Abstract:
:Homologous recombination (HR) is crucial for genetic exchange and accurate repair of DNA double-strand breaks and is pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly understood. Here, we use time-resolved chromatin immunoprecipitations of repair proteins to monitor homology search in vivo. We found that homology search proceeds by a probing mechanism, which commences around the break and samples preferentially on the broken chromosome. However, elements thought to instruct chromosome loops mediate homology search shortcuts, and centromeres, which cluster within the nucleus, may facilitate homology search on other chromosomes. Our study thus reveals crucial parameters for homology search in vivo and emphasizes the importance of linear distance, chromosome architecture, and proximity for recombination efficiency.
journal_name
Mol Celljournal_title
Molecular cellauthors
Renkawitz J,Lademann CA,Kalocsay M,Jentsch Sdoi
10.1016/j.molcel.2013.02.020subject
Has Abstractpub_date
2013-04-25 00:00:00pages
261-72issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(13)00176-7journal_volume
50pub_type
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