Abstract：
:The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unknown mechanism that does not involve cohesin cleavage. We show that Polo-like kinase is required for the cleavage-independent dissociation of cohesin from chromosomes in Xenopus. Cohesin phosphorylation depends on Polo-like kinase and reduces the ability of cohesin to bind to chromatin. These results suggest that Polo-like kinase regulates the dissociation of cohesin from chromosomes early in mitosis.
journal_name
Mol Celljournal_title
Molecular cellauthors
Sumara I,Vorlaufer E,Stukenberg PT,Kelm O,Redemann N,Nigg EA,Peters JMdoi
10.1016/s1097-2765(02)00473-2subject
Has Abstractpub_date
2002-03-01 00:00:00pages
515-25issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(02)00473-2journal_volume
9pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:The ability to measure human aging from molecular profiles has practical implications in many fields, including disease prevention and treatment, forensics, and extension of life. Although chronological age has been linked to changes in DNA methylation, the methylome has not yet been used to measure and compare human ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.10.016
更新日期：2013-01-24 00:00:00
abstract：:In vitro, the anaphase-promoting complex (APC) E3 ligase functions with E2 ubiquitin-conjugating enzymes of the E2-C and Ubc4/5 families to ubiquitinate substrates. However, only the use of the E2-C family, notably UbcH10, is genetically well validated. Here, we biochemically demonstrate preferential use of UbcH10 by ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2008.07.014
更新日期：2008-08-22 00:00:00
abstract：:In this issue of Molecular Cell, detail molecular events that trigger apoptosis following growth factor withdrawal, linking irreversible mitochondrial permeabilization to activation of GSK-3 and subsequent phosphorylation, ubiquitinylation, and degradation of the antiapoptotic BCL-2 family member MCL-1. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.molcel.2006.03.005
更新日期：2006-03-17 00:00:00
abstract：:Mutations within BRCA1 predispose carriers to a high risk of breast and ovarian cancers. BRCA1 functions to maintain genomic stability through the assembly of multiple protein complexes involved in DNA repair, cell-cycle arrest, and transcriptional regulation. Here, we report the identification of a DNA damage-induced...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2014.03.021
更新日期：2014-05-08 00:00:00
abstract：:Comparisons of bacteriophage PRD1 and adenovirus protein structures and virion architectures have been instrumental in unraveling an evolutionary relationship and have led to a proposal of a phylogeny-based virus classification. The structure of the PRD1 spike protein P5 provides further insight into the evolution of ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.03.019
更新日期：2005-04-15 00:00:00
abstract：:Proteins are translocated across membranes through a channel that is formed by the prokaryotic SecY or eukaryotic Sec61 complex. The crystal structure of the SecY channel from M. jannaschii revealed a plug domain that appears to seal the channel in its closed state. However, the role of the plug remains unclear, parti...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.05.002
更新日期：2007-05-25 00:00:00
abstract：:Cotranscriptional loading of proteins onto nascent transcripts contributes to the formation of messenger ribonucleoprotein particles (mRNPs) competent for nuclear export. The transcription machinery is believed to play a pivotal role in mRNP assembly, which is at least partially linked to the function of the THO/TREX ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2004.09.019
更新日期：2004-10-22 00:00:00
abstract：:Polycomb group (PcG) proteins exert essential functions in the most disparate biological processes. The contribution of PcG proteins to cell commitment and differentiation relates to their ability to repress transcription of developmental regulators in embryonic stem (ES) cells and in committed cell lineages, includin...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.08.008
更新日期：2009-10-09 00:00:00
abstract：:The Mre11-Rad50-Xrs2 (MRX) complex is related to SMC complexes that form rings capable of holding two distinct DNA strands together. MRX functions at stalled replication forks and double-strand breaks (DSBs). A mutation in the N-terminal OB fold of the 70 kDa subunit of yeast replication protein A, rfa1-t11, abrogates...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.10.032
更新日期：2016-12-01 00:00:00
abstract：:In Saccharomyces cerevisiae, telomere replication occurs in late S phase and is accompanied by dynamic remodeling of its protein components. Here, we show that MRX (Mre11-Rad50-Xrs2), an evolutionarily conserved protein complex involved in DNA double-strand break (DSB) repair, is recruited to the telomeres in late S p...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.01.014
更新日期：2005-02-18 00:00:00
abstract：:CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.12.015
更新日期：2019-02-21 00:00:00
abstract：:How polycomb group proteins repress gene expression in vivo is not known. While histone-modifying activities of the polycomb repressive complexes (PRCs) have been studied extensively, in vitro data have suggested a direct activity of the PRC1 complex in compacting chromatin. Here, we investigate higher-order chromatin...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.02.032
更新日期：2010-05-14 00:00:00
abstract：:The oncogenic process often leads to a loss of normal telomere length control, usually as a result of activation of telomerase. Nevertheless, there are also telomerase-independent events that involve a Rad50-dependent recombination mechanism to maintain telomere length. Previous work has implicated the Rb family of pr...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.02.016
更新日期：2006-04-07 00:00:00
abstract：:The Cdc28p cyclin-dependent kinase is thought to both catalyze the onset of DNA replication and prevent rereplication by blocking the reassembly of initiation complexes at replication origins. Budding yeast with mutations in the CDC16 gene represent an exception to this model, because they rereplicate DNA despite bein...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80046-5
更新日期：1998-02-01 00:00:00
abstract：:Networks of protein interactions coordinate cellular functions. We describe a bimolecular fluorescence complementation (BiFC) assay for determination of the locations of protein interactions in living cells. This approach is based on complementation between two nonfluorescent fragments of the yellow fluorescent protei...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00496-3
更新日期：2002-04-01 00:00:00
abstract：:Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importi...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00727-x
更新日期：2002-12-01 00:00:00
abstract：:There are many regulated steps in the assembly of a transcription preinitiation complex (PIC). In this issue of Molecular Cell, Black et. al. (2006) reveal a catalytic switch mechanism in which autoacetylation of the HAT p300 triggers its dissociation from a promoter in a manner that is coupled to TFIID association. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.molcel.2006.08.022
更新日期：2006-09-15 00:00:00
abstract：:Tumor suppressor genes evolved as negative effectors of mitogen and nutrient signaling pathways, such that mutations in these genes can lead to pathological states of growth. Tuberous sclerosis (TSC) is a potentially devastating disease associated with mutations in two tumor suppressor genes, TSC1 and 2, that function...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00220-x
更新日期：2003-06-01 00:00:00
abstract：:To unravel the roles of LXRs in inflammation and immunity, we examined the function of LXRs in development of IFN-gamma-mediated inflammation using cultured rat brain astrocytes. LXR ligands inhibit neither STAT1 phosphorylation nor STAT1 translocation to the nucleus but, rather, inhibit STAT1 binding to promoters and...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.07.021
更新日期：2009-09-24 00:00:00
abstract：:The methylcytosine oxidase TET proteins play important roles in DNA demethylation and development. However, it remains elusive how exactly they target substrates and execute oxidation. Interestingly, we found that, in mice, the full-length TET1 isoform (TET1e) is restricted to early embryos, embryonic stem cells (ESCs...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.10.030
更新日期：2016-12-15 00:00:00
abstract：:The subclass of cysteine proteases termed lysosomal cathepsins has long been thought to be primarily involved in end-stage protein breakdown within lysosomal compartments. Furthermore, few specific protein substrates for these proteases have been identified. We show here that cathepsin L functions in the regulation of...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(04)00209-6
更新日期：2004-04-23 00:00:00
abstract：:In this issue, Roberts et al. (2014) describe how hexokinase and mTORC1 cooperate to sense disequilibrium between glucose uptake and utilization and direct the balance of anabolism and catabolism to ensure the appropriate use of cellular resources. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2014.02.009
更新日期：2014-02-20 00:00:00
abstract：:Forkhead box O (FOXO) transcription factors, the key regulators of cell survival, are negatively controlled through the PI3K-Akt signaling pathway. Phosphorylation of FOXO by Akt leads to cytoplasmic localization and subsequent degradation via the ubiquitin-proteasome system. Here we show a paradigm of FOXO1 regulatio...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2008.09.013
更新日期：2008-10-24 00:00:00
abstract：:Solving the biological roles of covalent histone modifications, including monoubiquitination of histone H2A, and the molecular mechanisms by which these modifications regulate specific transcriptional programs remains a central question for all eukaryotes. Here we report that the N-CoR/HDAC1/3 complex specifically rec...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.11.002
更新日期：2008-01-18 00:00:00
abstract：:In this issue of Molecular Cell, Lee et al. (2009) report that SUMOylation of nuclear receptors LXRalpha and LXRbeta plays a critical role in the transrepression of IFN-gamma-induced STAT1-dependent inflammatory responses in brain astrocytes. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2009.09.002
更新日期：2009-09-24 00:00:00
abstract：:When inappropriate DNA structures arise, they are sensed by DNA structure-dependent checkpoint pathways and subsequently repaired. Recruitment of checkpoint proteins to such structures precedes recruitment of proteins involved in DNA metabolism. Thus, checkpoints can regulate DNA metabolism. We show that fission yeast...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.10.026
更新日期：2010-11-24 00:00:00
abstract：:Argonaute proteins are required for the biogenesis of some small RNAs (sRNAs), including the PIWI-interacting RNAs and some microRNAs. How Argonautes mediate maturation of sRNAs independent of their slicer activity is not clear. The maturation of the Neurospora microRNA-like sRNA, milR-1, requires the Argonaute protei...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.03.019
更新日期：2012-05-11 00:00:00
abstract：:In this issue of Molecular Cell, Conaway and colleagues (Yao et al., 2008) provide a glimpse into an interesting mechanism to control a deubiquitylating enzyme via interaction of two complexes-the 19S proteasome regulatory particle and an ATP-dependent nucleosome remodeling complex. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2008.09.005
更新日期：2008-09-26 00:00:00
abstract：:APOBEC1 is the catalytic component of an RNA editing complex but shows homology to activation-induced cytidine deaminase (AID), a protein whose function is to potentiate diversification of immunoglobulin gene DNA. Here, we show that APOBEC1 and its homologs APOBEC3C and APOBEC3G exhibit potent DNA mutator activity in ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00742-6
更新日期：2002-11-01 00:00:00
abstract：:We demonstrate that the major in vivo targets of brefeldin A (BFA) in the secretory pathway of budding yeast are the three members of the Sec7 domain family of ARF exchange factors: Gea1p and Gea2p (functionally interchangeable) and Sec7p. Specific residues within the Sec7 domain are important for BFA inhibition of AR...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80455-4
更新日期：1999-03-01 00:00:00
