Abstract：
:Sister-chromatid cohesion describes the orderly association of newly replicated DNA molecules behind replication forks. It plays an essential role in the maintenance and faithful transmission of genetic information. Cohesion is created by DNA topological links and proteinaceous bridges, whose formation and deposition could be potentially affected by many processes. Current knowledge on cohesion has been mainly gained by fluorescence microscopy observation. However, the resolution limit of microscopy and the restricted number of genomic positions that can be simultaneously visualized considerably hampered progress. Here, we present a high-throughput methodology to monitor sister-chromatid contacts (Hi-SC2). Using the multi-chromosomal Vibrio cholerae bacterium as a model, we show that Hi-SC2 permits to monitor local variations in sister-chromatid cohesion at a high resolution over a whole genome.
journal_name
Mol Celljournal_title
Molecular cellauthors
Espinosa E,Paly E,Barre FXdoi
10.1016/j.molcel.2020.06.033subject
Has Abstractpub_date
2020-09-03 00:00:00pages
857-869.e3issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(20)30435-4journal_volume
79pub_type
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