当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore.

The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore.

Abstract：

:Nuclear export of mRNA in eukaryotic cells is mediated by soluble transport factors and components of the nuclear pore complex (NPC). The cytoplasmically oriented nuclear pore protein Nup159 plays a critical role in mRNA export through its conserved N-terminal domain (NTD). Here, we report the crystal structure of the Nup159 NTD, refined to 2.5 A. The structure reveals an unusually asymmetric seven-bladed beta-propeller that is structurally conserved throughout eukarya. Using structure-based conservation analysis, we have targeted specific surface residues for mutagenesis. Residue substitutions in a conserved loop of the NTD abolish in vitro binding to Dbp5, a DEAD box helicase required for mRNA export. In vivo, these mutations cause Dbp5 mislocalization and block mRNA export. These findings suggest that the Nup159 NTD functions in mRNA export as a binding platform, tethering shuttling Dbp5 molecules at the nuclear periphery and locally concentrating this mRNA remodeling factor at the cytoplasmic face of the NPC.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Weirich CS,Erzberger JP,Berger JM,Weis K

doi

10.1016/j.molcel.2004.10.032

subject

Has Abstract

pub_date

2004-12-03 00:00:00

pages

749-60

issue

5

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(04)00652-5

journal_volume

16

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • cIAPs block Ripoptosome formation, a RIP1/caspase-8 containing intracellular cell death complex differentially regulated by cFLIP isoforms.

    abstract：:The intracellular regulation of cell death pathways by cIAPs has been enigmatic. Here we show that loss of cIAPs promotes the spontaneous formation of an intracellular platform that activates either apoptosis or necroptosis. This 2 MDa intracellular complex that we designate "Ripoptosome" is necessary but not sufficie...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.06.011

    authors： Feoktistova M,Geserick P,Kellert B,Dimitrova DP,Langlais C,Hupe M,Cain K,MacFarlane M,Häcker G,Leverkus M

    更新日期：2011-08-05 00:00:00

  • Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes.

    abstract：:Inappropriate activation of oncogenic kinases at intracellular locations is frequently observed in human cancers, but its effects on global signaling are incompletely understood. Here, we show that the oncogenic mutant of Flt3 (Flt3-ITD), when localized at the endoplasmic reticulum (ER), aberrantly activates STAT5 and...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.09.019

    authors： Choudhary C,Olsen JV,Brandts C,Cox J,Reddy PN,Böhmer FD,Gerke V,Schmidt-Arras DE,Berdel WE,Müller-Tidow C,Mann M,Serve H

    更新日期：2009-10-23 00:00:00

  • Transcriptional elongation factor ENL phosphorylated by ATM recruits polycomb and switches off transcription for DSB repair.

    abstract：:Transcription is repressed if a DNA double-strand break (DSB) is introduced in close proximity to a transcriptional activation site at least in part by H2A-ubiquitination. While ATM signaling is involved, how it controls H2A-ubiquitination remains unclear. Here, we identify that, in response to DSBs, a transcriptional...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.03.023

    authors： Ui A,Nagaura Y,Yasui A

    更新日期：2015-05-07 00:00:00

  • Molecular mechanism and evolution of guanylate kinase regulation by (p)ppGpp.

    abstract：:The nucleotide (p)ppGpp mediates bacterial stress responses, but its targets and underlying mechanisms of action vary among bacterial species and remain incompletely understood. Here, we characterize the molecular interaction between (p)ppGpp and guanylate kinase (GMK), revealing the importance of this interaction in ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.12.037

    authors： Liu K,Myers AR,Pisithkul T,Claas KR,Satyshur KA,Amador-Noguez D,Keck JL,Wang JD

    更新日期：2015-02-19 00:00:00

  • Linkage-specific avidity defines the lysine 63-linked polyubiquitin-binding preference of rap80.

    abstract：:Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.02.011

    authors： Sims JJ,Cohen RE

    更新日期：2009-03-27 00:00:00

  • The heat shock response: life on the verge of death.

    abstract：:Organisms must survive a variety of stressful conditions, including sudden temperature increases that damage important cellular structures and interfere with essential functions. In response to heat stress, cells activate an ancient signaling pathway leading to the transient expression of heat shock or heat stress pro...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2010.10.006

    authors： Richter K,Haslbeck M,Buchner J

    更新日期：2010-10-22 00:00:00

  • Transcriptional regulation by p53 through intrinsic DNA/chromatin binding and site-directed cofactor recruitment.

    abstract：:The tumor suppressor protein, p53, plays a critical role in mediating cellular response to stress signals by regulating genes involved in cell cycle arrest and apoptosis. p53 is believed to be inactive for DNA binding unless its C terminus is modified or structurally altered. We show that unmodified p53 actively binds...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(01)00283-0

    authors： Espinosa JM,Emerson BM

    更新日期：2001-07-01 00:00:00

  • Conserved GU-rich elements mediate mRNA decay by binding to CUG-binding protein 1.

    abstract：:We used computational algorithms to find conserved sequences in the 3' untranslated region (UTR) of transcripts that exhibited rapid decay in primary human T cells and found that the consensus sequence UGUUUGUUUGU, which we have termed a GU-rich element (GRE), was enriched in short-lived transcripts. Using a tet-off r...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.11.024

    authors： Vlasova IA,Tahoe NM,Fan D,Larsson O,Rattenbacher B,Sternjohn JR,Vasdewani J,Karypis G,Reilly CS,Bitterman PB,Bohjanen PR

    更新日期：2008-02-01 00:00:00

  • How Rho exerts its muscle on RNA.

    abstract：:Rho factor in bacteria terminates transcription by using energy from ATP hydrolysis to forcefully dissociate the transcripts from RNA polymerase. used data from presteady-state ATPase kinetics to support a rational mechanistic model for Rho's action on RNA. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章,评审

    doi：10.1016/j.molcel.2006.06.002

    authors： Richardson JP

    更新日期：2006-06-23 00:00:00

  • Functional interdependence at the chromatin level between the MKK6/p38 and IGF1/PI3K/AKT pathways during muscle differentiation.

    abstract：:During muscle regeneration, the mechanism integrating environmental cues at the chromatin of muscle progenitors is unknown. We show that inflammation-activated MKK6-p38 and insulin growth factor 1 (IGF1)-induced PI3K/AKT pathways converge on the chromatin of muscle genes to target distinct components of the muscle tra...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.08.021

    authors： Serra C,Palacios D,Mozzetta C,Forcales SV,Morantte I,Ripani M,Jones DR,Du K,Jhala US,Simone C,Puri PL

    更新日期：2007-10-26 00:00:00

  • Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication.

    abstract：:Gene-targeted knockout mice have been generated lacking the major uracil-DNA glycosylase, UNG. In contrast to ung- mutants of bacteria and yeast, such mice do not exhibit a greatly increased spontaneous mutation frequency. However, there is only slow removal of uracil from misincorporated dUMP in isolated ung-/- nucle...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80271-3

    authors： Nilsen H,Rosewell I,Robins P,Skjelbred CF,Andersen S,Slupphaug G,Daly G,Krokan HE,Lindahl T,Barnes DE

    更新日期：2000-06-01 00:00:00

  • MOF-regulated acetylation of MSL-3 in the Drosophila dosage compensation complex.

    abstract：:Dosage compensation ensures equal expression of X-linked genes in males and females. In Drosophila, equalization is achieved by hypertranscription of the male X chromosome. This process requires an RNA/protein containing dosage compensation complex (DCC). Here we use RNA interference of individual DCC components to de...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00140-0

    authors： Buscaino A,Köcher T,Kind JH,Holz H,Taipale M,Wagner K,Wilm M,Akhtar A

    更新日期：2003-05-01 00:00:00

  • Essential role of ERK dimers in the activation of cytoplasmic but not nuclear substrates by ERK-scaffold complexes.

    abstract：:Signals transmitted by ERK MAP kinases regulate the functions of multiple substrates present in the nucleus and in the cytoplasm. ERK signals are optimized by scaffold proteins that modulate their intensity and spatial fidelity. Once phosphorylated, ERKs dimerize, but how dimerization impacts on the activation of the ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.07.024

    authors： Casar B,Pinto A,Crespo P

    更新日期：2008-09-05 00:00:00

  • A Multiplexed Assay for Exon Recognition Reveals that an Unappreciated Fraction of Rare Genetic Variants Cause Large-Effect Splicing Disruptions.

    abstract：:Mutations that lead to splicing defects can have severe consequences on gene function and cause disease. Here, we explore how human genetic variation affects exon recognition by developing a multiplexed functional assay of splicing using Sort-seq (MFASS). We assayed 27,733 variants in the Exome Aggregation Consortium ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.10.037

    authors： Cheung R,Insigne KD,Yao D,Burghard CP,Wang J,Hsiao YE,Jones EM,Goodman DB,Xiao X,Kosuri S

    更新日期：2019-01-03 00:00:00

  • Single-Cell Alternative Splicing Analysis with Expedition Reveals Splicing Dynamics during Neuron Differentiation.

    abstract：:Alternative splicing (AS) generates isoform diversity for cellular identity and homeostasis in multicellular life. Although AS variation has been observed among single cells, little is known about the biological or evolutionary significance of such variation. We developed Expedition, a computational framework consisti...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.06.003

    authors： Song Y,Botvinnik OB,Lovci MT,Kakaradov B,Liu P,Xu JL,Yeo GW

    更新日期：2017-07-06 00:00:00

  • Sam68 is required for both NF-κB activation and apoptosis signaling by the TNF receptor.

    abstract：:The RNA-binding protein Sam68 is implicated in various cellular processes including RNA metabolism, apoptosis, and signal transduction. Here we identify a role of Sam68 in TNF-induced NF-κB activation and apoptosis. We found that Sam68 is recruited to the TNF receptor, and its deficiency dramatically reduces RIP recru...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.05.007

    authors： Ramakrishnan P,Baltimore D

    更新日期：2011-07-22 00:00:00

  • Chaperonin TRiC promotes the assembly of polyQ expansion proteins into nontoxic oligomers.

    abstract：:Aberrant folding and fibrillar aggregation by polyglutamine (polyQ) expansion proteins are associated with cytotoxicity in Huntington's disease and other neurodegenerative disorders. Hsp70 chaperones have an inhibitory effect on fibril formation and can alleviate polyQ cytotoxicity. Here we show that the cytosolic cha...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.08.017

    authors： Behrends C,Langer CA,Boteva R,Böttcher UM,Stemp MJ,Schaffar G,Rao BV,Giese A,Kretzschmar H,Siegers K,Hartl FU

    更新日期：2006-09-15 00:00:00

  • The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase.

    abstract：:The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unk...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(02)00473-2

    authors： Sumara I,Vorlaufer E,Stukenberg PT,Kelm O,Redemann N,Nigg EA,Peters JM

    更新日期：2002-03-01 00:00:00

  • Hierarchical regulation of WASP/WAVE proteins.

    abstract：:Members of the Wiskott-Aldrich syndrome protein (WASP) family control actin dynamics in eukaryotic cells by stimulating the actin nucleating activity of the Arp2/3 complex. The prevailing paradigm for WASP regulation invokes allosteric relief of autoinhibition by diverse upstream activators. Here we demonstrate an add...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.10.012

    authors： Padrick SB,Cheng HC,Ismail AM,Panchal SC,Doolittle LK,Kim S,Skehan BM,Umetani J,Brautigam CA,Leong JM,Rosen MK

    更新日期：2008-11-07 00:00:00

  • Structure and dynamics of the mammalian ribosomal pretranslocation complex.

    abstract：:Although the structural core of the ribosome is conserved in all kingdoms of life, eukaryotic ribosomes are significantly larger and more complex than their bacterial counterparts. The extent to which these differences influence the molecular mechanism of translation remains elusive. Multiparticle cryo-electron micros...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.07.040

    authors： Budkevich T,Giesebrecht J,Altman RB,Munro JB,Mielke T,Nierhaus KH,Blanchard SC,Spahn CM

    更新日期：2011-10-21 00:00:00

  • The Biogenesis, Functions, and Challenges of Circular RNAs.

    abstract：:Covalently closed circular RNAs (circRNAs) are produced by precursor mRNA back-splicing of exons of thousands of genes in eukaryotes. circRNAs are generally expressed at low levels and often exhibit cell-type-specific and tissue-specific patterns. Recent studies have shown that their biogenesis requires spliceosomal m...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2018.06.034

    authors： Li X,Yang L,Chen LL

    更新日期：2018-08-02 00:00:00

  • Destabilizing LSD1 by Jade-2 promotes neurogenesis: an antibraking system in neural development.

    abstract：:Histone H3K4 demethylase LSD1 plays an important role in stem cell biology, especially in the maintenance of the silencing of differentiation genes. However, how the function of LSD1 is regulated and the differentiation genes are derepressed are not understood. Here, we report that elimination of LSD1 promotes embryon...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.06.006

    authors： Han X,Gui B,Xiong C,Zhao L,Liang J,Sun L,Yang X,Yu W,Si W,Yan R,Yi X,Zhang D,Li W,Li L,Yang J,Wang Y,Sun YE,Zhang D,Meng A,Shang Y

    更新日期：2014-08-07 00:00:00

  • The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway.

    abstract：:Translational control is frequently exerted at the stage of mRNA recruitment to the initiating ribosome. We have reconstituted mRNA recruitment to the 43S preinitiation complex (PIC) using purified S. cerevisiae components. We show that eIF3 and the eIF4 factors not only stabilize binding of mRNA to the PIC, they also...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.08.021

    authors： Mitchell SF,Walker SE,Algire MA,Park EH,Hinnebusch AG,Lorsch JR

    更新日期：2010-09-24 00:00:00

  • RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination.

    abstract：:Homologous recombination is essential for preserving genome integrity. Joining of homologous DNA molecules through strand exchange, a pivotal step in recombination, is mediated by RAD51. Here, we identify RAD51AP1 as a RAD51 accessory protein that specifically stimulates joint molecule formation through the combinatio...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.08.025

    authors： Modesti M,Budzowska M,Baldeyron C,Demmers JA,Ghirlando R,Kanaar R

    更新日期：2007-11-09 00:00:00

  • Engineering biological systems with synthetic RNA molecules.

    abstract：:RNA molecules play diverse functional roles in natural biological systems. There has been growing interest in designing synthetic RNA counterparts for programming biological function. The design of synthetic RNA molecules that exhibit diverse activities, including sensing, regulatory, information processing, and scaff...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2011.08.023

    authors： Liang JC,Bloom RJ,Smolke CD

    更新日期：2011-09-16 00:00:00

  • VSV disrupts the Rae1/mrnp41 mRNA nuclear export pathway.

    abstract：:Interference with nucleocytoplasmic transport is a strategy employed by certain viruses to compromise host cellular function. While it has been shown that the matrix (M) protein of the vesicular stomatitis virus (VSV) inhibits nuclear export of host cell mRNAs, the underlying mechanism has not been fully established. ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.11.023

    authors： Faria PA,Chakraborty P,Levay A,Barber GN,Ezelle HJ,Enninga J,Arana C,van Deursen J,Fontoura BM

    更新日期：2005-01-07 00:00:00

  • E1 on the move.

    abstract：:E1 enzymes activate ubiquitin or related proteins and pass them to E2 enzymes. A recent structure and associated biochemical studies (Huang et al., 2005) show how an E1 binds its cognate E2 and indicates that large, conformational changes will be an integral component of the E1 reaction cycle. ...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2005.02.004

    authors： VanDemark AP,Hill CP

    更新日期：2005-02-18 00:00:00

  • The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells.

    abstract：:Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Unbiased detection through proximity labeling ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2020.06.014

    authors： Wang AS,Chen LC,Wu RA,Hao Y,McSwiggen DT,Heckert AB,Richardson CD,Gowen BG,Kazane KR,Vu JT,Wyman SK,Shin JJ,Darzacq X,Walter JC,Corn JE

    更新日期：2020-07-16 00:00:00

  • Cell signaling and function organized by PB1 domain interactions.

    abstract：:The PB1-domain-containing proteins p62, aPKC, MEKK2/MEKK3, MEK5, and Par-6 play roles in critical cell processes like osteoclastogenesis, angiogenesis, and early cardiovascular development or cell polarity. PB1 domains are scaffold modules that adopt the topology of ubiquitin-like beta-grasp folds that interact with e...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2006.08.002

    authors： Moscat J,Diaz-Meco MT,Albert A,Campuzano S

    更新日期：2006-09-01 00:00:00

  • SLC19A1 Is an Importer of the Immunotransmitter cGAMP.

    abstract：:2'3'-cyclic-GMP-AMP (cGAMP) is a second messenger that activates the antiviral stimulator of interferon genes (STING) pathway. We recently identified a novel role for cGAMP as a soluble, extracellular immunotransmitter that is produced and secreted by cancer cells. Secreted cGAMP is then sensed by host cells, elicitin...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2019.05.006

    authors： Ritchie C,Cordova AF,Hess GT,Bassik MC,Li L

    更新日期：2019-07-25 00:00:00