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Ring1B compacts chromatin structure and represses gene expression independent of histone ubiquitination.

Abstract：

:How polycomb group proteins repress gene expression in vivo is not known. While histone-modifying activities of the polycomb repressive complexes (PRCs) have been studied extensively, in vitro data have suggested a direct activity of the PRC1 complex in compacting chromatin. Here, we investigate higher-order chromatin compaction of polycomb targets in vivo. We show that PRCs are required to maintain a compact chromatin state at Hox loci in embryonic stem cells (ESCs). There is specific decompaction in the absence of PRC2 or PRC1. This is due to a PRC1-like complex, since decompaction occurs in Ring1B null cells that still have PRC2-mediated H3K27 methylation. Moreover, we show that the ability of Ring1B to restore a compact chromatin state and to repress Hox gene expression is not dependent on its histone ubiquitination activity. We suggest that Ring1B-mediated chromatin compaction acts to directly limit transcription in vivo.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Eskeland R,Leeb M,Grimes GR,Kress C,Boyle S,Sproul D,Gilbert N,Fan Y,Skoultchi AI,Wutz A,Bickmore WA

doi

10.1016/j.molcel.2010.02.032

subject

Has Abstract

pub_date

2010-05-14 00:00:00

pages

452-64

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(10)00249-2

journal_volume

38

pub_type

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