当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > FIP200 Claw Domain Binding to p62 Promotes Autophagosome Formation at Ubiquitin Condensates.

FIP200 Claw Domain Binding to p62 Promotes Autophagosome Formation at Ubiquitin Condensates.

Abstract：

:The autophagy cargo receptor p62 facilitates the condensation of misfolded, ubiquitin-positive proteins and their degradation by autophagy, but the molecular mechanism of p62 signaling to the core autophagy machinery is unclear. Here, we show that disordered residues 326-380 of p62 directly interact with the C-terminal region (CTR) of FIP200. Crystal structure determination shows that the FIP200 CTR contains a dimeric globular domain that we designated the "Claw" for its shape. The interaction of p62 with FIP200 is mediated by a positively charged pocket in the Claw, enhanced by p62 phosphorylation, mutually exclusive with the binding of p62 to LC3B, and it promotes degradation of ubiquitinated cargo by autophagy. Furthermore, the recruitment of the FIP200 CTR slows the phase separation of ubiquitinated proteins by p62 in a reconstituted system. Our data provide the molecular basis for a crosstalk between cargo condensation and autophagosome formation.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Turco E,Witt M,Abert C,Bock-Bierbaum T,Su MY,Trapannone R,Sztacho M,Danieli A,Shi X,Zaffagnini G,Gamper A,Schuschnig M,Fracchiolla D,Bernklau D,Romanov J,Hartl M,Hurley JH,Daumke O,Martens S

doi

10.1016/j.molcel.2019.01.035

subject

Has Abstract

pub_date

2019-04-18 00:00:00

pages

330-346.e11

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(19)30055-3

journal_volume

74

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs.

    abstract：:Upon glucose restriction, eukaryotic cells upregulate oxidative metabolism to maintain homeostasis. Using genetic screens, we find that the mitochondrial serine hydroxymethyltransferase (SHMT2) is required for robust mitochondrial oxygen consumption and low glucose proliferation. SHMT2 catalyzes the first step in mito...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.01.024

    authors： Minton DR,Nam M,McLaughlin DJ,Shin J,Bayraktar EC,Alvarez SW,Sviderskiy VO,Papagiannakopoulos T,Sabatini DM,Birsoy K,Possemato R

    更新日期：2018-02-15 00:00:00

  • Robust Ordering of Anaphase Events by Adaptive Thresholds and Competing Degradation Pathways.

    abstract：:The splitting of chromosomes in anaphase and their delivery into the daughter cells needs to be accurately executed to maintain genome stability. Chromosome splitting requires the degradation of securin, whereas the distribution of the chromosomes into the daughter cells requires the degradation of cyclin B. We show t...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.09.022

    authors： Kamenz J,Mihaljev T,Kubis A,Legewie S,Hauf S

    更新日期：2015-11-05 00:00:00

  • Histone acetylation by HBO1 tightens replication licensing.

    abstract：:In this issue of Molecular Cell, Miotto and Struhl (2010) suggest that replication licensing, the loading of Mcm2-7 onto DNA, is promoted by HBO1 acetylating histone H4 at replication origins, providing a molecular view of how chromatin status influences origin usage. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2009.12.028

    authors： Chadha GS,Blow JJ

    更新日期：2010-01-15 00:00:00

  • Stop codon recognition by release factors induces structural rearrangement of the ribosomal decoding center that is productive for peptide release.

    abstract：:Peptide release on the ribosome is catalyzed in the large subunit peptidyl transferase center by release factors on recognition of stop codons in the small subunit decoding center. Here we examine the role of the decoding center in this process. Mutation of decoding center nucleotides or removal of 2'OH groups from th...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.09.015

    authors： Youngman EM,He SL,Nikstad LJ,Green R

    更新日期：2007-11-30 00:00:00

  • A role for the deep orange and carnation eye color genes in lysosomal delivery in Drosophila.

    abstract：:Deep orange and carnation are two of the classic eye color genes in Drosophila. Here, we demonstrate that Deep orange is part of a protein complex that localizes to endosomal compartments. A second component of this complex is Carnation, a homolog of Sec1p-like regulators of membrane fusion. Because complete loss of d...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80199-9

    authors： Sevrioukov EA,He JP,Moghrabi N,Sunio A,Krämer H

    更新日期：1999-10-01 00:00:00

  • Ribonucleotide Reductase Requires Subunit Switching in Hypoxia to Maintain DNA Replication.

    abstract：:Cells exposed to hypoxia experience replication stress but do not accumulate DNA damage, suggesting sustained DNA replication. Ribonucleotide reductase (RNR) is the only enzyme capable of de novo synthesis of deoxyribonucleotide triphosphates (dNTPs). However, oxygen is an essential cofactor for mammalian RNR (RRM1/RR...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.03.005

    authors： Foskolou IP,Jorgensen C,Leszczynska KB,Olcina MM,Tarhonskaya H,Haisma B,D'Angiolella V,Myers WK,Domene C,Flashman E,Hammond EM

    更新日期：2017-04-20 00:00:00

  • Fluorescence Amplification Method for Forward Genetic Discovery of Factors in Human mRNA Degradation.

    abstract：:Nonsense-mediated decay (NMD) degrades mRNAs containing a premature termination codon (PTC). PTCs are a frequent cause of human genetic diseases, and the NMD pathway is known to modulate disease severity. Since partial NMD attenuation can potentially enhance nonsense suppression therapies, better definition of human-s...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.11.032

    authors： Alexandrov A,Shu MD,Steitz JA

    更新日期：2017-01-05 00:00:00

  • Meiotic recombination remote from prominent DNA break sites in S. pombe.

    abstract：:DNA breakage is intimately associated with meiotic recombination in the fission yeast Schizosaccharomyces pombe. Sites of prominent DNA breakage were found approximately 25 to approximately 200 kb apart in the genomic regions surveyed. We examined in detail a 501 kb region of chromosome I and found six sites, or tight...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(02)00452-5

    authors： Young JA,Schreckhise RW,Steiner WW,Smith GR

    更新日期：2002-02-01 00:00:00

  • Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes.

    abstract：:The Mre11/Rad50/Nbs1 complex initiates double-strand break repair by homologous recombination (HR). Loss of Mre11 or its nuclease activity in mouse cells is known to cause genome aberrations and cellular senescence, although the molecular basis for this phenotype is not clear. To identify the origin of these defects, ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.10.011

    authors： Hoa NN,Shimizu T,Zhou ZW,Wang ZQ,Deshpande RA,Paull TT,Akter S,Tsuda M,Furuta R,Tsutsui K,Takeda S,Sasanuma H

    更新日期：2016-11-03 00:00:00

  • Non-catalytic Roles for XPG with BRCA1 and BRCA2 in Homologous Recombination and Genome Stability.

    abstract：:XPG is a structure-specific endonuclease required for nucleotide excision repair, and incision-defective XPG mutations cause the skin cancer-prone syndrome xeroderma pigmentosum. Truncating mutations instead cause the neurodevelopmental progeroid disorder Cockayne syndrome, but little is known about how XPG loss resul...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.12.026

    authors： Trego KS,Groesser T,Davalos AR,Parplys AC,Zhao W,Nelson MR,Hlaing A,Shih B,Rydberg B,Pluth JM,Tsai MS,Hoeijmakers JHJ,Sung P,Wiese C,Campisi J,Cooper PK

    更新日期：2016-02-18 00:00:00

  • Coupling of ribosomal L1 stalk and tRNA dynamics during translation elongation.

    abstract：:By using single-molecule fluorescence resonance energy transfer (smFRET), we observe the real-time dynamic coupling between the ribosome, labeled at the L1 stalk, and transfer RNA (tRNA). We find that an interaction between the ribosomal L1 stalk and the newly deacylated tRNA is established spontaneously upon peptide ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.03.012

    authors： Fei J,Kosuri P,MacDougall DD,Gonzalez RL Jr

    更新日期：2008-05-09 00:00:00

  • SR proteins function in coupling RNAP II transcription to pre-mRNA splicing.

    abstract：:Transcription and splicing are functionally coupled, resulting in highly efficient splicing of RNA polymerase II (RNAP II) transcripts. The mechanism involved in this coupling is not known. To identify potential coupling factors, we carried out a comprehensive proteomic analysis of immunopurified human RNAP II, identi...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.05.036

    authors： Das R,Yu J,Zhang Z,Gygi MP,Krainer AR,Gygi SP,Reed R

    更新日期：2007-06-22 00:00:00

  • Oncogenic Ras and Akt signaling contribute to glioblastoma formation by differential recruitment of existing mRNAs to polysomes.

    abstract：:In order to determine the global effects of oncogenic Ras and Akt signaling pathways on translational efficiencies, we compared the gene expression profiles of total cellular mRNA and mRNA associated with polysomes. We found that the immediate effect of Ras and Akt signaling blockade on transcription was relatively mo...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00395-2

    authors： Rajasekhar VK,Viale A,Socci ND,Wiedmann M,Hu X,Holland EC

    更新日期：2003-10-01 00:00:00

  • A critical role for noncoding 5S rRNA in regulating Mdmx stability.

    abstract：:Both p53 and Mdmx are ubiquitinated and degraded by the same E3 ligase Mdm2; interestingly, however, while p53 is rapidly degraded by Mdm2, Mdmx is a stable protein in most cancer cells. Thus, the mechanism by which Mdmx is degraded by Mdm2 needs further elucidation. Here, we identified the noncoding 5S rRNA as a majo...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.08.020

    authors： Li M,Gu W

    更新日期：2011-09-16 00:00:00

  • The response regulator BvgA and RNA polymerase alpha subunit C-terminal domain bind simultaneously to different faces of the same segment of promoter DNA.

    abstract：:Examination of the binding of FeBABE-conjugated BvgA to the fha promoter of Bordetella pertussis has revealed that three dimers, formed by head-to-head association of monomers, bind one face of the DNA helix from the inverted-heptad primary binding site to the -35 region. The orientation of BvgA monomers within the di...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00007-8

    authors： Boucher PE,Maris AE,Yang MS,Stibitz S

    更新日期：2003-01-01 00:00:00

  • Transcription Factor-Directed Re-wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming toward trans-Differentiation.

    abstract：:MYOD-directed fibroblast trans-differentiation into skeletal muscle provides a unique model to investigate how one transcription factor (TF) reconfigures the three-dimensional chromatin architecture to control gene expression, which is otherwise achieved by the combinatorial activities of multiple TFs. Integrative ana...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2019.07.036

    authors： Dall'Agnese A,Caputo L,Nicoletti C,di Iulio J,Schmitt A,Gatto S,Diao Y,Ye Z,Forcato M,Perera R,Bicciato S,Telenti A,Ren B,Puri PL

    更新日期：2019-11-07 00:00:00

  • Spatial and temporal recruitment of androgen receptor and its coactivators involves chromosomal looping and polymerase tracking.

    abstract：:Androgen receptor (AR) plays a critical role in the development and progression of prostate cancer, where it is a key therapeutic target. Here we report that, in contrast to estrogen receptor transcription complexes which form within minutes and recycle hourly, the levels of regulatory regions bound by AR complexes ri...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.07.018

    authors： Wang Q,Carroll JS,Brown M

    更新日期：2005-09-02 00:00:00

  • Retrotransposon Tf1 is targeted to Pol II promoters by transcription activators.

    abstract：:The LTR-retrotransposon Tf1 preserves the coding capacity of its host Schizosaccharomyces pombe by integrating upstream of open reading frames (ORFs). To determine which features of the target sites were recognized by the transposon, we introduced plasmids containing candidate insertion sites into S. pombe and mapped ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2008.02.016

    authors： Leem YE,Ripmaster TL,Kelly FD,Ebina H,Heincelman ME,Zhang K,Grewal SI,Hoffman CS,Levin HL

    更新日期：2008-04-11 00:00:00

  • PRDM16 Suppresses MLL1r Leukemia via Intrinsic Histone Methyltransferase Activity.

    abstract：:PRDM16 is a transcription co-factor that plays critical roles in development of brown adipose tissue, as well as maintenance of adult hematopoietic and neural stem cells. Here we report that PRDM16 is a histone H3K4 methyltransferase on chromatin. Mutation in the N-terminal PR domain of PRDM16 abolishes the intrinsic ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2016.03.010

    authors： Zhou B,Wang J,Lee SY,Xiong J,Bhanu N,Guo Q,Ma P,Sun Y,Rao RC,Garcia BA,Hess JL,Dou Y

    更新日期：2016-04-21 00:00:00

  • Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication.

    abstract：:Gene-targeted knockout mice have been generated lacking the major uracil-DNA glycosylase, UNG. In contrast to ung- mutants of bacteria and yeast, such mice do not exhibit a greatly increased spontaneous mutation frequency. However, there is only slow removal of uracil from misincorporated dUMP in isolated ung-/- nucle...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80271-3

    authors： Nilsen H,Rosewell I,Robins P,Skjelbred CF,Andersen S,Slupphaug G,Daly G,Krokan HE,Lindahl T,Barnes DE

    更新日期：2000-06-01 00:00:00

  • Insights into strand displacement and processivity from the crystal structure of the protein-primed DNA polymerase of bacteriophage phi29.

    abstract：:The DNA polymerase from phage phi29 is a B family polymerase that initiates replication using a protein as a primer, attaching the first nucleotide of the phage genome to the hydroxyl of a specific serine of the priming protein. The crystal structure of phi29 DNA polymerase determined at 2.2 A resolution provides expl...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.10.019

    authors： Kamtekar S,Berman AJ,Wang J,Lázaro JM,de Vega M,Blanco L,Salas M,Steitz TA

    更新日期：2004-11-19 00:00:00

  • Viral-mediated noisy gene expression reveals biphasic E2f1 response to MYC.

    abstract：:Gene expression mediated by viral vectors is subject to cell-to-cell variability, which limits the accuracy of gene delivery. When coupled with single-cell measurements, however, such variability provides an efficient means to quantify signaling dynamics in mammalian cells. Here, we illustrate the utility of this appr...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.01.014

    authors： Wong JV,Yao G,Nevins JR,You L

    更新日期：2011-02-04 00:00:00

  • Dynamics of substrate denaturation and translocation by the ClpXP degradation machine.

    abstract：:ClpXP is a protein machine composed of the ClpX ATPase, a member of the Clp/Hsp100 family of remodeling enzymes, and the ClpP peptidase. Here, ClpX and ClpXP are shown to catalyze denaturation of GFP modified with an ssrA degradation tag. ClpX translocates this denatured protein into the proteolytic chamber of ClpP an...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80243-9

    authors： Kim YI,Burton RE,Burton BM,Sauer RT,Baker TA

    更新日期：2000-04-01 00:00:00

  • A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits.

    abstract：:Analyses of isolated pre-ribosomes yielded biochemical "snapshots" of the dynamic, nascent 60S and 40S subunits during their path from the nucleolus to the cytoplasm. Here, we present the structure of a pre-60S ribosomal intermediate located in the nucleoplasm. A huge dynein-related AAA-type ATPase (Rea1) and the Rix1...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.06.033

    authors： Nissan TA,Galani K,Maco B,Tollervey D,Aebi U,Hurt E

    更新日期：2004-07-23 00:00:00

  • UneCLIPsing HuR nuclear function.

    abstract：:The RNA-binding protein HuR, while known to stabilize cytoplasmic mRNAs, is largely nuclear. In this issue of Molecular Cell, Mukherjee et al. (2011) and Lebedeva et al. (2011) identify transcriptome-wide HuR-RNA interactions using PAR-CLIP, unveiling HuR's nuclear role in pre-mRNA processing. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2011.07.016

    authors： Srikantan S,Gorospe M

    更新日期：2011-08-05 00:00:00

  • Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300.

    abstract：:The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, an...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80066-0

    authors： Zhong H,Voll RE,Ghosh S

    更新日期：1998-04-01 00:00:00

  • Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O.

    abstract：:The tumor suppressor BAP1 interacts with chromatin-associated proteins and regulates cell proliferation, but its mechanism of action and regulation remain poorly defined. We show that the ubiquitin-conjugating enzyme UBE2O multi-monoubiquitinates the nuclear localization signal of BAP1, thereby inducing its cytoplasmi...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.03.002

    authors： Mashtalir N,Daou S,Barbour H,Sen NN,Gagnon J,Hammond-Martel I,Dar HH,Therrien M,Affar el B

    更新日期：2014-05-08 00:00:00

  • Epigenetic instability due to defective replication of structured DNA.

    abstract：:The accurate propagation of histone marks during chromosomal replication is proposed to rely on the tight coupling of replication with the recycling of parental histones to the daughter strands. Here, we show in the avian cell line DT40 that REV1, a key regulator of DNA translesion synthesis at the replication fork, i...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.11.009

    authors： Sarkies P,Reams C,Simpson LJ,Sale JE

    更新日期：2010-12-10 00:00:00

  • Srs2 disassembles Rad51 filaments by a protein-protein interaction triggering ATP turnover and dissociation of Rad51 from DNA.

    abstract：:Rad51 is a DNA recombinase functioning in the repair of DNA double-strand breaks and the generation of genetic diversity by homologous recombination (HR). In the presence of ATP, Rad51 self-assembles into an extended polymer on single-stranded DNA to catalyze strand exchange. Inappropriate HR causes genomic instabilit...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.05.026

    authors： Antony E,Tomko EJ,Xiao Q,Krejci L,Lohman TM,Ellenberger T

    更新日期：2009-07-10 00:00:00

  • Fluoxetine-resistant mutants in C. elegans define a novel family of transmembrane proteins.

    abstract：:Fluoxetine (Prozac) is an antidepressant that is thought to act by blocking presynaptic reuptake of the neurotransmitter serotonin. Despite widespread clinical use of fluoxetine, direct evidence for this mechanism has been difficult to obtain in vivo. We have determined that fluoxetine has an additional neuromuscular ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)80362-7

    authors： Choy RK,Thomas JH

    更新日期：1999-08-01 00:00:00