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A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.

Abstract：

:CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. Most type V-C and V-D systems lack the cas2 gene and have unusually short CRISPR repeats and spacers. Here, we show that a mini-integrase comprising the type V-C Cas1 protein alone catalyzes DNA integration with a preference for short (17- to 19-base-pair) DNA fragments. The mini-integrase has weak specificity for the CRISPR array. We present evidence that the Cas1 proteins form a tetramer for integration. Our findings support a model of a minimal integrase with an internal ruler mechanism that favors shorter repeats and spacers. This minimal integrase may represent the function of the ancestral Cas1 prior to Cas2 adoption.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Wright AV,Wang JY,Burstein D,Harrington LB,Paez-Espino D,Kyrpides NC,Iavarone AT,Banfield JF,Doudna JA

doi

10.1016/j.molcel.2018.12.015

subject

Has Abstract

pub_date

2019-02-21 00:00:00

pages

727-737.e3

issue

4

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(18)31069-4

journal_volume

73

pub_type

杂志文章

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