Abstract：
:The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Here, we use DNA microarrays to explore the genome-wide interplay of several TBP-interacting inhibitors in the yeast Saccharomyces cerevisiae. Our findings suggest the following: The NC2 inhibitor turns down, but not off, highly active genes. Autoinhibition of TBP through dimerization contributes to transcriptional repression, even at repressive subtelomeric regions. The TAND domain of TAF1 plays a primary inhibitory role at very few genes, but its function becomes widespread when other TBP interactions are compromised. These findings reveal that transcriptional output is limited in part by a collaboration of different combinations of TBP inhibitory mechanisms.
journal_name
Mol Celljournal_title
Molecular cellauthors
Chitikila C,Huisinga KL,Irvin JD,Basehoar AD,Pugh BFdoi
10.1016/s1097-2765(02)00683-4subject
Has Abstractpub_date
2002-10-01 00:00:00pages
871-82issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(02)00683-4journal_volume
10pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:Corticotropin-releasing factor (CRF) and the three related peptides urocortins 1-3 (UCN1-UCN3) are endocrine hormones that control the stress responses by activating CRF1R and CRF2R, two members of class B G-protein-coupled receptors (GPCRs). Here, we present two cryoelectron microscopy (cryo-EM) structures of UCN1-bo...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.01.013
更新日期：2020-02-06 00:00:00
abstract：:Centromeres are specialized chromatin domains specified by the centromere-specific CENP-A nucleosome. The stable inheritance of vertebrate centromeres is an epigenetic process requiring deposition of new CENP-A nucleosomes by HJURP. We show HJURP is recruited to centromeres through a direct interaction between the HJU...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.02.014
更新日期：2016-03-03 00:00:00
abstract：:The HECT E3 ligases ubiquitinate numerous transcription factors and signaling molecules, and their activity must be tightly controlled to prevent cancer, immune disorders, and other diseases. In this study, we have found unexpectedly that peptide linkers tethering WW domains in several HECT family members are key regu...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.03.020
更新日期：2017-05-04 00:00:00
abstract：:The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas nuclease system is a powerful tool for genome editing, and its simple programmability has enabled high-throughput genetic and epigenetic studies. These high-throughput approaches offer investigators a toolkit for functional interrogation of not...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2017.09.017
更新日期：2017-10-05 00:00:00
abstract：:Rad51 is a DNA recombinase functioning in the repair of DNA double-strand breaks and the generation of genetic diversity by homologous recombination (HR). In the presence of ATP, Rad51 self-assembles into an extended polymer on single-stranded DNA to catalyze strand exchange. Inappropriate HR causes genomic instabilit...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.05.026
更新日期：2009-07-10 00:00:00
abstract：:Nuclear export of mRNA in eukaryotic cells is mediated by soluble transport factors and components of the nuclear pore complex (NPC). The cytoplasmically oriented nuclear pore protein Nup159 plays a critical role in mRNA export through its conserved N-terminal domain (NTD). Here, we report the crystal structure of the...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2004.10.032
更新日期：2004-12-03 00:00:00
abstract：:Heterochromatin formation is generally thought to result in transcriptional repression of target loci. However, RNAi-mediated heterochromatin assembly requires RNA polymerase II (Pol II) transcription. The mechanism facilitating Pol II accessibility to heterochromatin is unknown. We show that the fission yeast Epe1, a...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.05.010
更新日期：2006-06-09 00:00:00
abstract：:While gapmers efficiently knock down as well as terminate transcription of nascent lncRNAs and mRNAs, Lee and Mendell (2020) and Lai et al. (2020) also demonstrate that Pol II termination is not observed with gapmers targeting the 3' terminal portions of the transcript. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2020.02.010
更新日期：2020-03-05 00:00:00
abstract：:The anaphase-promoting complex or cyclosome (APC/C) is a ubiquitin ligase that polyubiquitinates specific substrates at precise times in the cell cycle, thereby triggering the events of late mitosis in a strict order. The robust substrate specificity of the APC/C prevents the potentially deleterious degradation of non...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2016.09.006
更新日期：2016-10-06 00:00:00
abstract：:Group II intron RNPs are mobile genetic elements that attack and invade duplex DNA. In this work, we monitor the invasion reaction in vitro and establish a quantitative kinetic framework for the steps of this complex cascade. We find that target site specificity is achieved after DNA binding, which occurs nonspecifica...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00069-8
更新日期：2003-03-01 00:00:00
abstract：:Anti-sense transcription originating upstream of mammalian protein-coding genes is a well-documented phenomenon, but remarkably little is known about the regulation or function of anti-sense promoters and the non-coding RNAs they generate. Here we define at nucleotide resolution the divergent transcription start sites...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2015.04.006
更新日期：2015-06-18 00:00:00
abstract：:Covalently closed circular RNAs (circRNAs) are produced by precursor mRNA back-splicing of exons of thousands of genes in eukaryotes. circRNAs are generally expressed at low levels and often exhibit cell-type-specific and tissue-specific patterns. Recent studies have shown that their biogenesis requires spliceosomal m...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2018.06.034
更新日期：2018-08-02 00:00:00
abstract：:Mutations in Hnf-1alpha are the most common Mendelian cause of diabetes mellitus. To elucidate the molecular function of a mutational hotspot, we cocrystallized human HNF-1alpha 83-279 with a high-affinity promoter and solved the structure of the complex. Two identical protein molecules are bound to the promoter. Each...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00704-9
更新日期：2002-11-01 00:00:00
abstract：:In mammals, inactivation of one X chromosome in the female equalizes gene dosages between XX females and XY males. Two noncoding loci, Tsix and Xite, together regulate X chromosome fate by controlling homologous chromosome pairing, counting, and mutually exclusive choice. Following choice, the asymmetry of Xite and Ts...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.11.017
更新日期：2007-01-12 00:00:00
abstract：:An exciting study in this issue reveals how binding of the microtubule associated protein TPX2 to the mitotic kinase Aurora A induces a conformational change. This moves the phosphorylated activation domain into a more compact position within the kinase core, providing a better substrate binding platform and hiding th...
journal_title：Molecular cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s1097-2765(03)00405-2
更新日期：2003-10-01 00:00:00
abstract：:The proper processing, export, localization, translation, and degradation of mRNAs are necessary for regulation of gene expression. These processes are controlled by mRNA-specific regulatory proteins, noncoding RNAs, and core machineries common to most mRNAs. These factors bind the mRNA in large complexes known as mes...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2014.04.033
更新日期：2014-05-22 00:00:00
abstract：:Atg7 is a noncanonical, homodimeric E1 enzyme that interacts with the noncanonical E2 enzyme, Atg3, to mediate conjugation of the ubiquitin-like protein (UBL) Atg8 during autophagy. Here we report that the unique N-terminal domain of Atg7 (Atg7(NTD)) recruits a unique "flexible region" from Atg3 (Atg3(FR)). The struct...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.08.034
更新日期：2011-11-04 00:00:00
abstract：:Staphylococcus aureus virulence is regulated when secreted autoinducing peptides (AIPs) are recognized by a membrane-bound receptor histidine kinase (RHK), AgrC. Some AIPs are agonists of virulence gene expression, while others are antagonists. It is unclear how AIP binding regulates AgrC activity. Here, we reconstitu...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2014.02.029
更新日期：2014-03-20 00:00:00
abstract：:Solving the biological roles of covalent histone modifications, including monoubiquitination of histone H2A, and the molecular mechanisms by which these modifications regulate specific transcriptional programs remains a central question for all eukaryotes. Here we report that the N-CoR/HDAC1/3 complex specifically rec...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.11.002
更新日期：2008-01-18 00:00:00
abstract：:A truly groundbreaking paper by Pisarev et al. (2007), recently published in Cell, has solved a wide gap in our knowledge of eukaryotic mRNA translation, by elucidating how ribosomes are released from mRNA after the termination step. ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2007.10.014
更新日期：2007-11-09 00:00:00
abstract：:In a recent publication in Science, Kujirai et al. (2018) use single-particle cryo-EM to resolve several Pol II-nucleosome interactions, shedding new light on transcription elongation in a native chromatin environment. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2018.11.027
更新日期：2018-12-06 00:00:00
abstract：:During transcription initiation by bacterial RNA polymerase, the sigma subunit recognizes the -35 and -10 promoter elements; free sigma, however, does not bind DNA. We selected ssDNA aptamers that strongly and specifically bound free sigma(A) from Thermus aquaticus. A consensus sequence, GTA(C/T)AATGGGA, was required ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.06.010
更新日期：2006-07-07 00:00:00
abstract：:Type II topoisomerases are essential for resolving topologically entwined double-stranded DNA. Although anti-topoisomerase 2 (Top2) drugs are clinically important antibiotics and chemotherapies, to our knowledge, the mechanisms of cell killing by Top2 depletion and inactivation have never been directly compared. We sh...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2008.04.019
更新日期：2008-06-20 00:00:00
abstract：:Although DNA looping between the initiator binding sites (iterons) of the replication origin (ori) of a plasmid and the iterons located in a cis-acting control sequence called inc has been postulated to promote negative control of plasmid DNA replication, not only was definitive evidence for such looping lacking, but ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.10.037
更新日期：2005-12-22 00:00:00
abstract：:Steroid hormones have rapid nongenomic effects on cell-signaling pathways, but the receptor mechanisms responsible for this are not understood. We have identified a specific polyproline motif in the amino-terminal domain of conventional progesterone receptor (PR) that mediates direct progestin-dependent interaction of...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(01)00304-5
更新日期：2001-08-01 00:00:00
abstract：:The Mediator complex connects transcriptional activators bound at enhancers with RNA polymerase (Pol) II. demonstrate that Mediator also has an important role in transcriptional activation after recruitment of the Pol II machinery to promoters. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.molcel.2005.03.002
更新日期：2005-03-18 00:00:00
abstract：:Progressive phosphorylation of circadian clock proteins is a hallmark of time-keeping. In this issue of Molecular Cell, Querfurth et al. (2011) demonstrate that phosphorylation of Neurospora FRQ induces a conformational change, which can account for its temporally gated degradation. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2011.08.015
更新日期：2011-09-02 00:00:00
abstract：:The autophagy cargo receptor p62 facilitates the condensation of misfolded, ubiquitin-positive proteins and their degradation by autophagy, but the molecular mechanism of p62 signaling to the core autophagy machinery is unclear. Here, we show that disordered residues 326-380 of p62 directly interact with the C-termina...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.01.035
更新日期：2019-04-18 00:00:00
abstract：:Sphingosine 1-phosphate (S1P) functions not only as a bioactive lipid molecule, but also as an important intermediate of the sole sphingolipid-to-glycerolipid metabolic pathway. However, the precise reactions and the enzymes involved in this pathway remain unresolved. We report here that yeast HFD1 and the Sjögren-Lar...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.04.033
更新日期：2012-05-25 00:00:00
abstract：:During X-inactivation, Xist RNA spreads along an entire chromosome to establish silencing. However, the mechanism and functional RNA elements involved in spreading remain undefined. By performing a comprehensive endogenous Xist deletion screen, we identify Repeat B as crucial for spreading Xist and maintaining Polycom...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.01.015
更新日期：2019-04-04 00:00:00
