当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > The response regulator BvgA and RNA polymerase alpha subunit C-terminal domain bind simultaneously to different faces of the same segment of promoter DNA.

The response regulator BvgA and RNA polymerase alpha subunit C-terminal domain bind simultaneously to different faces of the same segment of promoter DNA.

Abstract：

:Examination of the binding of FeBABE-conjugated BvgA to the fha promoter of Bordetella pertussis has revealed that three dimers, formed by head-to-head association of monomers, bind one face of the DNA helix from the inverted-heptad primary binding site to the -35 region. The orientation of BvgA monomers within the dimers is the same as that recently demonstrated by X-ray crystallographic methods for a dimer of the C-terminal domain of NarL bound to DNA. Use of FeBABE conjugates of RNAP alpha subunit C-terminal domain showed that binding of this domain is linearly coincident with binding of the BvgA dimers, but to a different helical face. These results reveal a previously undescribed mode of interaction between RNAP alpha-CTD and a transcriptional activator.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Boucher PE,Maris AE,Yang MS,Stibitz S

doi

10.1016/s1097-2765(03)00007-8

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

163-73

issue

1

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(03)00007-8

journal_volume

11

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • Essential role for the mammalian ATG8 isoform LC3C in xenophagy.

    abstract：:In this issue, Randow and coworkers (von Muhlinen et al., 2012) report that LC3C plays an essential role in Salmonella clearance by interacting with the autophagic receptor NDP52, with consequent recruitment of all other ATG8 proteins to assist in the buildup of the autophagic membrane. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2012.10.020

    authors： Shpilka T,Elazar Z

    更新日期：2012-11-09 00:00:00

  • A bitter PP1 fights the sweet polo.

    abstract：:In a recent paper in Developmental Cell, Yamashiro et al. (2008) report that the PP1 regulatory subunit MYPT1 interacts with PLK1 and antagonizes essential mitotic functions of PLK1, at least in part by promoting the dephosphorylation of PLK1 at Thr210. ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.05.012

    authors： Archambault V,Chen F,Glover DM

    更新日期：2008-06-06 00:00:00

  • Structure of the Siz/PIAS SUMO E3 ligase Siz1 and determinants required for SUMO modification of PCNA.

    abstract：:Siz1 is a founding member of the Siz/PIAS RING family of SUMO E3 ligases. The X-ray structure of an active Siz1 ligase revealed an elongated tripartite architecture comprised of an N-terminal PINIT domain, a central zinc-containing RING-like SP-RING domain, and a C-terminal domain we term the SP-CTD. Structure-based m...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.07.013

    authors： Yunus AA,Lima CD

    更新日期：2009-09-11 00:00:00

  • A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends.

    abstract：:The mechanisms by which telomeres are distinguished from DNA double-strand breaks are poorly understood. Here we have defined the minimal requirements for the protection of telomeric DNA ends from nonhomologous end-joining (NHEJ). Neither long, single-stranded overhangs nor t loop formation is essential to prevent NHE...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.03.023

    authors： Bae NS,Baumann P

    更新日期：2007-05-11 00:00:00

  • Codon Usage and 3' UTR Length Determine Maternal mRNA Stability in Zebrafish.

    abstract：:The control of mRNA stability plays a central role in regulating gene expression. In metazoans, the earliest stages of development are driven by maternally supplied mRNAs. The degradation of these maternal mRNAs is critical for promoting the maternal-to-zygotic transition of developmental programs, although the underl...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.02.027

    authors： Mishima Y,Tomari Y

    更新日期：2016-03-17 00:00:00

  • A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.

    abstract：:CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.12.015

    authors： Wright AV,Wang JY,Burstein D,Harrington LB,Paez-Espino D,Kyrpides NC,Iavarone AT,Banfield JF,Doudna JA

    更新日期：2019-02-21 00:00:00

  • Epigenetic instability due to defective replication of structured DNA.

    abstract：:The accurate propagation of histone marks during chromosomal replication is proposed to rely on the tight coupling of replication with the recycling of parental histones to the daughter strands. Here, we show in the avian cell line DT40 that REV1, a key regulator of DNA translesion synthesis at the replication fork, i...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.11.009

    authors： Sarkies P,Reams C,Simpson LJ,Sale JE

    更新日期：2010-12-10 00:00:00

  • A BLAST from the past: ancient origin of human Sm proteins.

    abstract：:In this issue of Molecular Cell, Zhang et al. and Møller et al. independently report studies of the E. coli Hfq protein, revealing significant sequence similarities with human Sm proteins. Their findings suggest that Hfq, and the Sm and Sm-like (Lsm) proteins, may function in stabilizing interactions between RNA molec...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/s1097-2765(02)00429-x

    authors： Donahue WF,Jarrell KA

    更新日期：2002-01-01 00:00:00

  • Molecular Basis for Hormone Recognition and Activation of Corticotropin-Releasing Factor Receptors.

    abstract：:Corticotropin-releasing factor (CRF) and the three related peptides urocortins 1-3 (UCN1-UCN3) are endocrine hormones that control the stress responses by activating CRF1R and CRF2R, two members of class B G-protein-coupled receptors (GPCRs). Here, we present two cryoelectron microscopy (cryo-EM) structures of UCN1-bo...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2020.01.013

    authors： Ma S,Shen Q,Zhao LH,Mao C,Zhou XE,Shen DD,de Waal PW,Bi P,Li C,Jiang Y,Wang MW,Sexton PM,Wootten D,Melcher K,Zhang Y,Xu HE

    更新日期：2020-02-06 00:00:00

  • ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks.

    abstract：:Double-strand breaks (DSBs) of DNA in eukaryotic cells are predominantly repaired by non-homologous end joining (NHEJ). The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.08.021

    authors： Lee KY,Im JS,Shibata E,Dutta A

    更新日期：2017-10-05 00:00:00

  • MicroRNA pathways modulate polyglutamine-induced neurodegeneration.

    abstract：:Nine human neurodegenerative diseases are due to expansion of a CAG repeat- encoding glutamine within the open reading frame of the respective genes. Polyglutamine (polyQ) expansion confers dominant toxicity, resulting in neuronal degeneration. MicroRNAs (miRNAs) have been shown to modulate programmed cell death durin...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.07.030

    authors： Bilen J,Liu N,Burnett BG,Pittman RN,Bonini NM

    更新日期：2006-10-06 00:00:00

  • Transcriptional activation: mediator can act after preinitiation complex formation.

    abstract：:The Mediator complex connects transcriptional activators bound at enhancers with RNA polymerase (Pol) II. demonstrate that Mediator also has an important role in transcriptional activation after recruitment of the Pol II machinery to promoters. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章,评审

    doi：10.1016/j.molcel.2005.03.002

    authors： Struhl K

    更新日期：2005-03-18 00:00:00

  • Mitotic Transcription Installs Sgo1 at Centromeres to Coordinate Chromosome Segregation.

    abstract：:Human sister chromatids at metaphase are primarily linked by centromeric cohesion, forming the iconic X shape. Premature loss of centromeric cohesion disrupts orderly mitotic progression. Shugoshin (Sgo1) binds to and protects cohesin at inner centromeres. The kinetochore kinase Bub1 phosphorylates histone H2A at T120...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.06.018

    authors： Liu H,Qu Q,Warrington R,Rice A,Cheng N,Yu H

    更新日期：2015-08-06 00:00:00

  • PML, SUMO, and RNF4: guardians of nuclear protein quality.

    abstract：:In this issue of Molecular Cell, Guo et al. (2014) report that misfolded or aggregated nuclear proteins, such as pathogenic polyQ proteins, are cleared by a SUMO-dependent quality control pathway, which involves the E3 SUMO ligase PML and the SUMO-targeted ubiquitin ligase RNF4. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2014.06.022

    authors： Gärtner A,Muller S

    更新日期：2014-07-03 00:00:00

  • Stop codon recognition by release factors induces structural rearrangement of the ribosomal decoding center that is productive for peptide release.

    abstract：:Peptide release on the ribosome is catalyzed in the large subunit peptidyl transferase center by release factors on recognition of stop codons in the small subunit decoding center. Here we examine the role of the decoding center in this process. Mutation of decoding center nucleotides or removal of 2'OH groups from th...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.09.015

    authors： Youngman EM,He SL,Nikstad LJ,Green R

    更新日期：2007-11-30 00:00:00

  • TERRA -a calling card for telomerase.

    abstract：:Transcription of telomeric DNA has long been thought to inhibit telomerase. Using live cell imaging, in this issue, Cusanelli et al. (2013) now reveal a different scenario: telomeric RNA is preferentially generated at short telomeres and delivers telomerase to the chromosome end from which the transcript originated. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2013.09.011

    authors： Smekalova E,Baumann P

    更新日期：2013-09-26 00:00:00

  • Acetylation regulates transcription factor activity at multiple levels.

    abstract：:CREB-binding protein (CBP) possesses an intrinsic acetyltransferase activity capable of acetylating nucleosomal histones as well as several nonhistone proteins. Here, it is shown that CBP can acetylate hepatocyte nuclear factor-4 (HNF-4), a member of the nuclear hormone receptor family, at lysine residues within the n...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80253-1

    authors： Soutoglou E,Katrakili N,Talianidis I

    更新日期：2000-04-01 00:00:00

  • Insights into FlaI functions in archaeal motor assembly and motility from structures, conformations, and genetics.

    abstract：:Superfamily ATPases in type IV pili, type 2 secretion, and archaella (formerly archaeal flagella) employ similar sequences for distinct biological processes. Here, we structurally and functionally characterize prototypical superfamily ATPase FlaI in Sulfolobus acidocaldarius, showing FlaI activities in archaeal swimmi...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.01.014

    authors： Reindl S,Ghosh A,Williams GJ,Lassak K,Neiner T,Henche AL,Albers SV,Tainer JA

    更新日期：2013-03-28 00:00:00

  • miR-200c regulates induction of apoptosis through CD95 by targeting FAP-1.

    abstract：:Tumor progression shares many characteristics with the process of epithelial-to-mesenchymal transition (EMT). Cells that have undergone an EMT are known to have an increased resistance to apoptosis. CD95/Fas is an apoptosis-inducing receptor expressed on many tissues and tumor cells. During tumor progression CD95 is f...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.05.018

    authors： Schickel R,Park SM,Murmann AE,Peter ME

    更新日期：2010-06-25 00:00:00

  • Polycomb-Dependent Chromatin Looping Contributes to Gene Silencing during Drosophila Development.

    abstract：:Interphase chromatin is organized into topologically associating domains (TADs). Within TADs, chromatin looping interactions are formed between DNA regulatory elements, but their functional importance for the establishment of the 3D genome organization and gene regulation during development is unclear. Using high-reso...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.05.032

    authors： Ogiyama Y,Schuettengruber B,Papadopoulos GL,Chang JM,Cavalli G

    更新日期：2018-07-05 00:00:00

  • Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes.

    abstract：:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA pr...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.11.006

    authors： Egloff S,Zaborowska J,Laitem C,Kiss T,Murphy S

    更新日期：2012-01-13 00:00:00

  • An Arginine Nexus in the RNA Polymerase II CTD.

    abstract：:In this issue of Molecular Cell,Sharma et al. (2019) show that normal cell growth requires conversion of an arginine residue in the RNA polymerase II C-terminal domain (CTD) to citrulline, uncovering a potential regulatory pathway involving opposing arginine modifications. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2018.12.013

    authors： Corden JL

    更新日期：2019-01-03 00:00:00

  • AUF1/HnRNP D RNA binding protein functions in telomere maintenance.

    abstract：:In this issue of Molecular Cell, Pont et al. (2012) show that AUF1/hnRNP D promotes TERT transcription, which is required for telomere maintenance in mice. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2012.06.031

    authors： Chen LY,Lingner J

    更新日期：2012-07-13 00:00:00

  • Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1.

    abstract：:Microtubule plus end binding proteins (+TIPs) localize to the dynamic plus ends of microtubules, where they stimulate microtubule growth and recruit signaling molecules. Three main +TIP classes have been identified (XMAP215, EB1, and CLIP-170), but whether they act upon microtubule plus ends through a similar mechanis...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.07.023

    authors： Slep KC,Vale RD

    更新日期：2007-09-21 00:00:00

  • Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.

    abstract：:In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this t...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.01.001

    authors： Madabhushi R,Marians KJ

    更新日期：2009-01-30 00:00:00

  • The special delivery of a tail-anchored protein: why it pays to use a dedicated courier.

    abstract：:The membrane-spanning C-terminal regions in tail-anchored proteins must be recognized and delivered posttranslationally to the endoplasmic reticulum or mitochondrial membrane. A paper in this issue of Molecular Cell (Wang et al., 2010) and another recent report (Mariappan et al., 2010) delineate early steps in this pa...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.09.016

    authors： Brodsky JL

    更新日期：2010-10-08 00:00:00

  • SMADs and YAP compete to control elongation of β-catenin:LEF-1-recruited RNAPII during hESC differentiation.

    abstract：:The Wnt3a/β-catenin and Activin/SMAD2,3 signaling pathways synergize to induce endodermal differentiation of human embryonic stem cells; however, the underlying mechanism is not well understood. Using ChIP-seq and GRO-seq analyses, we show here that Wnt3a-induced β-catenin:LEF-1 enhancers recruit cohesin to direct enh...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.04.001

    authors： Estarás C,Benner C,Jones KA

    更新日期：2015-06-04 00:00:00

  • Eukaryotic stress granules: the ins and outs of translation.

    abstract：:The stress response in eukaryotic cells often inhibits translation initiation and leads to the formation of cytoplasmic RNA-protein complexes referred to as stress granules. Stress granules contain nontranslating mRNAs, translation initiation components, and many additional proteins affecting mRNA function. Stress gra...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2009.11.020

    authors： Buchan JR,Parker R

    更新日期：2009-12-25 00:00:00

  • RhoA GTPase regulates B cell receptor signaling.

    abstract：:The RhoA GTPase controls many cellular functions, including gene transcription and actin polymerization. Several lines of evidence suggest that Rho GTPases are required for B cell receptor (BCR) signaling, but whether RhoA is necessary has not been investigated. Here, we show that RhoA is activated, downstream of PI3K...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.12.012

    authors： Saci A,Carpenter CL

    更新日期：2005-01-21 00:00:00

  • A major conformational change in p97 AAA ATPase upon ATP binding.

    abstract：:AAA ATPases play central roles in cellular activities. The ATPase p97, a prototype of this superfamily, participates in organelle membrane fusion. Cryoelectron microscopy and single-particle analysis revealed that a major conformational change of p97 during the ATPase cycle occurred upon nucleotide binding and not dur...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)00144-1

    authors： Rouiller I,Butel VM,Latterich M,Milligan RA,Wilson-Kubalek EM

    更新日期：2000-12-01 00:00:00