Abstract:
:We have identified human MBT domain-containing protein L3MBTL2 as an integral component of a protein complex that we termed Polycomb repressive complex 1 (PRC1)-like 4 (PRC1L4), given the copresence of PcG proteins RING1, RING2, and PCGF6/MBLR. PRC1L4 also contained E2F6 and CBX3/HP1γ, known to function in transcriptional repression. PRC1L4-mediated repression necessitated L3MBTL2 that compacted chromatin in a histone modification-independent manner. Genome-wide location analyses identified several hundred genes simultaneously bound by L3MBTL2 and E2F6, preferentially around transcriptional start sites that exhibited little overlap with those targeted by other E2Fs or by L3MBTL1, another MBT domain-containing protein that interacts with RB1. L3MBTL2-specific RNAi resulted in increased expression of target genes that exhibited a significant reduction in H2A lysine 119 monoubiquitination. Our findings highlight a PcG/MBT collaboration that attains repressive chromatin without entailing histone lysine methylation marks.
journal_name
Mol Celljournal_title
Molecular cellauthors
Trojer P,Cao AR,Gao Z,Li Y,Zhang J,Xu X,Li G,Losson R,Erdjument-Bromage H,Tempst P,Farnham PJ,Reinberg Ddoi
10.1016/j.molcel.2011.04.004subject
Has Abstractpub_date
2011-05-20 00:00:00pages
438-50issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(11)00258-9journal_volume
42pub_type
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