Abstract：
:Homologous recombination is essential for preserving genome integrity. Joining of homologous DNA molecules through strand exchange, a pivotal step in recombination, is mediated by RAD51. Here, we identify RAD51AP1 as a RAD51 accessory protein that specifically stimulates joint molecule formation through the combination of structure-specific DNA binding and physical contact with RAD51. At the cellular level, we show that RAD51AP1 is required to protect cells from the adverse effects of DNA double-strand break-inducing agents. At the biochemical level, we show that RAD51AP1 has a selective affinity for branched-DNA structures that are obligatory intermediates during joint molecule formation. Our results highlight the importance of structural transitions in DNA as control points in recombination. The affinity of RAD51AP1 for the central protein and DNA intermediates of recombination confers on it the ability to control the preservation of genome integrity at a number of critical mechanistic steps.
journal_name
Mol Celljournal_title
Molecular cellauthors
Modesti M,Budzowska M,Baldeyron C,Demmers JA,Ghirlando R,Kanaar Rdoi
10.1016/j.molcel.2007.08.025subject
Has Abstractpub_date
2007-11-09 00:00:00pages
468-81issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(07)00595-3journal_volume
28pub_type
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