to the I domains of integrins and a serine protease (SP) domain that is in inactive conformati...

journal_title：Molecular cell

authors： Ponnuraj K,Xu Y,Macon K,Moore D,Volanakis JE,Narayana SV

更新日期：2004-04-09 00:00:00

abstract：:The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca(2+)] changes ([Ca(2+)](ER-mt)) using drug-inducible fluorescent interorg...

journal_title：Molecular cell

authors： Csordás G,Várnai P,Golenár T,Roy S,Purkins G,Schneider TG,Balla T,Hajnóczky G

更新日期：2010-07-09 00:00:00

abstract：:Transcription and splicing are functionally coupled, resulting in highly efficient splicing of RNA polymerase II (RNAP II) transcripts. The mechanism involved in this coupling is not known. To identify potential coupling factors, we carried out a comprehensive proteomic analysis of immunopurified human RNAP II, identi...

journal_title：Molecular cell

authors： Das R,Yu J,Zhang Z,Gygi MP,Krainer AR,Gygi SP,Reed R

更新日期：2007-06-22 00:00:00

abstract：:Cells exposed to hypoxia experience replication stress but do not accumulate DNA damage, suggesting sustained DNA replication. Ribonucleotide reductase (RNR) is the only enzyme capable of de novo synthesis of deoxyribonucleotide triphosphates (dNTPs). However, oxygen is an essential cofactor for mammalian RNR (RRM1/RR...

journal_title：Molecular cell

authors： Foskolou IP,Jorgensen C,Leszczynska KB,Olcina MM,Tarhonskaya H,Haisma B,D'Angiolella V,Myers WK,Domene C,Flashman E,Hammond EM

更新日期：2017-04-20 00:00:00

abstract：:N6-methyladenosine (m6A) and adenosine-to-inosine (A-to-I) editing are two of the most abundant RNA modifications, both at adenosines. Yet, the interaction of these two types of adenosine modifications is largely unknown. Here we show a global A-to-I difference between m6A-positive and m6A-negative RNA populations. Bo...

journal_title：Molecular cell

authors： Xiang JF,Yang Q,Liu CX,Wu M,Chen LL,Yang L

更新日期：2018-01-04 00:00:00

abstract：:Two cyclin-dependent kinases have been identified in yeast and mammalian RNA polymerase II transcription initiation complexes. We find that the two yeast kinases are indistinguishable in their ability to phosphorylate the RNA polymerase II CTD, and yet in living cells one kinase is a positive regulator and the other a...

journal_title：Molecular cell

authors： Hengartner CJ,Myer VE,Liao SM,Wilson CJ,Koh SS,Young RA

更新日期：1998-07-01 00:00:00

abstract：:Steroid hormones have rapid nongenomic effects on cell-signaling pathways, but the receptor mechanisms responsible for this are not understood. We have identified a specific polyproline motif in the amino-terminal domain of conventional progesterone receptor (PR) that mediates direct progestin-dependent interaction of...

journal_title：Molecular cell

authors： Boonyaratanakornkit V,Scott MP,Ribon V,Sherman L,Anderson SM,Maller JL,Miller WT,Edwards DP

更新日期：2001-08-01 00:00:00

abstract：:Spt6 is an essential histone chaperone that mediates nucleosome reassembly during gene transcription. Spt6 also associates with RNA polymerase II (RNAPII) via a tandem Src2 homology domain. However, the significance of Spt6-RNAPII interaction is not well understood. Here, we show that Spt6 recruitment to genes and the...

journal_title：Molecular cell

authors： Dronamraju R,Hepperla AJ,Shibata Y,Adams AT,Magnuson T,Davis IJ,Strahl BD

更新日期：2018-06-21 00:00:00

abstract：:Physical and genetic studies verify that the DNA binding domain of protein gpNu1 (which initiates packaging of phage lambda DNA) is a winged helix-turn-helix (w HTH) and that gpNu1 dimers bind sites that are brought close through DNA bending. ...

journal_title：Molecular cell

authors： Campbell A

更新日期：2002-05-01 00:00:00

abstract：:RING and U-box E3 ubiquitin ligases regulate diverse eukaryotic processes and have been implicated in numerous diseases, but targeting these enzymes remains a major challenge. We report the development of three ubiquitin variants (UbVs), each binding selectively to the RING or U-box domain of a distinct E3 ligase: mon...

journal_title：Molecular cell

authors： Gabrielsen M,Buetow L,Nakasone MA,Ahmed SF,Sibbet GJ,Smith BO,Zhang W,Sidhu SS,Huang DT

更新日期：2017-10-19 00:00:00

abstract：:Serine-arginine (SR) proteins are general splicing factors and can function through binding to exonic splicing enhancers (ESEs). SR proteins and several other mammalian splicing factors contain an arginine-serine-rich (RS) domain required to promote splicing. We have recently found that the ESE bound RS domain functio...

journal_title：Molecular cell

authors： Shen H,Green MR

更新日期：2004-11-05 00:00:00

abstract：:RNA binding proteins (RBPs) regulate all aspects in the life cycle of RNA molecules. To elucidate the elements that guide RNA specificity, regulatory mechanisms, and functions of RBPs, methods that identify direct endogenous protein-RNA interactions are particularly valuable. UV crosslinking and immunoprecipitation (C...

journal_title：Molecular cell

authors： Lee FCY,Ule J

更新日期：2018-02-01 00:00:00

abstract：:Protein kinase B/Akt plays crucial roles in promoting cell survival and mediating insulin responses. The enzyme is stimulated by phosphorylation at two regulatory sites: Thr 309 of the activation segment and Ser 474 of the hydrophobic motif, a conserved feature of many AGC kinases. Analysis of the crystal structures o...

journal_title：Molecular cell

authors： Yang J,Cron P,Thompson V,Good VM,Hess D,Hemmings BA,Barford D

更新日期：2002-06-01 00:00:00