Abstract:
:p300/CBP and PCAF coactivators have acetyltransferase activities and regulate transcription, cell cycle progression, and differentiation. They are both required for MyoD activity and muscle differentiation. Nevertheless, their roles must be different since the acetyltransferase activity of PCAF but not of p300 is involved in controlling myogenic transcription and differentiation. Here, we provide a molecular explanation of this phenomenon and report that MyoD is directly acetylated by PCAF at evolutionarily conserved lysines. Acetylated MyoD displays an increased affinity for its DNA target. Importantly, conservative substitutions of acetylated lysines with nonacetylatable arginines impair the ability of MyoD to stimulate transcription and to induce muscle conversion indicating that acetylation of MyoD is functionally critical.
journal_name
Mol Celljournal_title
Molecular cellauthors
Sartorelli V,Puri PL,Hamamori Y,Ogryzko V,Chung G,Nakatani Y,Wang JY,Kedes Ldoi
10.1016/s1097-2765(00)80383-4subject
Has Abstractpub_date
1999-11-01 00:00:00pages
725-34issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80383-4journal_volume
4pub_type
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