Abstract:
:We analyzed the order of recruitment of factors to the HNF-4alpha regulatory regions upon the initial activation of the gene during enterocyte differentiation. An initially independent assembly of regulatory complexes at the proximal promoter and the upstream enhancer regions was followed by the tracking of the entire DNA-protein complex formed on the enhancer along the intervening DNA until it encountered the proximal promoter. This movement correlated with a unidirectional spreading of histone hyperacetylation. Transcription initiation coincided with the formation of a stable enhancer-promoter complex and remodeling of the nucleosome situated at the transcription start site. The results provide experimental evidence for the involvement of a dynamic process culminating in enhancer-promoter communication during long-distance gene activation.
journal_name
Mol Celljournal_title
Molecular cellauthors
Hatzis P,Talianidis Idoi
10.1016/s1097-2765(02)00786-4subject
Has Abstractpub_date
2002-12-01 00:00:00pages
1467-77issue
6eissn
1097-2765issn
1097-4164pii
S1097-2765(02)00786-4journal_volume
10pub_type
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