Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases.

abstract：:Herpes simplex virus (HSV) infection requires binding of the viral envelope glycoprotein D (gD) to cell surface receptors. We report the X-ray structures of a soluble, truncated ectodomain of gD both alone and in complex with the ectodomain of its cellular receptor HveA. Two bound anions suggest possible binding sites...

journal_title：Molecular cell

authors： Carfí A,Willis SH,Whitbeck JC,Krummenacher C,Cohen GH,Eisenberg RJ,Wiley DC

更新日期：2001-07-01 00:00:00

abstract：:Tumor suppressor genes evolved as negative effectors of mitogen and nutrient signaling pathways, such that mutations in these genes can lead to pathological states of growth. Tuberous sclerosis (TSC) is a potentially devastating disease associated with mutations in two tumor suppressor genes, TSC1 and 2, that function...

journal_title：Molecular cell

authors： Garami A,Zwartkruis FJ,Nobukuni T,Joaquin M,Roccio M,Stocker H,Kozma SC,Hafen E,Bos JL,Thomas G

更新日期：2003-06-01 00:00:00

abstract：:Homologous recombination (HR) is crucial for genetic exchange and accurate repair of DNA double-strand breaks and is pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly und...

journal_title：Molecular cell

authors： Renkawitz J,Lademann CA,Kalocsay M,Jentsch S

更新日期：2013-04-25 00:00:00

abstract：:The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor contro...

journal_title：Molecular cell

authors： He C,Hus JC,Sun LJ,Zhou P,Norman DP,Dötsch V,Wei H,Gross JD,Lane WS,Wagner G,Verdine GL

更新日期：2005-10-07 00:00:00

abstract：:Transcriptional pausing by RNA polymerase (RNAP) plays an essential role in gene regulation. Pausing is modified by various elongation factors, including prokaryotic NusA, but the mechanisms underlying pausing and NusA function remain unclear. Alternative models for pausing invoke blockade events that precede transloc...

journal_title：Molecular cell

authors： Zhou J,Ha KS,La Porta A,Landick R,Block SM

更新日期：2011-11-18 00:00:00

abstract：:The orphan nuclear receptors SF-1 and LRH-1 are constitutively active, but it remains uncertain whether their activation is hormone dependent. We report the crystal structure of the LRH-1 ligand binding domain to 2.4 A resolution and find the receptor to be a monomer that adopts an active conformation with a large but...

journal_title：Molecular cell

authors： Sablin EP,Krylova IN,Fletterick RJ,Ingraham HA

更新日期：2003-06-01 00:00:00

abstract：:The pre-mRNA 5' splice site is recognized by the ACAGA box of U6 spliceosomal RNA prior to catalysis of splicing. We previously identified a mutant U4 spliceosomal RNA, U4-cs1, that masks the ACAGA box in the U4/U6 complex, thus conferring a cold-sensitive splicing phenotype in vivo. Here, we show that U4-cs1 blocks i...

journal_title：Molecular cell

authors： Kuhn AN,Li Z,Brow DA

更新日期：1999-01-01 00:00:00

abstract：:How repeating striped patterns arise across cellular fields is unclear. To address this we examined the repeating pattern of Stripe (Sr) expression across the parasegment (PS) in Drosophila. This pattern is generated in two steps. First, the ligands Hedgehog (Hh) and Wingless (Wg) subdivide the PS into smaller territo...

journal_title：Molecular cell

authors： Hatini V,DiNardo S

更新日期：2001-01-01 00:00:00

abstract：:YAP/TEAD are nuclear effectors of the Hippo pathway, regulating organ size and tumorigenesis largely through promoter-associated function. However, their function as enhancer regulators remains poorly understood. Through an in vivo proximity-dependent labeling (BioID) technique, we identified YAP1 and TEAD4 protein as...

journal_title：Molecular cell

authors： Zhu C,Li L,Zhang Z,Bi M,Wang H,Su W,Hernandez K,Liu P,Chen J,Chen M,Huang TH,Chen L,Liu Z

更新日期：2019-08-22 00:00:00

abstract：:Trimethylation of histone H3 on lysine 4 (H3K4me3) localizes near the 5' region of genes and is tightly associated with active loci. Several proteins, such as CHD1, BPTF, JMJD2A, and the ING tumor suppressor family, directly recognize this lysine methyl mark. However, how H3K4me3 recognition participates in active tra...

journal_title：Molecular cell

authors： Sims RJ 3rd,Millhouse S,Chen CF,Lewis BA,Erdjument-Bromage H,Tempst P,Manley JL,Reinberg D

更新日期：2007-11-30 00:00:00

abstract：:When faced with proteotoxic stress, cells mount adaptive responses to eliminate aberrant proteins. Adaptive responses increase the expression of protein folding and degradation factors to enhance the cellular quality control machinery. However, it is unclear whether and how this augmented machinery acquires new activi...

journal_title：Molecular cell

authors： Szoradi T,Schaeff K,Garcia-Rivera EM,Itzhak DN,Schmidt RM,Bircham PW,Leiss K,Diaz-Miyar J,Chen VK,Muzzey D,Borner GHH,Schuck S

更新日期：2018-06-21 00:00:00

abstract：:The mechanisms contributing to transcription-associated genomic instability are both complex and incompletely understood. Although R-loops are normal transcriptional intermediates, they are also associated with genomic instability. Here, we show that BRCA1 is recruited to R-loops that form normally over a subset of tr...

journal_title：Molecular cell

authors： Hatchi E,Skourti-Stathaki K,Ventz S,Pinello L,Yen A,Kamieniarz-Gdula K,Dimitrov S,Pathania S,McKinney KM,Eaton ML,Kellis M,Hill SJ,Parmigiani G,Proudfoot NJ,Livingston DM

更新日期：2015-02-19 00:00:00

abstract：:As solid tumors expand, oxygen and nutrients become limiting owing to inadequate vascularization and diffusion. How malignant cells cope with this potentially lethal metabolic stress remains poorly understood. We found that glucose shortage associated with malignant progression triggers apoptosis through the endoplasm...

journal_title：Molecular cell

authors： Huber AL,Lebeau J,Guillaumot P,Pétrilli V,Malek M,Chilloux J,Fauvet F,Payen L,Kfoury A,Renno T,Chevet E,Manié SN

更新日期：2013-03-28 00:00:00

abstract：:The heat shock protein 90 (Hsp90) family of heat shock proteins is an abundantly expressed and highly conserved family of ATP-dependent molecular chaperones. Hsp90 facilitates remodeling and activation of hundreds of proteins. In this study, we developed a screen to identify Hsp90-defective mutants in E. coli. The mut...

journal_title：Molecular cell

authors： Genest O,Reidy M,Street TO,Hoskins JR,Camberg JL,Agard DA,Masison DC,Wickner S

更新日期：2013-02-07 00:00:00

abstract：:Inappropriate activation of oncogenic kinases at intracellular locations is frequently observed in human cancers, but its effects on global signaling are incompletely understood. Here, we show that the oncogenic mutant of Flt3 (Flt3-ITD), when localized at the endoplasmic reticulum (ER), aberrantly activates STAT5 and...

journal_title：Molecular cell

authors： Choudhary C,Olsen JV,Brandts C,Cox J,Reddy PN,Böhmer FD,Gerke V,Schmidt-Arras DE,Berdel WE,Müller-Tidow C,Mann M,Serve H

更新日期：2009-10-23 00:00:00

abstract：:Molecular motors are the long-haul carriers of eukaryotic cells, moving cargos bidirectionally along microtubule tracks. In the December 12th issue of Cell, report that HDAC6, a tubulin deacetylase, functions as an adaptor that links cargos of aggregated protein to the minus end-directed motor, cytoplasmic dynein. ...

journal_title：Molecular cell

authors： Kopito RR

更新日期：2003-12-01 00:00:00

abstract：:Networks of protein interactions coordinate cellular functions. We describe a bimolecular fluorescence complementation (BiFC) assay for determination of the locations of protein interactions in living cells. This approach is based on complementation between two nonfluorescent fragments of the yellow fluorescent protei...

journal_title：Molecular cell

authors： Hu CD,Chinenov Y,Kerppola TK

更新日期：2002-04-01 00:00:00

abstract：:R-loop, a three-stranded RNA/DNA structure, has been linked to induced genome instability and regulated gene expression. To enable precision analysis of R-loops in vivo, we develop an RNase-H-based approach; this reveals predominant R-loop formation near gene promoters with strong G/C skew and propensity to form G-qua...

journal_title：Molecular cell

authors： Chen L,Chen JY,Zhang X,Gu Y,Xiao R,Shao C,Tang P,Qian H,Luo D,Li H,Zhou Y,Zhang DE,Fu XD

更新日期：2017-11-16 00:00:00

abstract：:We used DNA microarrays to profile gene expression patterns in the C. elegans germline and identified 1416 germline-enriched transcripts that define three groups. The sperm-enriched group contains an unusually large number of protein kinases and phosphatases. The oocyte-enriched group includes potentially new componen...

journal_title：Molecular cell

authors： Reinke V,Smith HE,Nance J,Wang J,Van Doren C,Begley R,Jones SJ,Davis EB,Scherer S,Ward S,Kim SK

更新日期：2000-09-01 00:00:00

abstract：:Alternative splicing (AS) is a widespread process underlying the generation of transcriptomic and proteomic diversity and is frequently misregulated in human disease. Accordingly, an important goal of biomedical research is the development of tools capable of comprehensively, accurately, and efficiently profiling AS. ...

journal_title：Molecular cell

authors： Sterne-Weiler T,Weatheritt RJ,Best AJ,Ha KCH,Blencowe BJ

更新日期：2018-10-04 00:00:00

abstract：:In this issue, Song et al. (2014) describe how microtubules and the RAN GTPase/importin-β system collaborate to control timing of HURP action and its turnover via the anaphase promoting complex (APC)-proteasome system, thereby ensuring temporal control of late mitotic events. ...

journal_title：Molecular cell

authors： Vaites LL,Harper JW

更新日期：2014-01-23 00:00:00

abstract：:The transition from transcription initiation to elongation at promoters of primary response genes (PRGs) in metazoan cells is controlled by inducible transcription factors, which utilize P-TEFb to phosphorylate RNA polymerase II (Pol II) in response to stimuli. Prior to stimulation, a fraction of P-TEFb is recruited t...

journal_title：Molecular cell

authors： McNamara RP,Reeder JE,McMillan EA,Bacon CW,McCann JL,D'Orso I

更新日期：2016-01-07 00:00:00

abstract：:Bcr-Abl is a dysregulated tyrosine kinase whose mechanism of activation is unclear. Here, we demonstrate that, like c-Abl, Bcr-Abl is negatively regulated through its SH3 domain. Kinase activity, transformation, and leukemogenesis by Bcr-Abl are greatly impaired by mutations of the Bcr coiled-coil domain that disrupt ...

journal_title：Molecular cell

authors： Smith KM,Yacobi R,Van Etten RA

更新日期：2003-07-01 00:00:00

abstract：:The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes through formation of heterocomplexes with p50 or p52. BCL-3 is phosphorylated in vivo, but specific BCL-3 kinases have not been identified so far. In this report, we show that BCL-3 is a substrate for the protein kinase GSK3 ...

journal_title：Molecular cell

authors： Viatour P,Dejardin E,Warnier M,Lair F,Claudio E,Bureau F,Marine JC,Merville MP,Maurer U,Green D,Piette J,Siebenlist U,Bours V,Chariot A

更新日期：2004-10-08 00:00:00

abstract：:In Drosophila, specification of embryonic terminal cells is controlled by the Torso receptor tyrosine kinase. Here, we analyze the molecular basis of positive (Y630) and negative (Y918) phosphotyrosine (pY) signaling sites on Torso. We find that the Drosophila homolog of RasGAP associates with pY918 and is a negative ...

journal_title：Molecular cell

authors： Cleghon V,Feldmann P,Ghiglione C,Copeland TD,Perrimon N,Hughes DA,Morrison DK

更新日期：1998-12-01 00:00:00

abstract：:CREB-binding protein (CBP) possesses an intrinsic acetyltransferase activity capable of acetylating nucleosomal histones as well as several nonhistone proteins. Here, it is shown that CBP can acetylate hepatocyte nuclear factor-4 (HNF-4), a member of the nuclear hormone receptor family, at lysine residues within the n...

journal_title：Molecular cell

authors： Soutoglou E,Katrakili N,Talianidis I

更新日期：2000-04-01 00:00:00

abstract：:Bicoid (BCD), the anterior determinant of Drosophila, controls embryonic gene expression by transcriptional activation and translational repression. Both functions require the homeodomain (HD), which recognizes DNA motifs at target gene enhancers and a specific sequence interval in the 3' untranslated region of caudal...

journal_title：Molecular cell

authors： Niessing D,Driever W,Sprenger F,Taubert H,Jäckle H,Rivera-Pomar R

更新日期：2000-02-01 00:00:00

abstract：:The redundancy of the genetic code implies that most amino acids are encoded by multiple synonymous codons. In all domains of life, a biased frequency of synonymous codons is observed at the genome level, in functionally related genes (e.g., in operons), and within single genes. Other codon bias variants include biase...

journal_title：Molecular cell

authors： Quax TE,Claassens NJ,Söll D,van der Oost J

更新日期：2015-07-16 00:00:00

abstract：:MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without ...

journal_title：Molecular cell

authors： Loeb GB,Khan AA,Canner D,Hiatt JB,Shendure J,Darnell RB,Leslie CS,Rudensky AY

更新日期：2012-12-14 00:00:00

abstract：:Both H4K16 acetylation and H3K4 trimethylation are required for gene activation. However, it is still largely unclear how these modifications are orchestrated by transcriptional factors. Here, we analyzed the mechanism of the transcriptional activation by FOXP3, an X-linked suppressor of autoimmune diseases and cancer...

journal_title：Molecular cell

authors： Katoh H,Qin ZS,Liu R,Wang L,Li W,Li X,Wu L,Du Z,Lyons R,Liu CG,Liu X,Dou Y,Zheng P,Liu Y

更新日期：2011-12-09 00:00:00