Acetylation of MyoD directed by PCAF is necessary for the execution of the muscle program.

Abstract:

:p300/CBP and PCAF coactivators have acetyltransferase activities and regulate transcription, cell cycle progression, and differentiation. They are both required for MyoD activity and muscle differentiation. Nevertheless, their roles must be different since the acetyltransferase activity of PCAF but not of p300 is involved in controlling myogenic transcription and differentiation. Here, we provide a molecular explanation of this phenomenon and report that MyoD is directly acetylated by PCAF at evolutionarily conserved lysines. Acetylated MyoD displays an increased affinity for its DNA target. Importantly, conservative substitutions of acetylated lysines with nonacetylatable arginines impair the ability of MyoD to stimulate transcription and to induce muscle conversion indicating that acetylation of MyoD is functionally critical.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Sartorelli V,Puri PL,Hamamori Y,Ogryzko V,Chung G,Nakatani Y,Wang JY,Kedes L

doi

10.1016/s1097-2765(00)80383-4

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

725-34

issue

5

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(00)80383-4

journal_volume

4

pub_type

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