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Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation.

Abstract：

:Networks of protein interactions coordinate cellular functions. We describe a bimolecular fluorescence complementation (BiFC) assay for determination of the locations of protein interactions in living cells. This approach is based on complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are brought together by interactions between proteins fused to each fragment. BiFC analysis was used to investigate interactions among bZIP and Rel family transcription factors. Regions outside the bZIP domains determined the locations of bZIP protein interactions. The subcellular sites of protein interactions were regulated by signaling. Cross-family interactions between bZIP and Rel proteins affected their subcellular localization and modulated transcription activation. These results attest to the general applicability of the BiFC assay for studies of protein interactions.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Hu CD,Chinenov Y,Kerppola TK

doi

10.1016/s1097-2765(02)00496-3

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

789-98

issue

4

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(02)00496-3

journal_volume

9

pub_type

杂志文章

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