A robust toolkit for functional profiling of the yeast genome.

abstract：:Dysregulation of Wnt signaling has been implicated in tumorigenesis. The role of Transducin β-like proteins TBL1-TBLR1 in the promotion of Wnt/β-catenin-mediated oncogenesis has recently been emphasized; however, the molecular basis of activation of Wnt signaling by the corepressor TBL1-TBLR1 is incompletely understoo...

journal_title：Molecular cell

authors： Choi HK,Choi KC,Yoo JY,Song M,Ko SJ,Kim CH,Ahn JH,Chun KH,Yook JI,Yoon HG

更新日期：2011-07-22 00:00:00

abstract：:Upon heterologous overexpression, many proteins misfold or aggregate, thus resulting in low functional yields. Human acetylcholinesterase (hAChE), an enzyme mediating synaptic transmission, is a typical case of a human protein that necessitates mammalian systems to obtain functional expression. We developed a computat...

journal_title：Molecular cell

authors： Goldenzweig A,Goldsmith M,Hill SE,Gertman O,Laurino P,Ashani Y,Dym O,Unger T,Albeck S,Prilusky J,Lieberman RL,Aharoni A,Silman I,Sussman JL,Tawfik DS,Fleishman SJ

更新日期：2016-07-21 00:00:00

abstract：:Cells undergo apoptosis upon exposure to severe DNA damage stress. Under this condition, p73 is phosphorylated and activated by c-Abl. The transcription coactivator Yap1 binds p73 to generate a complex that escapes p73 proteasomal degradation and recruits p300 to support transcription of proapoptotic genes. However, t...

journal_title：Molecular cell

authors： Levy D,Adamovich Y,Reuven N,Shaul Y

更新日期：2008-02-15 00:00:00

abstract：:We have used gene competition to distinguish between possible mechanisms of transcriptional activation of the genes of the human beta-globin locus. The insertion of a second beta-globin gene at different points in the locus shows that the more proximal beta gene competes more effectively for activation by the locus co...

journal_title：Molecular cell

authors： Dillon N,Trimborn T,Strouboulis J,Fraser P,Grosveld F

更新日期：1997-12-01 00:00:00

abstract：:Prions constitute a rare class of protein, which can switch to a robust amyloid form and then propagate that form in the absence of a nucleic acid determinant, thereby creating a unique, protein-only infectious agent. Details of the mechanism that drives conversion to the prion form and then subsequent propagation of ...

journal_title：Molecular cell

authors： Tuite MF,Koloteva-Levin N

更新日期：2004-06-04 00:00:00

abstract：:Defective ER-resident membrane proteins need to be ejected into the cytoplasm in order to be degraded by the proteasome, but the exact mechanism remains unclear. In this issue of Molecular Cell, Neal et al. (2018) reveal that the rhomboid pseudoprotease Dfm1 defines the central ERAD component for membrane protein disl...

journal_title：Molecular cell

authors： Avci D,Lemberg MK

更新日期：2018-01-18 00:00:00

abstract：:The recruitment of the TATA box-binding protein (TBP) to promoters in vivo is often rate limiting in gene expression. We present evidence that TBP negatively autoregulates its accessibility to promoter DNA in yeast through dimerization. The crystal structure of TBP dimers was used to design point mutations in the dime...

journal_title：Molecular cell

authors： Jackson-Fisher AJ,Chitikila C,Mitra M,Pugh BF

更新日期：1999-06-01 00:00:00

abstract：:HIV-1 Vpu interacts with CD4 in the endoplasmic reticulum and triggers CD4 degradation, presumably by proteasomes. Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary complexes have been detected by coimmunoprecipitation. beta TrCP binding to Vpu...

journal_title：Molecular cell

authors： Margottin F,Bour SP,Durand H,Selig L,Benichou S,Richard V,Thomas D,Strebel K,Benarous R

更新日期：1998-03-01 00:00:00

abstract：:Growing resistance of pathogenic bacteria and shortage of antibiotic discovery platforms challenge the use of antibiotics in the clinic. This threat calls for exploration of unconventional sources of antibiotics and identification of inhibitors able to eradicate resistant bacteria. Here we describe a different class o...

journal_title：Molecular cell

authors： Pantel L,Florin T,Dobosz-Bartoszek M,Racine E,Sarciaux M,Serri M,Houard J,Campagne JM,de Figueiredo RM,Midrier C,Gaudriault S,Givaudan A,Lanois A,Forst S,Aumelas A,Cotteaux-Lautard C,Bolla JM,Vingsbo Lundberg C,Huseby

更新日期：2018-04-05 00:00:00

abstract：:The WTX gene is frequently lost or mutated in Wilms tumor. In this issue of Molecular Cell, Kim et al. (2012) identify WTX modulation of p53 tumor-suppressor activity through regulation of p53 acetylation. Therefore, WTX differentially regulates the oncogenic β-catenin pathway and the tumor-suppressing p53 pathway. ...

journal_title：Molecular cell

authors： Dai C,Gu W

更新日期：2012-03-09 00:00:00

abstract：:Mitochondrial diseases and aging are associated with defects in the oxidative phosphorylation machinery (OXPHOS), which are the only complexes composed of proteins encoded by separate genomes. To better understand genome coordination and OXPHOS recovery during mitochondrial dysfunction, we examined ATFS-1, a transcrip...

journal_title：Molecular cell

authors： Nargund AM,Fiorese CJ,Pellegrino MW,Deng P,Haynes CM

更新日期：2015-04-02 00:00:00

abstract：:Among the five KCNQ channels, also known as the Kv7 voltage-gated potassium (Kv) channels, KCNQ2-KCNQ5 control neuronal excitability. Dysfunctions of KCNQ2-KCNQ5 are associated with neurological disorders such as epilepsy, deafness, and neuropathic pain. Here, we report the cryoelectron microscopy (cryo-EM) structures...

journal_title：Molecular cell

authors： Li T,Wu K,Yue Z,Wang Y,Zhang F,Shen H

更新日期：2021-01-07 00:00:00

abstract：:Cardiac disease remains the leading cause of morbidity and mortality worldwide. The β1-adrenergic receptor (β1-AR) is a major regulator of cardiac functions and is downregulated in the majority of heart failure cases. A key physiological process is the activation of heterotrimeric G-protein Gs by β1-ARs, leading to in...

journal_title：Molecular cell

authors： Su M,Zhu L,Zhang Y,Paknejad N,Dey R,Huang J,Lee MY,Williams D,Jordan KD,Eng ET,Ernst OP,Meyerson JR,Hite RK,Walz T,Liu W,Huang XY

更新日期：2020-10-01 00:00:00

abstract：:Metazoan transcription factors typically regulate large numbers of genes. Here we identify via a CRISPR-Cas9 genetic screen ZNF410, a pentadactyl DNA-binding protein that in human erythroid cells directly activates only a single gene, the NuRD component CHD4. Specificity is conveyed by two highly evolutionarily conser...

journal_title：Molecular cell

authors： Lan X,Ren R,Feng R,Ly LC,Lan Y,Zhang Z,Aboreden N,Qin K,Horton JR,Grevet JD,Mayuranathan T,Abdulmalik O,Keller CA,Giardine B,Hardison RC,Crossley M,Weiss MJ,Cheng X,Shi J,Blobel GA

更新日期：2021-01-21 00:00:00

abstract：:We have developed a highly parallel strategy, systematic gene-to-phenotype arrays (SGPAs), to comprehensively map the genetic landscape driving molecular phenotypes of interest. By this approach, a complete yeast genetic mutant array is crossed with fluorescent reporters and imaged on membranes at high density and con...

journal_title：Molecular cell

authors： Jaeger PA,Ornelas L,McElfresh C,Wong LR,Hampton RY,Ideker T

更新日期：2018-01-18 00:00:00

abstract：:X-linked mental retardation (XLMR) is an inherited disorder that mostly affects males and is caused by mutations in genes located on the X chromosome. Here, we show that the XLMR protein PHF8 and a C. elegans homolog F29B9.2 catalyze demethylation of di- and monomethylated lysine 9 of histone H3 (H3K9me2/me1). The PHD...

journal_title：Molecular cell

authors： Kleine-Kohlbrecher D,Christensen J,Vandamme J,Abarrategui I,Bak M,Tommerup N,Shi X,Gozani O,Rappsilber J,Salcini AE,Helin K

更新日期：2010-04-23 00:00:00

abstract：:Errors in translation cause cytotoxic protein misfolding and aggregation. In this issue, Ling et al. (2012) show that scavenging or suppression of reactive oxygen species in E. coli reduces the cellular costs of error-induced aggregation. ...

journal_title：Molecular cell

authors： Drummond DA

更新日期：2012-12-14 00:00:00

abstract：:Two new studies provide in vivo ( [this issue of Molecular Cell]) and in vitro ( [this issue of Molecular Cell]) evidence that poleta functions to extend 3' strands exchanged during homologous recombination and raise the issue of how TLS polymerases are selected onto different substrates. ...

journal_title：Molecular cell

authors： Rattray AJ,Strathern JN

更新日期：2005-12-09 00:00:00

abstract：:Cas4 nucleases are conserved factors in many CRISPR systems, yet their molecular role has remained enigmatic. In this issue of Molecular Cell, Shiimori et al. (2018) report that two Cas4 nucleases together determine the size, orientation, and PAM for foreign DNA snippets acquired by CRISPR loci as immunological memory...

journal_title：Molecular cell

authors： Hou Z,Zhang Y

更新日期：2018-06-07 00:00:00

abstract：:The Bloom syndrome helicase BLM and topoisomerase-IIβ-binding protein 1 (TopBP1) are key regulators of genome stability. It was recently proposed that BLM phosphorylation on Ser338 mediates its interaction with TopBP1, to protect BLM from ubiquitylation and degradation (Wang et al., 2013). Here, we show that the BLM-T...

journal_title：Molecular cell

authors： Blackford AN,Nieminuszczy J,Schwab RA,Galanty Y,Jackson SP,Niedzwiedz W

更新日期：2015-03-19 00:00:00

abstract：:Siz1 is a founding member of the Siz/PIAS RING family of SUMO E3 ligases. The X-ray structure of an active Siz1 ligase revealed an elongated tripartite architecture comprised of an N-terminal PINIT domain, a central zinc-containing RING-like SP-RING domain, and a C-terminal domain we term the SP-CTD. Structure-based m...

journal_title：Molecular cell

authors： Yunus AA,Lima CD

更新日期：2009-09-11 00:00:00

abstract：:CREB-binding protein (CBP) possesses an intrinsic acetyltransferase activity capable of acetylating nucleosomal histones as well as several nonhistone proteins. Here, it is shown that CBP can acetylate hepatocyte nuclear factor-4 (HNF-4), a member of the nuclear hormone receptor family, at lysine residues within the n...

journal_title：Molecular cell

authors： Soutoglou E,Katrakili N,Talianidis I

更新日期：2000-04-01 00:00:00

abstract：:Circadian rhythms in mammals are generated by a transcriptional negative feedback loop that is driven primarily by oscillations of PER and CRY, which inhibit their own transcriptional activators, CLOCK and BMAL1. Current models posit that CRY is the dominant repressor, while PER may play an accessory role. In this stu...

journal_title：Molecular cell

authors： Chen R,Schirmer A,Lee Y,Lee H,Kumar V,Yoo SH,Takahashi JS,Lee C

更新日期：2009-11-13 00:00:00

abstract：:Brr2 is a unique DExD/H box protein required for catalytic activation and disassembly of the spliceosome. It contains two tandem helicase cassettes that both comprise dual RecA-like domains and a noncanonical Sec63 unit. The latter may bestow the enzyme with unique properties. We have determined crystal structures of ...

journal_title：Molecular cell

authors： Pena V,Jovin SM,Fabrizio P,Orlowski J,Bujnicki JM,Lührmann R,Wahl MC

更新日期：2009-08-28 00:00:00

abstract：:Nhp6A and Nhp6B are HMG1-like proteins required for the growth of S. cerevisiae at elevated temperatures. We show that the conditional lethality of an nhp6 strain results from defective transcription of SNR6 (U6 snRNA) by RNA polymerase III. Overexpression of U6 snRNA or Brf1, a limiting component of TFIIIB, and an ac...

journal_title：Molecular cell

authors： Kruppa M,Moir RD,Kolodrubetz D,Willis IM

更新日期：2001-02-01 00:00:00

abstract：:Ubiquitin-mediated proteolysis regulates the activity of diverse receptor systems. Here, we identify Smurf2, a C2-WW-HECT domain ubiquitin ligase and show that Smurf2 associates constitutively with Smad7. Smurf2 is nuclear, but binding to Smad7 induces export and recruitment to the activated TGF beta receptor, where i...

journal_title：Molecular cell

authors： Kavsak P,Rasmussen RK,Causing CG,Bonni S,Zhu H,Thomsen GH,Wrana JL

更新日期：2000-12-01 00:00:00

abstract：:Mutation and prevalence of pathogenic viruses prompt the development of broad-spectrum antiviral strategies. Viperin is a potent antiviral protein that inhibits a broad range of viruses. Unexpectedly, we found that Viperin protein production in epithelium is defective in response to both viruses and interferons (IFNs)...

journal_title：Molecular cell

authors： Yuan Y,Miao Y,Qian L,Zhang Y,Liu C,Liu J,Zuo Y,Feng Q,Guo T,Zhang L,Chen X,Jin L,Huang F,Zhang H,Zhang W,Li W,Xu G,Zheng H

更新日期：2020-02-20 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptati...

journal_title：Molecular cell

authors： Sternberg SH,Richter H,Charpentier E,Qimron U

更新日期：2016-03-17 00:00:00

abstract：:Members of the ISWI family of chromatin remodeling factors exhibit ATP-dependent nucleosome sliding, loading, and spacing activities in vitro. However, it is unclear which of these activities are utilized by ISWI complexes to remodel chromatin in vivo. We therefore sought to identify the mechanisms of chromatin remode...

journal_title：Molecular cell

authors： Fazzio TG,Tsukiyama T

更新日期：2003-11-01 00:00:00

abstract：:A variety of debilitating diseases including diabetes, Alzheimer's, Huntington's, Parkinson's, and prion-based diseases are linked to stress within the endoplasmic reticulum (ER). Using S. cerevisiae, we sought to determine the relationship between protein misfolding, ER stress, and cell death. In the absence of ERV29...

journal_title：Molecular cell

authors： Haynes CM,Titus EA,Cooper AA

更新日期：2004-09-10 00:00:00