Abstract：
:In Saccharomyces cerevisiae, telomere replication occurs in late S phase and is accompanied by dynamic remodeling of its protein components. Here, we show that MRX (Mre11-Rad50-Xrs2), an evolutionarily conserved protein complex involved in DNA double-strand break (DSB) repair, is recruited to the telomeres in late S phase. MRX is required for the late S phase-specific recruitment of ATR-like kinase Mec1 to the telomeres. Mec1, in turn, contributes to the assembly of the telomerase regulators Cdc13 and Est1 at the telomere ends. Our results provide a model for the hierarchical assembly of telomere-replication proteins in late S phase; this involves triggering by the loading of MRX onto the chromosome termini. The recruitment of DNA repair-related proteins to the telomeres at particular times in the cell cycle suggests that the normal terminus of a chromosome is recognized as a DSB during the course of replication.
journal_name
Mol Celljournal_title
Molecular cellauthors
Takata H,Tanaka Y,Matsuura Adoi
10.1016/j.molcel.2005.01.014subject
Has Abstractpub_date
2005-02-18 00:00:00pages
573-83issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(05)01050-6journal_volume
17pub_type
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