Connecting replication and recombination.

abstract：:Cryo-EM structures of Dot1L in complex with a ubiquitinated nucleosome provide the long-sought-after molecular mechanism of Dot1L-mediated methylation of lysine 79 in histone H3 and explain crosstalk with histone H2B ubiquitination. ...

journal_title：Molecular cell

authors： Zhang Y,Kutateladze TG

更新日期：2019-06-06 00:00:00

abstract：:In this issue of Molecular Cell, Roh et al. (2018) present a high-resolution cryo-EM structure of the nanodisc-reconstituted yeast Vo proton channel that provides important new insights into subunit arrangements and the proton translocation pathway in V-type ATPases. ...

journal_title：Molecular cell

authors： Muench SP,van der Laan M

更新日期：2018-03-15 00:00:00

abstract：:The structure of an RNA polymerase II/general transcription factor TFIIF complex was determined by cryo-electron microscopy and single particle analysis. Density due to TFIIF was not concentrated in one area but rather was widely distributed across the surface of the polymerase. The largest subunit of TFIIF interacted...

journal_title：Molecular cell

authors： Chung WH,Craighead JL,Chang WH,Ezeokonkwo C,Bareket-Samish A,Kornberg RD,Asturias FJ

更新日期：2003-10-01 00:00:00

abstract：:Polyubiquitination is a posttranslational modification where ubiquitin chains containing isopeptide bonds linking one of seven ubiquitin lysines with the C terminus of an adjoining ubiquitin are covalently attached to proteins. While functions of K48- and K63-linked polyubiquitin are understood, the role(s) of noncano...

journal_title：Molecular cell

authors： Matsumoto ML,Wickliffe KE,Dong KC,Yu C,Bosanac I,Bustos D,Phu L,Kirkpatrick DS,Hymowitz SG,Rape M,Kelley RF,Dixit VM

更新日期：2010-08-13 00:00:00

abstract：:Protein synthesis is a major target within the bacterial cell for antibiotics. Investigations into ribosome-targeting antibiotics have provided much needed functional and structural insight into their mechanism of action. However, the increasing prevalence of multi-drug-resistant bacteria has limited the utility of ou...

journal_title：Molecular cell

authors： Arenz S,Wilson DN

更新日期：2016-01-07 00:00:00

abstract：:In this issue of Molecular Cell, Taneja et al. (2017) uncover a dual role for the conserved chromatin remodeler Fft3 in the maintenance of silent heterochromatin and the suppression of replication barriers at euchromatic loci through controlled histone turnover. ...

journal_title：Molecular cell

authors： Fortuny A,Polo SE

更新日期：2017-04-06 00:00:00

abstract：:In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D elect...

journal_title：Molecular cell

authors： Sander B,Golas MM,Makarov EM,Brahms H,Kastner B,Lührmann R,Stark H

更新日期：2006-10-20 00:00:00

abstract：:In Saccharomyces cerevisiae, telomere replication occurs in late S phase and is accompanied by dynamic remodeling of its protein components. Here, we show that MRX (Mre11-Rad50-Xrs2), an evolutionarily conserved protein complex involved in DNA double-strand break (DSB) repair, is recruited to the telomeres in late S p...

journal_title：Molecular cell

authors： Takata H,Tanaka Y,Matsuura A

更新日期：2005-02-18 00:00:00

abstract：:Nonsense-mediated decay (NMD) degrades mRNAs containing a premature termination codon (PTC). PTCs are a frequent cause of human genetic diseases, and the NMD pathway is known to modulate disease severity. Since partial NMD attenuation can potentially enhance nonsense suppression therapies, better definition of human-s...

journal_title：Molecular cell

authors： Alexandrov A,Shu MD,Steitz JA

更新日期：2017-01-05 00:00:00

abstract：:Fidaxomicin is an antibacterial drug in clinical use for treatment of Clostridium difficile diarrhea. The active ingredient of fidaxomicin, lipiarmycin A3 (Lpm), functions by inhibiting bacterial RNA polymerase (RNAP). Here we report a cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme in complex with Lpm...

journal_title：Molecular cell

authors： Lin W,Das K,Degen D,Mazumder A,Duchi D,Wang D,Ebright YW,Ebright RY,Sineva E,Gigliotti M,Srivastava A,Mandal S,Jiang Y,Liu Y,Yin R,Zhang Z,Eng ET,Thomas D,Donadio S,Zhang H,Zhang C,Kapanidis AN,Ebright RH

更新日期：2018-04-05 00:00:00

abstract：:tRNAs are subject to numerous modifications, including methylation. Mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation, yet the molecular and cellular function in mammals is not well understood. We developed m7G methylated tRNA immunop...

journal_title：Molecular cell

authors： Lin S,Liu Q,Lelyveld VS,Choe J,Szostak JW,Gregory RI

更新日期：2018-07-19 00:00:00

abstract：:The Latin word "facultas" literally means "opportunity." Facultative heterochromatin (fHC) then designates genomic regions in the nucleus of a eukaryotic cell that have the opportunity to adopt open or compact conformations within temporal and spatial contexts. This review focuses on the molecular and functional aspec...

journal_title：Molecular cell

authors： Trojer P,Reinberg D

更新日期：2007-10-12 00:00:00

abstract：:Bcl-2 proteins are critical regulators of apoptosis following DNA damage. Recent studies have shown that genotoxic stress induces the deamidation of Bcl-X(L), inhibiting its antiapoptotic activities. Remarkably, Rb and p53 are important regulators of this novel modification of Bcl-X(L). ...

journal_title：Molecular cell

authors： Johnstone RW

更新日期：2002-10-01 00:00:00

abstract：:Homologous recombination is essential for preserving genome integrity. Joining of homologous DNA molecules through strand exchange, a pivotal step in recombination, is mediated by RAD51. Here, we identify RAD51AP1 as a RAD51 accessory protein that specifically stimulates joint molecule formation through the combinatio...

journal_title：Molecular cell

authors： Modesti M,Budzowska M,Baldeyron C,Demmers JA,Ghirlando R,Kanaar R

更新日期：2007-11-09 00:00:00

abstract：:We have identified ARAP1 and ARAP2 and examined ARAP1 as a possible link between phosphoinositide-, Arf-, and Rho-mediated cell signaling. ARAP1 contains Arf GAP, Rho GAP, Ankyrin repeat, Ras-associating, and five PH domains. In vitro, ARAP1 had Rho GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent A...

journal_title：Molecular cell

authors： Miura K,Jacques KM,Stauffer S,Kubosaki A,Zhu K,Hirsch DS,Resau J,Zheng Y,Randazzo PA

更新日期：2002-01-01 00:00:00

abstract：:In a recent issue of Molecular Cell, Pidoux et al. (2009) and Williams et al. (2009) identify S. pombe Scm3 as the proximate factor in the Cnp1/CENP-A deposition pathway, providing a direct connection to centromere-localized Mis16-Mis18. ...

journal_title：Molecular cell

authors： Baker RE

更新日期：2009-02-27 00:00:00

abstract：:Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importi...

journal_title：Molecular cell

authors： Cingolani G,Bednenko J,Gillespie MT,Gerace L

更新日期：2002-12-01 00:00:00

abstract：:We analyzed the order of recruitment of factors to the HNF-4alpha regulatory regions upon the initial activation of the gene during enterocyte differentiation. An initially independent assembly of regulatory complexes at the proximal promoter and the upstream enhancer regions was followed by the tracking of the entire...

journal_title：Molecular cell

authors： Hatzis P,Talianidis I

更新日期：2002-12-01 00:00:00

abstract：:Eukaryotic transcription and mRNA processing depend upon the coordinated interactions of many proteins, including Spn1 and Spt6, which are conserved across eukaryotes, are essential for viability, and associate with each other in some of their biologically important contexts. Here we report crystal structures of the S...

journal_title：Molecular cell

authors： McDonald SM,Close D,Xin H,Formosa T,Hill CP

更新日期：2010-12-10 00:00:00

abstract：:The accurate propagation of histone marks during chromosomal replication is proposed to rely on the tight coupling of replication with the recycling of parental histones to the daughter strands. Here, we show in the avian cell line DT40 that REV1, a key regulator of DNA translesion synthesis at the replication fork, i...

journal_title：Molecular cell

authors： Sarkies P,Reams C,Simpson LJ,Sale JE

更新日期：2010-12-10 00:00:00

abstract：:The anaphase-promoting complex (APC) is a ubiquitin ligase that promotes the degradation of cell-cycle regulators. Cdh1p is an APC coactivator that directly binds APC substrates. A genetic screen in budding yeast identified residues within Cdh1p critical for its function. Cdh1p proteins containing mutations within the...

journal_title：Molecular cell

authors： Burton JL,Tsakraklides V,Solomon MJ

更新日期：2005-05-27 00:00:00

abstract：:The histone methyltransferase PR-Set7/Set8 is the sole enzyme that catalyzes monomethylation of histone H4 at K20 (H4K20me1). Previous reports document disparate evidence regarding PR-Set7 expression during the cell cycle, the biological relevance of PR-Set7 interaction with PCNA, and its role in the cell. We find tha...

journal_title：Molecular cell

authors： Oda H,Hübner MR,Beck DB,Vermeulen M,Hurwitz J,Spector DL,Reinberg D

更新日期：2010-11-12 00:00:00

abstract：:Mitochondrial diseases and aging are associated with defects in the oxidative phosphorylation machinery (OXPHOS), which are the only complexes composed of proteins encoded by separate genomes. To better understand genome coordination and OXPHOS recovery during mitochondrial dysfunction, we examined ATFS-1, a transcrip...

journal_title：Molecular cell

authors： Nargund AM,Fiorese CJ,Pellegrino MW,Deng P,Haynes CM

更新日期：2015-04-02 00:00:00

abstract：:Growing resistance of pathogenic bacteria and shortage of antibiotic discovery platforms challenge the use of antibiotics in the clinic. This threat calls for exploration of unconventional sources of antibiotics and identification of inhibitors able to eradicate resistant bacteria. Here we describe a different class o...

journal_title：Molecular cell

authors： Pantel L,Florin T,Dobosz-Bartoszek M,Racine E,Sarciaux M,Serri M,Houard J,Campagne JM,de Figueiredo RM,Midrier C,Gaudriault S,Givaudan A,Lanois A,Forst S,Aumelas A,Cotteaux-Lautard C,Bolla JM,Vingsbo Lundberg C,Huseby

更新日期：2018-04-05 00:00:00

abstract：:Homology-dependent repair of double-strand breaks (DSBs) from nonsister templates has the potential to generate loss of heterozygosity or genome rearrangements. Here we show that the Saccharomyces cerevisiae Mph1 helicase prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10...

journal_title：Molecular cell

authors： Mazón G,Symington LS

更新日期：2013-10-10 00:00:00

abstract：:The RNA exosome complex associates with nuclear and cytoplasmic cofactors to mediate the decay, surveillance, or processing of a wide variety of transcripts. In the cytoplasm, the conserved core of the exosome (Exo10) functions together with the conserved Ski complex. The interaction of S. cerevisiae Exo10 and Ski is ...

journal_title：Molecular cell

authors： Kowalinski E,Kögel A,Ebert J,Reichelt P,Stegmann E,Habermann B,Conti E

更新日期：2016-07-07 00:00:00

abstract：:The intracellular regulation of cell death pathways by cIAPs has been enigmatic. Here we show that loss of cIAPs promotes the spontaneous formation of an intracellular platform that activates either apoptosis or necroptosis. This 2 MDa intracellular complex that we designate "Ripoptosome" is necessary but not sufficie...

journal_title：Molecular cell

authors： Feoktistova M,Geserick P,Kellert B,Dimitrova DP,Langlais C,Hupe M,Cain K,MacFarlane M,Häcker G,Leverkus M

更新日期：2011-08-05 00:00:00

abstract：:The membrane-spanning C-terminal regions in tail-anchored proteins must be recognized and delivered posttranslationally to the endoplasmic reticulum or mitochondrial membrane. A paper in this issue of Molecular Cell (Wang et al., 2010) and another recent report (Mariappan et al., 2010) delineate early steps in this pa...

journal_title：Molecular cell

authors： Brodsky JL

更新日期：2010-10-08 00:00:00

abstract：:Streptolydigin (Stl) is a potent inhibitor of bacterial RNA polymerases (RNAPs). The 2.4 A resolution structure of the Thermus thermophilus RNAP-Stl complex showed that, in full agreement with the available genetic data, the inhibitor binding site is located 20 A away from the RNAP active site and encompasses the brid...

journal_title：Molecular cell

authors： Temiakov D,Zenkin N,Vassylyeva MN,Perederina A,Tahirov TH,Kashkina E,Savkina M,Zorov S,Nikiforov V,Igarashi N,Matsugaki N,Wakatsuki S,Severinov K,Vassylyev DG

更新日期：2005-09-02 00:00:00

abstract：:ClpXP is a protease involved in DNA damage repair, stationary-phase gene expression, and ssrA-mediated protein quality control. To date, however, only a handful of ClpXP substrates have been identified. Using a tagged and inactive variant of ClpP, substrates of E. coli ClpXP were trapped in vivo, purified, and identif...

journal_title：Molecular cell

authors： Flynn JM,Neher SB,Kim YI,Sauer RT,Baker TA

更新日期：2003-03-01 00:00:00