Abstract:
:The mechanisms controlling growth and patterning along the proximal-distal axis of the vertebrate limb are yet to be understood. We show that restriction of expression of the homeobox gene Meis2 to proximal regions of the limb bud is essential for limb development, since ectopic Meis2 severely disrupts limb outgrowth. We also uncover an antagonistic relationship between the secreted factors Gremlin and BMPs required to maintain the Shh/FGF loop that regulates distal outgrowth. These proximal and distal factors have coordinated activities: Meis2 can repress distal genes, and Bmps and Hoxd genes restrict Meis2 expression to the proximal limb bud. Moreover, combinations of BMPs and AER factors are sufficient to distalize proximal limb cells. Our results unveil a novel set of proximal-distal regulatory interactions that establish and maintain outgrowth of the vertebrate limb.
journal_name
Mol Celljournal_title
Molecular cellauthors
Capdevila J,Tsukui T,Rodríquez Esteban C,Zappavigna V,Izpisúa Belmonte JCdoi
10.1016/s1097-2765(00)80393-7subject
Has Abstractpub_date
1999-11-01 00:00:00pages
839-49issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80393-7journal_volume
4pub_type
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