Abstract：
:SNAP proteins play an essential role in membrane trafficking in eukaryotic cells. They activate and recycle SNARE proteins by serving as adaptors between SNAREs and the cytosolic chaperone NSF. We have determined the crystal structure of Sec17, the yeast homolog of alpha-SNAP, to 2.9 A resolution. Sec17 is composed of an N-terminal twisted sheet of alpha-helical hairpins and a C-terminal alpha-helical bundle. The N-terminal sheet has local similarity to the tetratricopeptide repeats from protein phosphatase 5 but has a different overall twist. Sec17 also shares structural features with HEAT and clathrin heavy chain repeats. Possible models of SNAP:SNARE binding suggest that SNAPs may function as lever arms, transmitting forces generated by conformational changes in NSF/Sec18 to drive disassembly of SNARE complexes.
journal_name
Mol Celljournal_title
Molecular cellauthors
Rice LM,Brunger ATdoi
10.1016/s1097-2765(00)80190-2subject
Has Abstractpub_date
1999-07-01 00:00:00pages
85-95issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80190-2journal_volume
4pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:Homologous recombination (HR) is crucial for genetic exchange and accurate repair of DNA double-strand breaks and is pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly und...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2013.02.020
更新日期：2013-04-25 00:00:00
abstract：:We have identified ARAP1 and ARAP2 and examined ARAP1 as a possible link between phosphoinositide-, Arf-, and Rho-mediated cell signaling. ARAP1 contains Arf GAP, Rho GAP, Ankyrin repeat, Ras-associating, and five PH domains. In vitro, ARAP1 had Rho GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent A...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00428-8
更新日期：2002-01-01 00:00:00
abstract：:In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied ini...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.08.011
更新日期：2016-09-15 00:00:00
abstract：:Both p53 and Mdmx are ubiquitinated and degraded by the same E3 ligase Mdm2; interestingly, however, while p53 is rapidly degraded by Mdm2, Mdmx is a stable protein in most cancer cells. Thus, the mechanism by which Mdmx is degraded by Mdm2 needs further elucidation. Here, we identified the noncoding 5S rRNA as a majo...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.08.020
更新日期：2011-09-16 00:00:00
abstract：:BIK protein is an initiator of mitochondrial apoptosis, and BIK expression is induced by proapoptotic signals, including DNA damage. Here, we demonstrate that 3' end processing and expression of BIK mRNA are controlled by the nuclear PI4,5P(2)-regulated poly(A) polymerase Star-PAP downstream of DNA damage. Nuclear PKC...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.11.017
更新日期：2012-01-13 00:00:00
abstract：:Mitochondrial crista structure partitions vital cellular reactions and is precisely regulated by diverse cellular signals. Here, we show that, in Drosophila, mitochondrial cristae undergo dynamic remodeling among distinct subcellular regions and the Parkinson's disease (PD)-linked Ser/Thr kinase PINK1 participates in ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.01.026
更新日期：2018-03-01 00:00:00
abstract：:TMEM39A, encoding an ER-localized transmembrane protein, is a susceptibility locus for multiple autoimmune diseases. The molecular function of TMEM39A remains completely unknown. Here we demonstrated that TMEM39A, also called SUSR2, modulates autophagy activity by regulating the spatial distribution and levels of PtdI...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.10.035
更新日期：2020-02-06 00:00:00
abstract：:In response to hyperosmotic environments, most eukaryotic cells activate a specialized mitogen-activated protein (MAP) kinase pathway. In S. cerevisiae, the key protein kinase, Hog1, coordinates the transcriptional induction of a variety of genes devoted to osmoadaptation and general stress protection. Depending on th...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(01)00221-0
更新日期：2001-04-01 00:00:00
abstract：:Myosin VI is the only myosin that moves toward the minus end of actin filaments, suggesting a unique biological function. Here, we show that myosin VI is present in the nucleus of mammalian cells where it colocalizes with newly transcribed mRNA and with RNA polymerase II (RNAPII) and is detected in the RNAPII complex....
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.07.005
更新日期：2006-09-01 00:00:00
abstract：:Fluoxetine (Prozac) is an antidepressant that is thought to act by blocking presynaptic reuptake of the neurotransmitter serotonin. Despite widespread clinical use of fluoxetine, direct evidence for this mechanism has been difficult to obtain in vivo. We have determined that fluoxetine has an additional neuromuscular ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80362-7
更新日期：1999-08-01 00:00:00
abstract：:Triplex structure-forming GAA/TTC repeats pose a dual threat to the eukaryotic genome integrity. Their potential to expand can lead to gene inactivation, the cause of Friedreich's ataxia disease in humans. In model systems, long GAA/TTC tracts also act as chromosomal fragile sites that can trigger gross chromosomal re...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.08.002
更新日期：2012-10-26 00:00:00
abstract：:In Parkinson's disease (PD), α-synuclein (αS) pathologically impacts the brain, a highly lipid-rich organ. We investigated how alterations in αS or lipid/fatty acid homeostasis affect each other. Lipidomic profiling of human αS-expressing yeast revealed increases in oleic acid (OA, 18:1), diglycerides, and triglycerid...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.11.028
更新日期：2019-03-07 00:00:00
abstract：:Certain nascent peptide sequences, when within the ribosomal exit tunnel, can inhibit translation termination and/or peptide elongation. The 24 residue leader peptidyl-tRNA of the tna operon of E. coli, TnaC-tRNA(Pro), in the presence of excess tryptophan, resists cleavage at the tnaC stop codon. TnaC residue Trp12 is...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.06.013
更新日期：2005-08-05 00:00:00
abstract：:The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, an...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80066-0
更新日期：1998-04-01 00:00:00
abstract：:Heterochromatin is required to restrict aberrant expression of retrotransposons, but it remains poorly defined due to the underlying repeat-rich sequences. We dissected Suv39h-dependent histone H3 lysine 9 trimethylation (H3K9me3) by genome-wide ChIP sequencing in mouse embryonic stem cells (ESCs). Refined bioinformat...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2014.05.029
更新日期：2014-07-17 00:00:00
abstract：:Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.02.011
更新日期：2009-03-27 00:00:00
abstract：:We used computational algorithms to find conserved sequences in the 3' untranslated region (UTR) of transcripts that exhibited rapid decay in primary human T cells and found that the consensus sequence UGUUUGUUUGU, which we have termed a GU-rich element (GRE), was enriched in short-lived transcripts. Using a tet-off r...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.11.024
更新日期：2008-02-01 00:00:00
abstract：:Comparisons of bacteriophage PRD1 and adenovirus protein structures and virion architectures have been instrumental in unraveling an evolutionary relationship and have led to a proposal of a phylogeny-based virus classification. The structure of the PRD1 spike protein P5 provides further insight into the evolution of ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.03.019
更新日期：2005-04-15 00:00:00
abstract：:Vascular endothelial (VE)-cadherin homophilic adhesion controls endothelial barrier permeability through assembly of adherens junctions (AJs). We observed that loss of VE-cadherin-mediated adhesion induced the activation of Src and phospholipase C (PLC)γ2, which mediated Ca(2+) release from endoplasmic reticulum (ER) ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.10.011
更新日期：2012-12-28 00:00:00
abstract：:The redundancy of the genetic code implies that most amino acids are encoded by multiple synonymous codons. In all domains of life, a biased frequency of synonymous codons is observed at the genome level, in functionally related genes (e.g., in operons), and within single genes. Other codon bias variants include biase...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2015.05.035
更新日期：2015-07-16 00:00:00
abstract：:SPT5 and its binding partner SPT4 function in both positively and negatively regulating transcriptional elongation. The demonstration that SPT5 and RNA polymerase II are targets for phosphorylation by CDK9/cyclin T1 indicates that posttranslational modifications of these factors are important in regulating the elongat...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00101-1
更新日期：2003-04-01 00:00:00
abstract：:The binding of cytoplasmic proteins, such as talin, to the cytoplasmic domains of integrin adhesion receptors mediates bidirectional signal transduction. Here we report the crystal structure of the principal integrin binding and activating fragment of talin, alone and in complex with fragments of the beta 3 integrin t...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00823-7
更新日期：2003-01-01 00:00:00
abstract：:Corticotropin-releasing factor (CRF) and the three related peptides urocortins 1-3 (UCN1-UCN3) are endocrine hormones that control the stress responses by activating CRF1R and CRF2R, two members of class B G-protein-coupled receptors (GPCRs). Here, we present two cryoelectron microscopy (cryo-EM) structures of UCN1-bo...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.01.013
更新日期：2020-02-06 00:00:00
abstract：:Oncogene-induced senescence (OIS) is a tumor-suppressive mechanism typified by stable proliferative arrest, a persistent DNA damage response, and the senescence-associated secretory phenotype (SASP), which helps to maintain the senescent state and triggers bystander senescence in a paracrine fashion. Here, we demonstr...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2015.07.011
更新日期：2015-09-03 00:00:00
abstract：:Ubiquitin (UB)-driven signaling systems permeate biology, and are often integrated with other types of post-translational modifications (PTMs), including phosphorylation. Flux through such pathways is dictated by the fractional stoichiometry of distinct modifications and protein assemblies as well as the spatial organ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2015.02.020
更新日期：2015-05-21 00:00:00
abstract：:Cryo-EM structures of Dot1L in complex with a ubiquitinated nucleosome provide the long-sought-after molecular mechanism of Dot1L-mediated methylation of lysine 79 in histone H3 and explain crosstalk with histone H2B ubiquitination. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2019.05.013
更新日期：2019-06-06 00:00:00
abstract：:In order to determine the global effects of oncogenic Ras and Akt signaling pathways on translational efficiencies, we compared the gene expression profiles of total cellular mRNA and mRNA associated with polysomes. We found that the immediate effect of Ras and Akt signaling blockade on transcription was relatively mo...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00395-2
更新日期：2003-10-01 00:00:00
abstract：:JunD is the most broadly expressed member of the Jun family and the AP-1 transcription factor complex. Primary fibroblasts lacking JunD displayed p53-dependent growth arrest, upregulated p19(Arf) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)00109-x
更新日期：2000-11-01 00:00:00
abstract：:The signal transducers of the transforming growth factor beta (TGFbeta)/bone morphogenetic protein (BMP), the Smads, promote the expression of a subset of miRNAs by facilitating the cleavage reaction by Drosha. The mechanism that limits Smad-mediated processing to a selective group of miRNAs remained hitherto unexplor...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.07.011
更新日期：2010-08-13 00:00:00
abstract：:Allosteric communication between interacting molecules is fundamental to signal transduction and many other cellular processes. To better understand the relationship between nuclear receptor (NR) ligand positioning and the formation of the coactivator binding pocket, we investigated the determinants of ligand selectiv...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(04)00054-1
更新日期：2004-02-13 00:00:00
