Abstract：
:Although DNA looping between the initiator binding sites (iterons) of the replication origin (ori) of a plasmid and the iterons located in a cis-acting control sequence called inc has been postulated to promote negative control of plasmid DNA replication, not only was definitive evidence for such looping lacking, but also the detailed molecular mechanism of this control had not been elucidated. Here, we present direct evidence showing that both the monomeric and the dimeric forms of the RepE initiator protein of F factor together promote pairing of incC-oriF sites by DNA looping. By using a reconstituted replication system consisting of 26 purified proteins, we show further that the DNA loop formation negatively regulates plasmid replication by inhibiting the formation of an open complex at the replication origin, thus elucidating a key step of replication control.
journal_name
Mol Celljournal_title
Molecular cellauthors
Zzaman S,Bastia Ddoi
10.1016/j.molcel.2005.10.037subject
Has Abstractpub_date
2005-12-22 00:00:00pages
833-43issue
6eissn
1097-2765issn
1097-4164pii
S1097-2765(05)01732-6journal_volume
20pub_type
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