Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha.

Abstract:

:The signal transducers of the transforming growth factor beta (TGFbeta)/bone morphogenetic protein (BMP), the Smads, promote the expression of a subset of miRNAs by facilitating the cleavage reaction by Drosha. The mechanism that limits Smad-mediated processing to a selective group of miRNAs remained hitherto unexplored. In this study, we expand the number of TGFbeta/BMP-regulated miRNAs (T/B-miRs) to 20. Of interest, a majority of T/B-miRs contain a consensus sequence (R-SBE) within the stem region of the primary transcripts of T/B-miRs (pri-T/B-miRs). Here, we demonstrate that Smads directly bind the R-SBE. Mutation of the R-SBE abrogates TGFbeta/BMP-induced recruitment of Smads, Drosha, and DGCR8 to pri-T/B-miRs and impairs their processing, whereas introduction of R-SBE to unregulated pri-miRNAs is sufficient to recruit Smads and to allow regulation by TGFbeta/BMP. Thus, Smads are multifunctional proteins that modulate gene expression transcriptionally through DNA binding and posttranscriptionally through pri-miRNA binding and regulation of miRNA processing.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Davis BN,Hilyard AC,Nguyen PH,Lagna G,Hata A

doi

10.1016/j.molcel.2010.07.011

subject

Has Abstract

pub_date

2010-08-13 00:00:00

pages

373-84

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(10)00533-2

journal_volume

39

pub_type

杂志文章
  • Phosphatidic Acid Signals via the Hippo Pathway.

    abstract::In this issue of Molecular Cell, Han et al. (2018) show that phosphatidic acid inhibits the action of the LATS kinases, key mediators of Hippo pathway signaling, which leads to an increase in the nuclear activity of the transcriptional regulator YAP. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2018.10.004

    authors: Stampouloglou E,Varelas X

    更新日期:2018-10-18 00:00:00

  • Summon SUMO to wrestle with inflammation.

    abstract::In this issue of Molecular Cell, Lee et al. (2009) report that SUMOylation of nuclear receptors LXRalpha and LXRbeta plays a critical role in the transrepression of IFN-gamma-induced STAT1-dependent inflammatory responses in brain astrocytes. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2009.09.002

    authors: Liu B,Shuai K

    更新日期:2009-09-24 00:00:00

  • New functions for Y family polymerases.

    abstract::The Y family of DNA polymerases plays crucial roles in carrying out translesion synthesis past damaged bases in DNA. Several recent papers suggest that they might have other roles as well in gene conversion, in nucleotide excision repair (NER), and in DNA replication under stressed conditions. ...

    journal_title:Molecular cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.molcel.2006.10.021

    authors: Lehmann AR

    更新日期:2006-11-17 00:00:00

  • Mitochondrial and nuclear accumulation of the transcription factor ATFS-1 promotes OXPHOS recovery during the UPR(mt).

    abstract::Mitochondrial diseases and aging are associated with defects in the oxidative phosphorylation machinery (OXPHOS), which are the only complexes composed of proteins encoded by separate genomes. To better understand genome coordination and OXPHOS recovery during mitochondrial dysfunction, we examined ATFS-1, a transcrip...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2015.02.008

    authors: Nargund AM,Fiorese CJ,Pellegrino MW,Deng P,Haynes CM

    更新日期:2015-04-02 00:00:00

  • The tangled circuitry of metabolism and apoptosis.

    abstract::For single-cell organisms, nutrient uptake and metabolism are central to the fundamental decision of whether to grow or divide. In metazoans, cell fate decisions are more complex: organismal homeostasis must be strictly maintained by balancing cell proliferation and death. Despite this increased complexity, cell fate ...

    journal_title:Molecular cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.molcel.2012.12.026

    authors: Andersen JL,Kornbluth S

    更新日期:2013-02-07 00:00:00

  • Dynamics of substrate denaturation and translocation by the ClpXP degradation machine.

    abstract::ClpXP is a protein machine composed of the ClpX ATPase, a member of the Clp/Hsp100 family of remodeling enzymes, and the ClpP peptidase. Here, ClpX and ClpXP are shown to catalyze denaturation of GFP modified with an ssrA degradation tag. ClpX translocates this denatured protein into the proteolytic chamber of ClpP an...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80243-9

    authors: Kim YI,Burton RE,Burton BM,Sauer RT,Baker TA

    更新日期:2000-04-01 00:00:00

  • Arginine methylation modulates autophagic degradation of PGL granules in C. elegans.

    abstract::The selective degradation of intracellular components by autophagy involves sequential interactions of the cargo with a receptor, which also binds the autophagosomal protein Atg8 and a scaffold protein. Here, we demonstrated that mutations in C. elegans epg-11, which encodes an arginine methyltransferase homologous to...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2013.09.014

    authors: Li S,Yang P,Tian E,Zhang H

    更新日期:2013-11-07 00:00:00

  • Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O.

    abstract::The tumor suppressor BAP1 interacts with chromatin-associated proteins and regulates cell proliferation, but its mechanism of action and regulation remain poorly defined. We show that the ubiquitin-conjugating enzyme UBE2O multi-monoubiquitinates the nuclear localization signal of BAP1, thereby inducing its cytoplasmi...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2014.03.002

    authors: Mashtalir N,Daou S,Barbour H,Sen NN,Gagnon J,Hammond-Martel I,Dar HH,Therrien M,Affar el B

    更新日期:2014-05-08 00:00:00

  • Compartmentalization of the cell cortex by septins is required for maintenance of cell polarity in yeast.

    abstract::Formation and maintenance of specialized plasma membrane domains are crucial for many biological processes, such as cell polarization and signaling. During isotropic bud growth, the yeast cell periphery is divided into two domains: the bud surface, an active site of exocytosis and growth, and the relatively quiescent ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80324-x

    authors: Barral Y,Mermall V,Mooseker MS,Snyder M

    更新日期:2000-05-01 00:00:00

  • ATXN7L3 and ENY2 Coordinate Activity of Multiple H2B Deubiquitinases Important for Cellular Proliferation and Tumor Growth.

    abstract::Histone H2B monoubiquitination (H2Bub1) is centrally involved in gene regulation. The deubiquitination module (DUBm) of the SAGA complex is a major regulator of global H2Bub1 levels, and components of this DUBm are linked to both neurodegenerative diseases and cancer. Unexpectedly, we find that ablation of USP22, the ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2016.03.030

    authors: Atanassov BS,Mohan RD,Lan X,Kuang X,Lu Y,Lin K,McIvor E,Li W,Zhang Y,Florens L,Byrum SD,Mackintosh SG,Calhoun-Davis T,Koutelou E,Wang L,Tang DG,Tackett AJ,Washburn MP,Workman JL,Dent SY

    更新日期:2016-05-19 00:00:00

  • Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.

    abstract::The C-terminal fragment, Bb, of factor B combines with C3b to form the pivotal C3-convertase, C3bBb, of alternative complement pathway. Bb consists of a von Willebrand factor type A (vWFA) domain that is structurally similar to the I domains of integrins and a serine protease (SP) domain that is in inactive conformati...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(04)00160-1

    authors: Ponnuraj K,Xu Y,Macon K,Moore D,Volanakis JE,Narayana SV

    更新日期:2004-04-09 00:00:00

  • Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING.

    abstract::The presence of microbial or self DNA in the cytoplasm of mammalian cells is a danger signal detected by the DNA sensor cyclic-GMP-AMP (cGAMP) synthase (cGAS), which catalyzes the production of cGAMP that in turn serves as a second messenger to activate innate immune responses. Here we show that endogenous cGAMP in ma...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2013.05.022

    authors: Zhang X,Shi H,Wu J,Zhang X,Sun L,Chen C,Chen ZJ

    更新日期:2013-07-25 00:00:00

  • Global Mapping of Human RNA-RNA Interactions.

    abstract::The majority of the human genome is transcribed into non-coding (nc)RNAs that lack known biological functions or else are only partially characterized. Numerous characterized ncRNAs function via base pairing with target RNA sequences to direct their biological activities, which include critical roles in RNA processing...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2016.04.030

    authors: Sharma E,Sterne-Weiler T,O'Hanlon D,Blencowe BJ

    更新日期:2016-05-19 00:00:00

  • Missing the Mark: PRDM9-Dependent Methylation Is Required for Meiotic DSB Targeting.

    abstract::PRDM9 determines the localization of meiotic recombination hotspots, which are associated with histone H3 methylation. It is not known whether PRDM9's methyltransferase activity is required or how some PRDM9 alleles can dominate the distribution of hotspots over other alleles. Diagouraga, Clément, and colleagues (2018...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2018.02.021

    authors: Kang R,Zelazowski MJ,Cole F

    更新日期:2018-03-01 00:00:00

  • CREB binding protein recruitment to the transcription complex requires growth factor-dependent phosphorylation of its GF box.

    abstract::Growth factors such as epidermal growth factor (EGF) and insulin regulate development and metabolism via genes containing both POU homeodomain (Pit-1) and phorbol ester (AP-1) response elements. Although CREB binding protein (CBP) functions as a coactivator on these elements, the mechanism of transactivation was previ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(01)00202-7

    authors: Zanger K,Radovick S,Wondisford FE

    更新日期:2001-03-01 00:00:00

  • A Eukaryotic Sensor for Membrane Lipid Saturation.

    abstract::Maintaining a fluid bilayer is essential for cell signaling and survival. Lipid saturation is a key factor determining lipid packing and membrane fluidity, and it must be tightly controlled to guarantee organelle function and identity. A dedicated eukaryotic mechanism of lipid saturation sensing, however, remains elus...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2016.05.015

    authors: Covino R,Ballweg S,Stordeur C,Michaelis JB,Puth K,Wernig F,Bahrami A,Ernst AM,Hummer G,Ernst R

    更新日期:2016-07-07 00:00:00

  • Linkage-specific avidity defines the lysine 63-linked polyubiquitin-binding preference of rap80.

    abstract::Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2009.02.011

    authors: Sims JJ,Cohen RE

    更新日期:2009-03-27 00:00:00

  • Interplay of TBP inhibitors in global transcriptional control.

    abstract::The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Here, we use DNA microarrays to explore the genome-wide interplay of several TBP-interacting inhibitors in the yeast Saccharomyces cerevisiae. Our findings suggest the following: Th...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(02)00683-4

    authors: Chitikila C,Huisinga KL,Irvin JD,Basehoar AD,Pugh BF

    更新日期:2002-10-01 00:00:00

  • ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks.

    abstract::Double-strand breaks (DSBs) of DNA in eukaryotic cells are predominantly repaired by non-homologous end joining (NHEJ). The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2017.08.021

    authors: Lee KY,Im JS,Shibata E,Dutta A

    更新日期:2017-10-05 00:00:00

  • The MRN-CtIP pathway is required for metaphase chromosome alignment.

    abstract::Faithful duplication of the genome in S phase followed by its accurate segregation in mitosis is essential to maintain genomic integrity. Recent studies have suggested that proteins involved in DNA transactions are also required for whole-chromosome stability. Here we demonstrate that the MRN (Mre11, Rad50, and Nbs1) ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2013.01.023

    authors: Rozier L,Guo Y,Peterson S,Sato M,Baer R,Gautier J,Mao Y

    更新日期:2013-03-28 00:00:00

  • Ribonucleotide Reductase Requires Subunit Switching in Hypoxia to Maintain DNA Replication.

    abstract::Cells exposed to hypoxia experience replication stress but do not accumulate DNA damage, suggesting sustained DNA replication. Ribonucleotide reductase (RNR) is the only enzyme capable of de novo synthesis of deoxyribonucleotide triphosphates (dNTPs). However, oxygen is an essential cofactor for mammalian RNR (RRM1/RR...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2017.03.005

    authors: Foskolou IP,Jorgensen C,Leszczynska KB,Olcina MM,Tarhonskaya H,Haisma B,D'Angiolella V,Myers WK,Domene C,Flashman E,Hammond EM

    更新日期:2017-04-20 00:00:00

  • Odilorhabdins, Antibacterial Agents that Cause Miscoding by Binding at a New Ribosomal Site.

    abstract::Growing resistance of pathogenic bacteria and shortage of antibiotic discovery platforms challenge the use of antibiotics in the clinic. This threat calls for exploration of unconventional sources of antibiotics and identification of inhibitors able to eradicate resistant bacteria. Here we describe a different class o...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2018.03.001

    authors: Pantel L,Florin T,Dobosz-Bartoszek M,Racine E,Sarciaux M,Serri M,Houard J,Campagne JM,de Figueiredo RM,Midrier C,Gaudriault S,Givaudan A,Lanois A,Forst S,Aumelas A,Cotteaux-Lautard C,Bolla JM,Vingsbo Lundberg C,Huseby

    更新日期:2018-04-05 00:00:00

  • Non-catalytic Roles for XPG with BRCA1 and BRCA2 in Homologous Recombination and Genome Stability.

    abstract::XPG is a structure-specific endonuclease required for nucleotide excision repair, and incision-defective XPG mutations cause the skin cancer-prone syndrome xeroderma pigmentosum. Truncating mutations instead cause the neurodevelopmental progeroid disorder Cockayne syndrome, but little is known about how XPG loss resul...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2015.12.026

    authors: Trego KS,Groesser T,Davalos AR,Parplys AC,Zhao W,Nelson MR,Hlaing A,Shih B,Rydberg B,Pluth JM,Tsai MS,Hoeijmakers JHJ,Sung P,Wiese C,Campisi J,Cooper PK

    更新日期:2016-02-18 00:00:00

  • Autoinhibition of Bcr-Abl through its SH3 domain.

    abstract::Bcr-Abl is a dysregulated tyrosine kinase whose mechanism of activation is unclear. Here, we demonstrate that, like c-Abl, Bcr-Abl is negatively regulated through its SH3 domain. Kinase activity, transformation, and leukemogenesis by Bcr-Abl are greatly impaired by mutations of the Bcr coiled-coil domain that disrupt ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(03)00274-0

    authors: Smith KM,Yacobi R,Van Etten RA

    更新日期:2003-07-01 00:00:00

  • HDAC6 modulates cell motility by altering the acetylation level of cortactin.

    abstract::Histone deacetylase 6 (HDAC6) is a tubulin-specific deacetylase that regulates microtubule-dependent cell movement. In this study, we identify the F-actin-binding protein cortactin as a HDAC6 substrate. We demonstrate that HDAC6 binds cortactin and that overexpression of HDAC6 leads to hypoacetylation of cortactin, wh...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2007.05.033

    authors: Zhang X,Yuan Z,Zhang Y,Yong S,Salas-Burgos A,Koomen J,Olashaw N,Parsons JT,Yang XJ,Dent SR,Yao TP,Lane WS,Seto E

    更新日期:2007-07-20 00:00:00

  • Activation of the bacterial sensor kinase PhoQ by acidic pH.

    abstract::The Salmonellae PhoQ sensor kinase senses the mammalian phagosome environment to activate a transcriptional program essential for virulence. The PhoQ periplasmic domain binds divalent cations, forming bridges with inner membrane phospholipids to maintain PhoQ repression. PhoQ also binds and is activated by cationic an...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2007.03.008

    authors: Prost LR,Daley ME,Le Sage V,Bader MW,Le Moual H,Klevit RE,Miller SI

    更新日期:2007-04-27 00:00:00

  • An mTORC1-Mdm2-Drosha axis for miRNA biogenesis in response to glucose- and amino acid-deprivation.

    abstract::mTOR senses nutrient and energy status to regulate cell survival and metabolism in response to environmental changes. Surprisingly, targeted mutation of Tsc1, a negative regulator of mTORC1, caused a broad reduction in miRNAs due to Drosha degradation. Conversely, targeted mutation of Raptor, an essential component of...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2014.12.034

    authors: Ye P,Liu Y,Chen C,Tang F,Wu Q,Wang X,Liu CG,Liu X,Liu R,Liu Y,Zheng P

    更新日期:2015-02-19 00:00:00

  • Herpes simplex virus glycoprotein D bound to the human receptor HveA.

    abstract::Herpes simplex virus (HSV) infection requires binding of the viral envelope glycoprotein D (gD) to cell surface receptors. We report the X-ray structures of a soluble, truncated ectodomain of gD both alone and in complex with the ectodomain of its cellular receptor HveA. Two bound anions suggest possible binding sites...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(01)00298-2

    authors: Carfí A,Willis SH,Whitbeck JC,Krummenacher C,Cohen GH,Eisenberg RJ,Wiley DC

    更新日期:2001-07-01 00:00:00

  • Mitotic wnt signaling promotes protein stabilization and regulates cell size.

    abstract::Canonical Wnt signaling is thought to regulate cell behavior mainly by inducing β-catenin-dependent transcription of target genes. In proliferating cells Wnt signaling peaks in the G2/M phase of the cell cycle, but the significance of this "mitotic Wnt signaling" is unclear. Here we introduce Wnt-dependent stabilizati...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2014.04.014

    authors: Acebron SP,Karaulanov E,Berger BS,Huang YL,Niehrs C

    更新日期:2014-05-22 00:00:00

  • Activation of a primed RING E3-E2-ubiquitin complex by non-covalent ubiquitin.

    abstract::RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2015.02.017

    authors: Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

    更新日期:2015-04-16 00:00:00